A5069530144- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Carcinogens and Genotoxicity Assessment
- Indoor Air Quality and Microbial Exposure
- Air Quality and Health Impacts
- Insect symbiosis and bacterial influences
- Microbial Metabolism and Applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Effects and risks of endocrine disrupting chemicals
- Fungal and yeast genetics research
- Toxic Organic Pollutants Impact
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Insect Pheromone Research and Control
- Building materials and conservation
- Diverse Education Studies and Reforms
- Analytical Chemistry and Chromatography
- Phytochemical compounds biological activities
- Cocoa and Sweet Potato Agronomy
- Autophagy in Disease and Therapy
University of Bristol
2011-2024
University of the West of England
2024
Massachusetts General Hospital
2024
Harvard University
2024
University of Southampton
2021
Leibniz University Hannover
2016-2019
Schneider Electric (France)
2017
At Bristol
2014
Carroll College
2012
NCCOS Hollings Marine Laboratory
2010
Genome mining of the fungus Mucor irregularis (formerly known as Rhizomucor variabilis) revealed presence various gene clusters for secondary metabolite biosynthesis, including several terpene-based clusters. Investigation into chemical diversity M. QEN-189, an endophytic isolated from fresh inner tissue marine mangrove plant Rhizophora stylosa, resulted in discovery 20 structurally diverse indole-diterpenes six new compounds, namely, rhizovarins A–F (1–6). Among them, compounds 1–3...
Late stage oxidations during the biosynthesis of 2-pyridone tenellin in insect pathogenic fungus Beauveria bassiana were investigated by a combination gene knockout, antisense RNA, and coexpression studies. Open reading frames (ORF) 3 4 biosynthetic cluster previously shown to encode trans-acting enoyl reductase hybrid polyketide synthase nonribosomal peptide synthetase (PKS-NRPS), respectively, which together synthesize acyltetramic acid pretenellin-A. In this work, we have that ORF1...
Trypanosoma brucei undergoes genetic exchange in its insect vector, the tsetse fly, by an unknown mechanism. The difficulties of working with this experimental system have hampered investigation, particularly because trypanosome life cycle stages involved cannot be cultured vitro and therefore must examined insect. Searching for small numbers hybrid trypanosomes directly fly has become possible through incorporation fluorescent reporter genes, we previously carried out a successful cross...
ADVERTISEMENT RETURN TO ISSUEPREVArticleIndoor air sampling and mutagenicity studies of emissions from unvented coal combustionJudy L. Mumford, D. Bruce. Harris, Katherine. Williams, Jane C. Chuang, Marcus. CookeCite this: Environ. Sci. Technol. 1987, 21, 3, 308–311Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/es00157a014https://doi.org/10.1021/es00157a014research-articleACS...
A novel polyketide synthase nonribosomal peptide synthetase (PKS-NRPS) genecluster was isolated from Beauveria bassiania 992.05. The cluster encodes the enzymes responsible for biosynthesis of new 2-pyridone desmethylbassianin (DMB). DMB is structurally related to tenellin B. bassiana 110.25 but it differs in chain length and degree methylation. Despite these programming differences 20 kb biosynthetic has 90% sequence identity tenellingenecluster. Silencing PKS-NRPS gene, dmbS, resulted...
Abstract Fungal maleidrides are an important family of bioactive secondary metabolites that consist 7, 8, or 9‐membered carbocycles with one two fused maleic anhydride moieties. The biosynthesis byssochlamic acid (a nonadride) and agnestadride A heptadride) was investigated through gene disruption heterologous expression experiments. results reveal the precursors for cyclization formed by iterative highly reducing fungal polyketide synthase supported a hydrolase, together citrate‐processing...
Identification of a reductase (AgnL4) confirms that<italic>in vivo</italic>anthraquinone to anthrol conversion is an essential first step in aromatic deoxygenation anthraquinones catalysed by AgnL6 (reductase) and AgnL8 (dehydratase).
Exchange of 32 different sub-fragments the C-methyltransferase (C-MeT), pseudo-ketoreductase (ΨKR) and ketoreductase (KR) catalytic domains tenellin iterative Type I polyketide synthase non ribosomal peptide synthetase (PKS-NRPS) TENS by homologous fragments from desmethylbassianin (DMBS) militarinone (MILS) PKS-NRPS led to creation chimeric synthetases in which programming fidelity was altered, resulting production mixtures products with methylation patterns chain lengths. Swap KR domain...
Abstract Burkholderia have potential as biocontrol agents because they encode diverse biosynthetic gene clusters (BGCs) for a range of antimicrobial metabolites. Given the opportunistic pathogenicity associated with species, heterologous BGC expression within non‐pathogenic hosts is strategy to construct safe strains. We constructed yeast‐adapted Burkholderia‐Escherichia shuttle vector (pMLBAD_yeast) yeast replication origin 2 μ and URA3 selection marker optimised it cloning BGCs using in...
Byssochlamic acid and the novel heptadride, agnestadride A are biosynthesised <italic>via</italic> alternative dimerisations of a maleic monomer.
Human exposure to polycyclic aromatic hydrocarbons (PAHs) has been determined by measurement of DNA adducts in human tissues. Competitive enzyme-linked immunosorbent assays (ELISAs) using antisera recognizing benzo[a]pyrenediol-epoxide-modified (BPDE-I-DNA) and color fluorescence endpoint detection have used extensively for quantifying PAH-DNA adducts. The ELISA (limit 1 adduct/10(8) nucleotides) was previously reported be more sensitive than the (1/10(7)) measuring PAH (Santella et al....
Trypanosoma brucei undergoes genetic exchange in its insect vector, but the mechanism is unknown and no one has yet seen process. By crossing genetically engineered red green fluorescent trypanosomes, we have been able to pinpoint location of fly search for intermediate stages. In experimental crosses parental yellow hybrid trypanosomes first appeared salivary glands as early 13 days after infection were observed only flies with a mixture or both glands. Despite high numbers mixed...
The biosynthesis of the herbicide cornexistin in fungus Paecilomyces variotii was investigated by full sequencing its genome, knockout key genes within biosynthetic gene cluster and isolation identification intermediate compounds. general pathway resembles that byssochlamic acid other nonadrides early stages, but differs requiring fewer enzymes nonadride dimerisation step, removal one maleic anhydride moiety.
Abstract Background Microscopic colitis (MC) is a common cause of chronic diarrhea, predominantly among older adults. Emerging evidence suggests that perturbations gut microbiome and metabolome may play an important role in MC pathogenesis. Objective To comprehensively characterize alterations the microbial metabolic composition MC. Design We established longitudinal cohort adult patients with two control groups individuals – diarrhea controls age- sex-matched without diarrhea. Using stool...
We have performed bioassay-directed fractionation of a model complex mixture (rabbit lung S9-generated metabolites 14C-radiolabeled 1-nitropyrene) by assaying reverse-phase HPLC fractions using two microsuspension mutagenicity assays. A forward-mutation assay measuring mutation at the gpt locus (8-azaguanine resistance) in Salmonella typhimurium TM677 was total volume 100 microliters, and reverse-mutation hisD3052 allele S. TA98 200 microliters. were collected every 30 s for 45 min,...
Naematolin is a biologically active sesquiterpene produced by Hypholoma species. Low titres and complex structure constrain the exploitation of this secondary metabolite. Here, we de novo sequenced H. fasciculare genome to identify candidate biosynthetic gene cluster for production naematolin. Using Aspergillus oryzae as heterologous host expression, activity several synthases were investigated, highlighting one atypical synthase apparently capable catalysing 1,11 subsequent 2,10 ring...
Abstract Die von Pilzen produzierten Maleidride sind eine wichtige Klasse bioaktiver Sekundärmetaboliten, die aus sieben‐, acht‐ oder neungliedrigen Carbocyclen bestehen. Jeder Cyclus ist mit einem zwei anellierten Maleinsäureanhydridresten substituiert. Durch Gen‐Knockouts und heterologe Expressionsexperimente wurde Biosynthese Byssochlaminsäure (ein Nonadrid) Agnestadrid A Heptadrid) untersucht. Unsere Experimente zeigen, dass der Vorläufer Cyclisierung einer iterativen, vollständig...
Four environmental emissions samples were ranked by their genotoxic potency in several bioassays. Although the relative of a series automotive (diesel and gasoline) Ames assay correlated well with mammalian cell mouse skin, this was not case for coke oven, roofing tar, cigarette smoke condensate (CSC) emissions. This study examines role metabolic activation determining difference between microbial bioassay ranking these Uninduced Aroclor 1254-induced S9 from both rat hamster liver compared...