M Jahangir Hossain
A5101512539- Viral gastroenteritis research and epidemiology
- Child Nutrition and Water Access
- Escherichia coli research studies
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Parasitic Infections and Diagnostics
- Identification and Quantification in Food
- Global Maternal and Child Health
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Food Security and Health in Diverse Populations
- COVID-19 epidemiological studies
- Fecal contamination and water quality
- Gut microbiota and health
- Vaccine Coverage and Hesitancy
- Poverty, Education, and Child Welfare
- Plant Virus Research Studies
- Respiratory viral infections research
- Bacterial biofilms and quorum sensing
- Cultural Competency in Health Care
MRC Unit the Gambia
2016-2024
Medical Research Council
2013-2024
London School of Hygiene & Tropical Medicine
2024
Auburn University
2008-2019
International Centre for Diarrhoeal Disease Research
2010
Shigella case isolates from the Global Enteric Multicenter Study were serotyped to guide vaccine development. A quadrivalent that includes O antigens S. sonnei, flexneri 2a, 3a, and 6 should provide broad protection. Background. Shigella, a major diarrheal disease pathogen worldwide, is target of The (GEMS) investigated burden etiology moderate-to-severe in children aged <60 months matched controls without diarrhea during 3 years at 4 sites Africa Asia. was 1 most common pathogens across age...
Background The importance of Cryptosporidium as a pediatric enteropathogen in developing countries is recognized. Methods Data from the Global Enteric Multicenter Study (GEMS), 3-year, 7-site, case-control study moderate-to-severe diarrhea (MSD) and GEMS-1A (1-year MSD less-severe [LSD]) were analyzed. Stools 12,110 3,174 LSD cases among children aged <60 months 21,527 randomly-selected controls matched by age, sex community immunoassay-tested for Cryptosporidium. Species subset...
Diarrheal diseases remain a leading cause of illness and death among children younger than 5 years in low-income middle-income countries. The Global Enteric Multicenter Study (GEMS) has described the incidence, aetiology, sequelae medically attended moderate-to-severe diarrhoea (MSD) aged 0-59 months residing censused populations sub-Saharan Africa south Asia, where most child deaths occur. To further characterise this disease burden guide interventions, we extended study to include with...
The Global Enteric Multicenter Study (GEMS) was a 3-year case-control study that measured the burden, aetiology, and consequences of moderate-to-severe diarrhoea (MSD) in children aged 0-59 months. GEMS-1A, 12-month follow-on study, comprised two parallel studies, one assessing MSD other less-severe (LSD). In this report, we analyse risk death with each type specific pathogens associated fatal outcomes.GEMS prospective, age-stratified, matched done at seven sites Africa Asia. Children months...
ABSTRACT Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile septicemia (MAS) catfish. The possible origin this newly emerged A. strain is unknown. In study, we show using whole-genome sequencing comparative genomics that isolates from diseased grass carp China have similar genomes. Our phylogenomic analyses suggest U.S. populations Asian origin. Furthermore, identified an...
Lineages of hypervirulent Aeromonas hydrophila (vAh) are the cause persistent outbreaks motile septicemia in warm-water fishes worldwide. Over last decade, People's Republic China and United States, this virulent lineage A. has resulted annual losses millions tons farmed carp catfish. Multiple lines evidence indicate that US catfish Asian isolates affiliated with sequence type 251 (ST251) share a recent common ancestor. To address genomic context for putative intercontinental transfer...
The association between childhood diarrheal disease and linear growth faltering in developing countries is well described. However, the impact attributed to specific pathogens has not been elucidated, nor of recommended antibiotic treatment.The Global Enteric Multicenter Study enrolled children with moderate severe diarrhea (MSD) seeking healthcare at 7 sites sub-Saharan Africa South Asia. At enrollment, we collected stool samples identify enteropathogens. Length/height was measured...
Abstract Background We evaluated the burden of Shigella spp from children aged 0–59 months with medically attended moderate-to-severe diarrhea and matched controls at sites in Mali, The Gambia, Kenya participating Vaccine Impact on Diarrhea Africa (VIDA) study 2015 to 2018. Methods were identified using coprocultures serotyping addition quantitative polymerase chain reaction (qPCR). Episode-specific attributable fractions (AFe) for calculated DNA quantity; cases AFe ≥0.5 considered have...
Shigella is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting are development, and phase 3 clinical trials imminent to determine efficacy against shigellosis. The Enterics for Global Health (EFGH) surveillance study designed the incidence medically attended shigellosis 6- 35-month-old 7 resource-limited settings. Here, we describe microbiological methods used isolate identify Shigella. We developed standardized laboratory protocol isolation identification by...
To investigate the molecular basis of emergence Aeromonas hydrophila responsible for an epidemic outbreak motile aeromonad septicemia catfish in Southeastern United States, we sequenced 11 A. isolates that includes five reference and six recent isolates. Comparative genomics revealed are highly clonal, whereas greatly diverse. We identified 55 epidemic-associated genetic regions with 313 predicted genes present but absent from 35% these located within genomic islands, suggesting their...
Abstract Background Diarrheal disease is heterogeneous, including watery diarrhea (WD) and dysentery, some cases of which become persistent (PD). Changes in risk over time necessitate updated knowledge these syndromes sub-Saharan Africa. Methods The Vaccine Impact on Diarrhea Africa (VIDA) study was an age-stratified, case-control moderate-to-severe among children &lt;5 years old Gambia, Mali, Kenya (2015–2018). We analyzed with follow-up about 60 days after enrollment to detect PD...
Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella clinical samples compared culture-based methods, which underestimate burden by 2- 3-fold. qPCR assays have also developed for speciation and serotyping, is critical both vaccine development evaluation. The Enterics Global Health (EFGH) surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, the detection...
To understand the growth-promoting and disease-inhibiting activities of plant rhizobacteria (PGPR) strains, genomes 12 Bacillus subtilis group strains with PGPR activity were sequenced analyzed. These B. exhibited high genomic diversity, whereas amyloliquefaciens (a member group) are highly conserved. A pairwise BLASTp matrix revealed that gene family similarity among ranges from 32 to 90%, 2839 genes within core genome subsp. plantarum. Comparative analyses identified linked biological...
Abstract Diarrheal disease, still a major cause of childhood illness, is caused by numerous, diverse infectious microorganisms, which are differentially sensitive to environmental conditions. Enteropathogen‐specific impacts climate remain underexplored. Results from 15 studies that diagnosed enteropathogens in 64,788 stool samples 20,760 children 19 countries were combined. Infection status for 10 common enteropathogens—adenovirus, astrovirus, norovirus, rotavirus, sapovirus, Campylobacter ,...
Abstract Background Stunting affects &gt;20% of children &lt;5 years old worldwide and disproportionately impacts underserved communities. The Vaccine Impact on Diarrhea in Africa (VIDA) Study examined the association between an episode moderate-to-severe diarrhea (MSD) risk subsequent stunting living 3 sub-Saharan African countries. Methods In this prospective, matched, case-control study among years, data were collected over 36 months from 2 groups. “Children with MSD” visited a...
Aeromonas hydrophila is a Gram-negative, rod-shaped, mesophilic bacterium that infects both aquatic poikilothermic animals and mammals, including humans. Here, we present the complete genome sequence of strain ML09-119, which represents clonal group A. isolates causing outbreaks bacterial septicemia in channel catfish since 2009.
Four Aeromonas strains from clinical and environmental samples differed known species on the basis of rpoD gene sequence. Multilocus phylogenetic analysis in silico DNA-DNA hybridization confirmed them as four new even though their 16S rRNA sequence similarity with closest relatives was >98.7%, occurred for other spp.
Sick newborns admitted to neonatal units in low-resource settings are at an increased risk of developing hospital-acquired infections due poor clinical care practices. Clusters infection, the same species, with a consistent antibiotic resistance profile, and ward over short period time might be indicative outbreak. We used whole-genome sequencing (WGS) define transmission pathways characterise two distinct outbreaks bacteraemia west African unit.We studied Burkholderia cepacia...
Abstract Background Despite antibiotic prescription being recommended for dysentery and suspected cholera only, diarrhea still triggers unwarranted prescription. We evaluated antibiotic-prescribing practices their predictors among children aged 2–59 months in the Vaccine Impact on Diarrhea Africa (VIDA) Study performed The Gambia, Mali, Kenya. Methods VIDA was a prospective case-control study (May 2015–July 2018) presenting care with moderate-to-severe (MSD). defined inappropriate use as or...
Rigorous data management systems and planning are essential to successful research projects, especially for large, multicountry consortium studies involving partnerships across multiple institutions. Here we describe the development implementation of procedures Enterics For Global Health (EFGH) Shigella surveillance study-a 7-country diarrhea study that will conduct facility-based concurrent with population-based enumeration a health care utilization survey estimate incidence...
Accurate estimation of diarrhea incidence from facility-based surveillance requires estimating the population at risk and accounting for case patients who do not seek care. The Enterics Global Health (EFGH) Shigella study will characterize denominators healthcare-seeking behavior proportions to calculate rates in children aged 6-35 months across 7 sites Africa, Asia, Latin America. use a hybrid design, supplementing with population-based surveys estimate size proportion brought care EFGH...
Molecular diagnostics on human fecal samples have identified a larger burden of shigellosis than previously appreciated by culture. Evidence fold changes in immunoglobulin G (IgG) to conserved and type-specific Shigella antigens could be used validate the molecular assignment as etiology acute diarrhea support polymerase chain reaction (PCR)-based microbiologic end points for vaccine trials. We will test dried blood spots collected at enrollment 4 weeks later using bead-based immunoassays...
We report the clinical findings, epidemiology, and risk factors for moderate-to-severe diarrhea (MSD) associated with Aeromonas species in children 0-59 months of age, from Global Enteric Multicenter Study, conducted at three sites south Asia four sub-Saharan Africa. Children MSD were enrolled along controls matched gender, neighborhood. Pooled, age-stratified conditional logistic regression models applied to evaluate association infection controlling coinfecting pathogens sociodemographic...