A5044314722- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Bioeconomy and Sustainability Development
- Cell Image Analysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Atmospheric chemistry and aerosols
- Nematode management and characterization studies
- Antifungal resistance and susceptibility
- Plant Pathogens and Resistance
- nanoparticles nucleation surface interactions
- Antimicrobial Peptides and Activities
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Insect and Pesticide Research
- Fermentation and Sensory Analysis
- Advanced Fluorescence Microscopy Techniques
- Indoor Air Quality and Microbial Exposure
- Insect and Arachnid Ecology and Behavior
BOKU University
2019-2024
TU Wien
2011-2021
Universität Innsbruck
2017-2021
Swedish University of Agricultural Sciences
2018
Abstract Background Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevance when the prey plant pathogen, providing strategy for biological control of pests protection. Probably, most studied biocontrol agents are species genus Hypocrea / Trichoderma . Results Here we report an analysis genome sequences two atroviride (teleomorph atroviridis ) and virens (formerly Gliocladium , teleomorph ), comparison with reesei jecorina ). These three display...
Abstract Background Trichoderma is a genus of mycotrophic filamentous fungi (teleomorph Hypocrea ) which possess bright variety biotrophic and saprotrophic lifestyles. The ability to parasitize and/or kill other (mycoparasitism) used in plant protection against soil-borne fungal diseases (biological control, or biocontrol). To investigate mechanisms mycoparasitism, we compared the transcriptional responses cosmopolitan opportunistic species powerful biocontrol agents atroviride T. virens...
Unlike most other fungi, molds of the genus Trichoderma (Hypocreales, Ascomycota) are aggressive parasites fungi and efficient decomposers plant biomass. Although nutritional shifts common among hypocrealean there no examples such broad substrate versatility as that observed in Trichoderma. A phylogenomic analysis 23 (including nine spp. related Escovopsis weberi) revealed has evolved from an ancestor with limited cellulolytic capability fed on either or arthropods. The evolutionary genes...
Abstract Background The identification and characterization of the transcriptional regulatory networks governing physiology adaptation microbial cells is a key step in understanding their behaviour. One such wide-domain circuit, essential to all cells, carbon catabolite repression (CCR): it allows cell prefer some sources, whose assimilation high nutritional value, over less profitable ones. In lower multicellular fungi, C2H2 zinc finger CreA/CRE1 protein has been shown act as repressor this...
ABSTRACT The scarcity of fresh water in the Mediterranean region necessitates search for halotolerant agents biological control plant diseases that can be applied arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Psammocinia sp. sponges, greatest number isolates belong to longibrachiatum - Hypocrea orientalis species pair (9), H. atroviridis/T. atroviride and T. harzianum complex (7), all which are known high mycoparasitic potential. In...
Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression obligatorily dependent on function protein methyltransferase LAE1. The Aspergillus nidulans orthologue LAE1 - LaeA part VELVET complex consisting LaeA, VeA VelB that regulates secondary metabolism sexual as well asexual reproduction. Here we have therefore investigated VEL1, T. A. VeA. Deletion vel1 locus causes a complete light-independent loss conidiation, impairs...
Abstract The roots of Arabidopsis thaliana host diverse fungal communities that affect plant health and disease states. Here, we sequence the genomes 41 isolates representative A. root mycobiota for comparative analysis with other 79 plant-associated fungi. Our analyses indicate members evolved from ancestors lifestyles retain large repertoires cell wall-degrading enzymes (PCWDEs) effector-like small secreted proteins. We identify a set 84 gene families associated endophytism, including...
Due to low iron availability under environmental conditions, many microorganisms excrete iron-chelating agents (siderophores) cover their demands. A novel screening approach for the detection of siderophores using liquid chromatography coupled high-resolution tandem mass spectrometry was developed study production extracellular 10 wild-type Trichoderma strains. For annotation siderophores, an in-house library comprising 422 known microbial established. After 96 h cultivation, 18 different...
Significance Many organisms are specialists living within a narrow range of conditions. Pathogens often adapted to efficiently exploit only few hosts species, or sometimes, some genotypes species. The genomes such parasites predicted maintain genes critical for host utilization and lose no longer necessary outside their constrained lifestyle. We demonstrate that the genomic content fungal pathogen specialized attack consume fungus cultivated by ants meets these predictions. Despite reduced...
ABSTRACT Species of the fungal genus Trichoderma (Hypocreales, Ascomycota) are well-known for their production various secondary metabolites. Nonribosomal peptides and polyketides represent a major portion these products. In recent phylogenomic investigation polyketide synthase (PKS)-encoding genes, pks4 from T. reesei was shown to be an orthologue pigment-forming PKSs involved in synthesis aurofusarin bikaverin Fusarium spp. this study, we show that deletion gene results loss green conidial...
Background Trichoderma reesei, a mitosporic green mould, was recognized during the WW II based on single isolate from Solomon Islands and since then used in industry for production of cellulases. It is believed to be an anamorph (asexual stage) common pantropical ascomycete Hypocrea jecorina. Methodology/Principal Findings We combined molecular evolutionary analysis multiple methods phenotype profiling order reveal genetic relationship T. reesei H. The resulting data show that isolates which...
One of the biggest threats to coffee growers in East Africa are emerging vascular wilt diseases (tracheomycosis) caused by Fusarium spp. Many Trichoderma species known be natural antagonists these pathogens and widely used biological control fungal plant diseases. More recently, several spp., which exhibited high antifungal activity have been isolated as endophytes. Consequently, we investigated presence antagonistic endophytic from roots healthy plants (Coffea arabica) major growing regions...
Trichoderma guizhouense NJAU 4742 (Harzianum clade) can suppress the causative agent of banana wild disease Fusarium oxysporum f. sp. cubense 4 (Foc4). To identify genes involved in this trait, we used T-DNA insertional mutagenesis and isolated one mutant that was unable to overgrow Foc4 had reduced antifungal ability. Using high-efficiency thermal asymmetric interlaced-PCR, located terminator a neutral metalloprotease gene (encoding MEROPS family M35 protease), which named nmp1. The...
Fourteen Trichoderma (Hypocreales) species were identified during a survey of the genus in South Africa. These include T. afroharzianum, asperelloides, asperellum, atrobrunneum, atroviride, camerunense, gamsii, hamatum, koningii, koningiopsis, saturnisporum, spirale, virens, and viride. Ten these not known to occur Africa prior this investigation. Five additional novel are described here as beinartii, caeruleimontis, chetii, restrictum, undulatum. display morphological traits that typical...
We have previously reported that the prominent industrial enzyme producer Trichoderma reesei (teleomorph Hypocrea jecorina; Hypocreales, Ascomycota, Dikarya) has a genetically isolated, sympatric sister species devoid of sexual reproduction and which is constituted by majority anamorphic strains attributed to H. jecorina/T. reesei. In this paper we present formal taxonomic description new species, T. parareesei, complemented multivariate phenotype profiling molecular evolutionary...
Pectin is one of the major and most complex plant cell wall components that needs to be overcome by microorganisms as part their strategies for invasion or nutrition. Microbial pectinolytic enzymes therefore play a significant role plant-associated decomposition recycling organic matter. Recently, comparative studies revealed gene copy number expansion polysaccharide lyase 1 (PL1) pectin/pectate family in Clonostachys rosea genome, while only low numbers were found Trichoderma species. Both...
ABSTRACT Trichoderma species are abundant in different agricultural habitats, but some representatives of this genus, mainly clade Longibrachiatum members also emerging as causative agents various human diseases with even fatal outcome. Strains these frequently show resistance to commonly used azole antifungals. Based on previous data it is hypothesized that isolates identified infections derive from environmental—including agricultural—origins. We examined longibrachiatum Rifai and...
In the course of investigations on peptaibol chemodiversity from marine-derived Trichoderma spp., five new 15-residue peptaibols named pentadecaibins I-V (1-5) were isolated solid culture strain sp. MMS1255 belonging to T. harzianum species complex. Phylogenetic analyses allowed precise positioning close lentiforme lineage inside Harzianum clade. Peptaibol sequences elucidated basis their MS/MS fragmentation and extensive 2D NMR experiments. Amino acid configurations determined by Marfey's...
The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well the rhizosphere Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests Colombian Amazonia. DNA barcoding 107 strains based on internal transcribed spacers 1 2 (ITS1 2) ribosomal RNA gene cluster partial sequence translation elongation factor alpha (tef1) revealed that dominated (71 %) by three common cosmopolitan species, namely harzianum sensu lato (41 %), spirale (17...
Mycoparasites, e.g. fungi feeding on other fungi, are prominent within the genus Trichoderma and represent a promising alternative to chemical fungicides for plant disease control. We previously showed that seven-transmembrane receptor Gpr1 regulates mycelial growth asexual development governs mycoparasitism-related processes in atroviride. now describe identification of genes being targeted by under mycoparasitic conditions. The identified gene set includes candidate, sfp2, encoding protein...