A5085282424- Biofuel production and bioconversion
- Animal Virus Infections Studies
- Plant Gene Expression Analysis
- Viral gastroenteritis research and epidemiology
- Lignin and Wood Chemistry
- Virus-based gene therapy research
- Biochemical and biochemical processes
- Bioenergy crop production and management
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Genetically Modified Organisms Research
- Influenza Virus Research Studies
- Animal Genetics and Reproduction
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Vector-Borne Animal Diseases
- Photosynthetic Processes and Mechanisms
- Cytomegalovirus and herpesvirus research
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Microbial Metabolic Engineering and Bioproduction
Katholieke Hogeschool Vives
2014-2025
Veterinary and Agrochemical Research Centre
2022
Catholic University College of Bruges–Ostend
2007-2014
University of Wisconsin–Madison
2000-2009
University of Guelph
2009
Ghent University Hospital
2009
Vlaams Instituut voor Biotechnologie
1996-2004
Ghent University
1991-2004
VIB-UGent Center for Plant Systems Biology
2001-2004
Institut National de la Recherche Agronomique
2002
The first enzyme of the phenylpropanoid pathway, Phe ammonia-lyase (PAL), is encoded by four genes in Arabidopsis thaliana. Whereas PAL function well established various plants, an insight into functional significance individual gene family members lacking. We show that absence clear phenotypic alterations pal1 and pal2 single mutants with limited double mutant, significant modifications occur transcriptome metabolome pal mutants. disruption led to transcriptomic adaptation components...
Abstract Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in biosynthesis of lignin precursors, monolignols. We have down-regulated CAD transgenic poplar (Populus tremula X Populus alba) by both antisense and co-suppression strategies. Several sense poplars had an approximately 70% reduced activity that was associated with a red coloration xylem tissue. Neither amount nor monomeric composition (syringyl/guaiacyl) were significantly modified. However, phloroglucinol-HCl staining...
Summary Cinnamoyl CoA:NADP oxidoreductase (CCR, EC 1.2.1.44) catalyzes the conversion of cinnamoyl CoA esters to their corresponding cinnamaldehydes, i.e. first specific step in synthesis lignin monomers. The cloning a cDNA encoding CCR Eucalyptus gunnii (EUCCR) is reported here. identity EUCCR was demonstrated by comparison with peptide sequence data from purified and functional expression recombinant enzyme Escherichia coli . Sequence analysis revealed remarkable homologies...
Summary Lignin is a polymeric constituent of the cell wall that needs to be removed during paper making process. Bi‐specific caffeic acid/5‐hydroxyferulic acid O ‐methyltransferase (COMT) catalyses ‐methylation and 5‐hydroxyferulic ferulic sinapic acid, respectively. These compounds are intermediates in biosynthesis lignin precursors. Therefore, COMTs potential target enzymes for reducing amount, or modifying composition, plants. Different antisense sense constructs have been expressed gene...
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) is divided into a European North American genotype. East PRRSV isolates have been found to be of the genotype, but form different subtypes. In present study, was isolated from Belarusian farm with failure designated "Lena". Analyses revealed that Lena new subtype 3 isolate. The main purpose this investigation study pathogenesis antigenic characteristics (Lena). Results Obvious clinical virological differences...
Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis Arabidopsis (Arabidopsis thaliana) and later Medicago truncatula. However, the general function of this enzyme questioned by apparent lack CSE activity lignifying tissues different plant species. Here, we show that down-regulation hybrid poplar (Populus tremula × Populus alba) resulted up 25% reduced deposition, increased levels p-hydroxyphenyl units polymer, a relatively higher cellulose...
Summary Precise gene‐editing methods are valuable tools to enhance genetic traits. Gene editing is commonly achieved via stable integration of a cassette in the plant's genome. However, this technique unfavorable for field applications, especially vegetatively propagated plants, such as many commercial tree species, where cannot be segregated away without breaking constitution elite variety. Here, we describe an efficient method generating gene‐edited Populus tremula × P. alba (poplar) trees...
Scavenger receptor CD163 is a key entry mediator for porcine reproductive and respiratory syndrome virus (PRRSV). To identify the protein domains involved in PRRSV infection, deletion mutants chimeric were created. Infection experiments revealed that scavenger cysteine-rich (SRCR) domain 5 (SRCR 5) essential while four N-terminal SRCR cytoplasmic tail are not required. The remaining need to be present but can replaced by corresponding from CD163-L1, resulting reduced 6 interdomain regions)...
Abstract Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2 (−/*) line 12 poplars one knockout allele while the other contains a 3-bp deletion, resulting 114I115A-to-114T conversion corresponding protein. Despite having 10% less lignin, grows normally. On plant basis, saccharification efficiency of is...
Lignins are cell wall-located aromatic polymers that provide strength and hydrophobicity to woody tissues. Lignin monomers synthesized via the phenylpropanoid pathway, wherein CAFFEOYL SHIKIMATE ESTERASE (CSE) converts caffeoyl shikimate into caffeic acid. Here, we explored role of two CSE homologs in poplar (Populus tremula × P. alba). Reporter lines showed expression conferred by both CSE1 CSE2 promoters is similar. CRISPR-Cas9-generated cse1 cse2 single mutants had a wild-type lignin...
Cinnamoyl-CoA reductase (CCR) catalyses the first specific step in biosynthesis of monolignols, monomeric units lignins. We examined developmental regulation Eucalyptus gunnii EgCCR promoter by analysing expression EgCCR-GUS fusions tobacco. activity was strongest lignified organs (stems and roots) consistent with mRNA level these organs. Histochemical analysis showed vascular tissues (cambium, young differentiating xylem, ray cells, internal external phloem) stems roots agreement situ...
Benzodioxane structures are produced in lignins of transgenic poplar plants deficient COMT, an O-methyltransferase required to produce lignin syringyl units.They result from incorporation 5-hydroxyconiferyl alcohol into the monomer supply and confirm that phenols other than three traditional monolignols can be integrated plant lignins.
This study examined whether antigenic differences among porcine circovirus type 2 (PCV-2) strains could be detected using monoclonal antibodies (mAbs). A subtractive immunization protocol was used for the genotype post-weaning multisystemic wasting syndrome (PMWS)-associated PCV-2 strain Stoon-1010. Sixteen stable hybridomas that produced mAbs with an immunoperoxidase monolayer assay (IPMA) titre of 1000 or more to Stoon-1010 were obtained. Staining recombinant virus-like particles...
The porcine reproductive and respiratory syndrome virus (PRRSV) is a rapidly evolving pathogen of swine. At present, there high demand for safe more effective vaccines that can be adapted regularly to emerging variants. A recent study showed that, by the use controlled inactivation procedure, an experimental BEI-inactivated PRRSV vaccine developed offers partial protection against homologous challenge with prototype strain LV. it however not known if this currently circulating In study, two...
The first step in the biosynthetic pathway of vitamin C plants is formation, at level sugar nucleotide, l -galactosyl residues, catalyzed by a largely unknown GDP- d -mannose 3",5"-epimerase. By using combined conventional biochemical and mass spectrometry methods, we obtained highly purified preparation 3",5"-epimerase from an Arabidopsis thaliana cell suspension. native enzyme 84-kDa dimer, composed two apparently identical subunits. In-gel tryptic digestion subunit, followed peptide...
Porcine reproductive and respiratory syndrome virus (PRRSV) can induce severe failure in sows, is involved the porcine disease complex. The glycoprotein GP4 of European prototype PRRSV strain Lelystad (LV) contains a linear neutralizing epitope that located highly variable region. current study aimed to evaluate antibody response against this other epitopes on infection pigs with European-type PRRSV. It was shown three strains, differing region corresponds LV, strongly antibodies area....
We tested serum samples from pigs infected or vaccinated with European swine influenza viruses (SIVs) in hemagglutination-inhibition assays against pandemic (H1N1) 2009 virus and related North American SIVs. found more serologic cross-reaction than expected. Data suggest Europe may have partial immunity to virus.
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo , infects a subpopulation of tissue macrophages. vitro PRRSV only replicates primary macrophages African green monkey kidney derived cells, such as Marc-145. The latter is currently used for vaccine production. However, since entry Marc-145 cells different compared to macrophages, specific epitopes associated with could potentially alter upon...