A5070753697- Escherichia coli research studies
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Clostridium difficile and Clostridium perfringens research
- Aquaculture disease management and microbiota
- Vaccine Coverage and Hesitancy
- Child Nutrition and Water Access
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Bacterial Genetics and Biotechnology
- Diabetes and associated disorders
- Salmonella and Campylobacter epidemiology
- Hepatitis B Virus Studies
- Transgenic Plants and Applications
- Protein purification and stability
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
University of Gothenburg
2015-2025
The University of Texas Southwestern Medical Center
1974-2015
Institute for Biomedicine
2007-2010
Karolinska Institutet
1969-2010
Karolinska University Hospital
2010
Institut de Recherche Vaccinale
2009
International Centre for Diarrhoeal Disease Research
1984-2000
Svettmottagningen
1999
Bipar
1999
Trelleborg (Sweden)
1998
By a double-diffusion precipitation-in-gel technique, isolated cholera toxin as well its natural toxoid were shown to be fixed and precipitated by the ganglioside G M1 but not any of related glycolipids M3 , M2 -GlcNAc, D1a D1b T1 globoside, A1 tetrahexoside-GlcNAc. Twenty-five nanograms was enough give precipitation line with 1.2 μg toxin, whereas about 50 ng required this amount toxoid. also inactivated in ileal loop intradermal models rabbits. A 1: 1 molar ratio found limiting, e.g., 100...
Ganglioside GM1 was isolated from the small intestinal mucosa of man, pig, and beef amounted to 0.1, 2.0, 43 nmol per g fresh weight, respectively. These differences in content were associated with a quantitatively differing ability mucosal cells bind cholera toxin. Human bound about 15,000 toxin molecules when saturated toxin, porcine 120,000, bovine 2,600,000 molecules. The association constant (KA) binding was, for all three species, 10(9) liters/mol. Exogenously added ganglioside...
We performed a prospective study to examine whether the IgA antibodies against cholera that are present in breast milk protect breast-fed infants and children colonization with Vibrio cholerae 01 disease. Among families of patients cholera, we collected from mothers who had not diarrhea previous week monitored them their for 10 days. Breast was assayed toxin lipopolysaccharide. Ninety-three mother--child pairs were studied; 30 became colonized V. disease developed 19. There no differences...
An immunoelectron microscopic method is described for sensitive high-resolution visualization of tissuebound cholera toxin. The principle to incubate cells or tissue sections with toxin and then localize the bound toxin-specific peroxidase (donor:hydrogen-peroxide oxidoreductase; EC 1.11.1.7)-conjugated antibody enzyme substrate. Thin are examined electron-opaque precipitates in a transmission electron microscope. Because specific binding membrane ganglioside G(M1), can be used...
The in vitro binding properties of enterotoxins Vibrio cholerae and Escherichia coli to different pure gangliosides related neutral glycosphin-golipids were analyzed with a sorbent assay utilizing plastic tubes which the glycolipid substances had been coupled. It was found that cholera toxin bound G M1 ganglioside better than other tested M3 , -NGN, M2 D1a D1b T A1 tetrahexoside-GlcNac globoside. With this using -coated it is possible measure even at concentrations below 1 ng/ml. Also...
Oral administration of antigens, including allergens and autoantigens, may be an efficient way to prevent diseases associated with untoward immune responses self- non-self-antigens. However, this approach has met limitations because it usually requires repeated administrations large doses antigen is less in already host, the effect short duration. We report that a single oral minute amounts particulate or soluble coupled B subunit cholera toxin (CTB) can markedly suppress systemic naive...
Abstract This study addressed the question of whether mucosal adjuvant property cholera toxin (CT) and structurally closely related Escherichia coli heat‐labile (LT) requires enterotoxic adenylate cyclase/cAMP activating these molecules. Therefore, we investigated cytotoxic abilities enterotoxins compared results with those obtained non‐toxic CT LT derivatives; recombinant CTB (rCTB) a mutated (mLT), which had single amino acid substitution in position 112 (Glu→Lys) A subunit. Detailed...
At the Matlab Hospital of international Centre for Diarrhoeal Disease Research, Bangladesh, authors examined blood groups patients hospitalized between January and September 1979 diarrheal disease due to a variety bacterial viral agents. A significant association was identified only cholera, in which cholera were twice as likely have group O one-ninth AB community controls. follow-up study family contacts patients, carried out 1980 July 1982, indicated that did not affect an individual's...
Forty-five volunteers were vaccinated twice intranasally with 10, 100, or 1,000 microg of cholera toxin B subunit (CTB). Blood and nasal vaginal secretions collected before 1 week after the second vaccination from all volunteers, specific total immunoglobulin A (IgA) IgG titers determined by enzyme-linked immunosorbent assay. Samples also taken 6 months (n = 16) year 14) vaccination. The 10- 100-microg doses well tolerated but 1,000-microg dose induced increased nose repetitive sneezings for...
Generation of local and systemic immune responses by the oral administration antigens is frequently inefficient, requiring large quantities immunogens yielding only modest antibody responses. In this study, we have demonstrated that microgram amounts Streptococcus mutans protein antigen I/II covalently coupled to B subunit cholera toxin elicits vigorous mucosal as well extramucosal immunoglobulin A G antistreptococcal in mice. These were manifested presence numbers antibody-secreting cells...
ABSTRACT We determined the types of cholera toxin (CT) produced by a collection 185 Vibrio cholerae O1 strains isolated in Bangladesh over past 45 years. All El Tor V. since 2001 CT classical biotype, while those before biotype.
Journal Article Mucosal Antitoxic and Antibacterial Immunity after Cholera Disease Immunization with a Combined B Subunit-Whole Cell Vaccine Get access Ann-Mari Svennerholm, Svennerholm Search for other works by this author on: Oxford Academic PubMed Google Scholar Marianne Jertborn, Jertborn I.eif Gothefors, Gothefors A. M. Karim, Karim David Sack, Sack Jan Holmgren The of Infectious Diseases, Volume 149, Issue 6, June 1984, Pages 884–993, https://doi.org/10.1093/infdis/149.6.884 Published:...