A5053339236- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- HIV Research and Treatment
- HIV-related health complications and treatments
- Blood groups and transfusion
- Cytomegalovirus and herpesvirus research
- HIV, Drug Use, Sexual Risk
- Sirtuins and Resveratrol in Medicine
- Autophagy in Disease and Therapy
- Biosimilars and Bioanalytical Methods
- Ovarian function and disorders
- Reproductive Biology and Fertility
- Cellular transport and secretion
- Virus-based gene therapy research
- Heavy Metal Exposure and Toxicity
- Peripheral Neuropathies and Disorders
- Ginseng Biological Effects and Applications
- Flavonoids in Medical Research
- RNA Interference and Gene Delivery
- Autoimmune Neurological Disorders and Treatments
- Calcium signaling and nucleotide metabolism
- Surgical Sutures and Adhesives
- Radiation Shielding Materials Analysis
- Nuclear materials and radiation effects
- Neurological Disease Mechanisms and Treatments
University of Montana
2013-2025
University of Colorado Anschutz Medical Campus
2012-2025
Zhejiang University
2023
Yiwu Science and Technology Research Institute
2023
Shantou University
2021
Shantou University Medical College
2021
Second Hospital of Hebei Medical University
2017-2019
Hebei Medical University
2017-2019
Nanning Normal University
2015-2018
University of Colorado Denver
2010-2016
Antiretroviral chemoprophylaxis before exposure is a promising approach for the prevention of human immunodeficiency virus (HIV) acquisition.
Tenofovir (TFV) disoproxil fumarate (TDF)±emtricitabine (FTC) are widely used for HIV treatment and chemoprophylaxis, but variable adherence may lead to suboptimal responses. Methods that quantify would allow interventions improve prevention outcomes. Our objective was characterize the pharmacokinetics of TFV-diphosphate (TFV-DP) FTC-triphosphate (FTC-TP) in red blood cells (RBCs) peripheral mononuclear (PBMCs); extend RBC analysis dried spots (DBSs); model how RBC/DBS monitoring could...
The pharmacokinetics (PK) of tenofovir-diphosphate (TFV-DP) and emtricitabine-triphosphate (FTC-TP), the active anabolites tenofovir disoproxil fumarate (TDF), emtricitabine (FTC) in blood, genital, rectal compartments was determined HIV-positive seronegative adults who undertook a 60-day intensive PK study daily TDF/FTC (plus efavirenz HIV positives). Lymphocyte cell sorting, sampling occurred once per subject, at staggered visits. Among 19 (3 female) 21 (10 adults, TFV-DP peripheral blood...
Abstract Background Although tenofovir diphosphate (TFV-DP) in dried blood spots (DBS) is a predictor of adherence and pre-exposure prophylaxis efficacy, its utility human immunodeficiency virus (HIV) treatment remains unknown. Methods DBS for TFV-DP were collected up to 3 times over 48 weeks persons living with HIV (PLWH) who receiving TFV disoproxil fumarate (TDF)-based therapy. Log-transformed baseline was compared using t-tests or analyses variance; generalized estimating equations used...
ABSTRACT New objective measures of antiretroviral adherence are needed. We determined if emtricitabine triphosphate (FTC-TP) in dried blood spots (DBS) can be used as a marker recent dosing with tenofovir disoproxil fumarate-emtricitabine (TDF-FTC). The half-life FTC-TP was estimated DBS samples obtained from an intensive pharmacokinetic (PK) study coformulated TDF-FTC HIV-negative and HIV-infected participants. concordance quantifiable (TFV)/FTC plasma evaluated by utilizing paired...
Background. This study estimated the number of daily tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) doses required to achieve and maintain (after discontinuation) intracellular drug concentrations that protect against human immunodeficiency virus (HIV) infection for men who have sex with (MSM). Methods. Tenofovir diphosphate (TFV-DP) in peripheral blood mononuclear cells (PBMCs) rectal from an intensive pharmacokinetic ("Cell-PrEP" [preexposure prophylaxis]) 30 days TDF/FTC followed...
Abstract Background Tenofovir diphosphate (TFV-DP) in dried blood spots (DBS) is associated with viral suppression persons living HIV (PLWH) taking tenofovir disoproxil fumarate (TDF). However, its value as a predictor of future viremia remained unknown. Methods Blood for plasma load (VL) and TFV-DP DBS were collected (up to 3 visits within 48 weeks) PLWH on TDF. cut points selected using logistic prediction models maximizing the area under receiver operation characteristic curve, estimated...
Macroautophagy (autophagy) utilizes a serial of receptors to specifically recognize and degrade autophagy cargoes, including damaged organelles, maintain cellular homeostasis. Upstream signals spatiotemporally regulate the biological functions selective through protein post-translational modifications (PTM) such as phosphorylation. However, it is unclear how acetylation directly controls in autophagy. Here, we report that an ER-phagy receptor FAM134B acetylated by CBP acetyltransferase,...
Simple and reproducible tools to assess antiretroviral adherence are needed. A level of tenofovir diphosphate (TFV-DP) in dried blood spots (DBS) <1,250 fmol/punch is predicted identify imperfect adherence. Herein we evaluated TFV-DP DBS as a measure among HIV-infected women. peripheral mononuclear cells (PBMCs) were collected twice (∼1 week apart) 35 well-controlled women [median age 42 years, 14 African American/black (AA)] receiving daily coformulated tenofovir/emtricitabine either...
Abstract Polycystic ovary syndrome (PCOS) is a complex endocrine disorder and regarded as the leading cause of anovulatory infertility. PCOS characterized by reproductive dysfunction metabolic disorders. Baicalin (BAL) one most potent bioactive flavonoids isolated from radix Scutellaria baicalensis. In present study, we investigated potential effects BAL on in dehydroepiandrosterone-treated rats. We found that notably reduced serum levels free testosterone, total follicle-stimulating...
Previous studies have primarily focused on nucleos(t)ide reverse transcriptase inhibitor pharmacology in peripheral blood mononuclear cells (PBMCs) and erythrocytes via dried spots (DBS), but not other major cells. Our objectives were to describe compare the concentrations of tenofovir-diphosphate (TFV-DP) emtricitabine-triphosphate (FTC-TP) DBS, PBMCs, neutrophils, platelets people with HIV (PWH) without (PWOH). isolated from whole drawn PWH PWOH receiving tenofovir alafenamide...
Abstract Background QUANTI-TAF aimed to establish tenofovir-diphosphate (TFV-DP)/emtricitabine-triphosphate (FTC-TP) adherence benchmarks in dried blood spots (DBS) for persons with human immunodeficiency virus (PWH) receiving tenofovir alafenamide/emtricitabine (TAF/FTC)–based antiretroviral therapy (ART). Methods For 16 weeks, PWH received TAF/FTC-based ART co-encapsulated an ingestible sensor directly measure cumulative (enrollment final visit) and 10-day adherence. At monthly visits,...
The drivers of low-level viremia (LLV) between 20 and 200 copies/mL remain unclear. In 1042 person-visits from 497 persons with HIV on tenofovir disoproxil fumarate-containing antiretroviral therapy (ART), the association LLV cumulative adherence (quantified using diphosphate [TFV-DP] in dried blood spots) was assessed. Lower TFV-DP levels were associated higher odds LLV. As (fmol/punch) categories decreased >1650 to 800-1650; 800-1650 <800; <800, adjusted ratios for vs VL <20 2.0 (95% CI,...
Elevated immune activation is associated with an increased risk of HIV acquisition. Tenofovir (TFV) has immunomodulatory properties in vitro, but how this extends vivo remains unknown.HIV-negative adults received daily coformulated TFV disoproxil fumarate 300 mg/emtricitabine (FTC) 200 mg for 30 days followed by a 30-day washout. Markers T-cell activation, inflammation, and cytokines were measured before drug on (on drug) 60 (30-day washout). Data analyzed using one-way analysis...
Glasses with compositions 50Bi 2 O 3 – x Sb –10B –(40– ) SiO ( =0, 1, 3, 5, 8, 10) have been prepared by conventional melt quench technique. Substitution of for exerted an obvious effect on properties glasses, especially, increased glass transition temperature T g and crystalline c greatly. Results infrared transmission spectra attributed the to formation new bridging bonds Sb–O–B Sb–O–Si in network.
Concentrations of tenofovir (TFV) in hair and diphosphate (TFV-DP) dried blood spots (DBSs) as measures cumulative exposure have been primarily studied younger, HIV-uninfected individuals taking preexposure HIV prophylaxis. Data on these among older HIV-infected are limited.We evaluated longitudinal TFV TFV-DP concentrations DBS, respectively, from adults. Multivariable model variables included age group (18-35 60 years older), creatinine clearance (CrCl), hematocrit (TFV-DP), gray color...
Tenofovir diphosphate (TFV-DP) in dried blood spots (DBS) is a strong predictor of viral suppression persons living with HIV (PLWH). Its association antiretroviral therapy (ART) resistance remains unknown.Blood was collected PLWH receiving TDF-containing ART enrolled 48-week study. diphosphate/emtricitabine triphosphate (FTC-TP) were quantified from the same sample as load (VL) who developed within ≤12 months.The study 807 participants, whom 10 had new resistance-conferring mutations. Among...
To determine factors associated with interindividual variability in tenofovir diphosphate (TFV-DP) concentrations dried blood spots (DBSs) among persons living HIV (PLWH).PLWH who were at least 18 years old and taking disoproxil fumarate-containing ART prospectively recruited enrolled from a clinical cohort followed longitudinally (up to three visits over 48 weeks). With log-transformed TFV-DP DBSs as the outcome, mixed-model regression analyses used assess associations between self-reported...