A5055870899- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Essential Oils and Antimicrobial Activity
- Animal Virus Infections Studies
- Respiratory viral infections research
- Phytochemistry and Biological Activities
- Herpesvirus Infections and Treatments
- Psidium guajava Extracts and Applications
- Advanced Photocatalysis Techniques
- Phytochemistry and Bioactivity Studies
- Antimicrobial Peptides and Activities
- Parvovirus B19 Infection Studies
- Hepatitis B Virus Studies
- Plant Virus Research Studies
- TiO2 Photocatalysis and Solar Cells
- Synthesis and bioactivity of alkaloids
- Pharmacological Effects of Natural Compounds
- Virology and Viral Diseases
- Toxin Mechanisms and Immunotoxins
- Chemical synthesis and alkaloids
- Nuts composition and effects
- Hepatitis C virus research
- Botanical Research and Applications
- Liver Disease Diagnosis and Treatment
- Garlic and Onion Studies
Tunis El Manar University
2017-2025
Center of Biotechnogy of Borj Cédria
2014-2024
University of Monastir
2006-2014
ABSTRACT The sequencing of the VP1 hypervariable region human enterovirus (HEV) genome has become reference test for typing field isolates. This study describes a new strategy HEV at serotype level that uses reverse transcription-PCR assay targeting central part VP2 capsid protein. Two pairs primers were used to amplify fragment 584 bp (with PV-1 sequence) or it (368 bp) typing. For few strains not amplified by first PCR, seminested enhanced sensitivity (which was found be approximately 10...
The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) Herpes simplex virus type 2 (HSV-2) antiviral Chromobacterium violaceum (ATCC 12472 CV026) Pseudomonas aeruginosa PAO1 as starter strains anti-quorum sensing tests. Isoprenoids are main class compounds identified,...
The aim of this study was to investigate the phytochemical composition dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report antimicrobial activities against a wide range bacteria, yeast, fungi. antioxidant were tested 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, 2-2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. also for first time effect swarming motility in Pseudomonas aeruginosa PAO1. Our results showed that two...
Abstract Brown seaweeds are known for their bioactive compounds, particularly sulfated polysaccharides such as fucoidans, which have demonstrated antiviral properties. However, limited studies focused on the potential of fucoidans extracted from Mediterranean brown seaweeds. In this study, two Padina pavonica and Dictyopteris membranacea (Fuc-Pad Fuc-Dic) were collected monastir coasts, Tunisia, a specific extraction protocol was employed to obtain fucoidans. The main objective study...
In this report, Castanea sativa shell extracts have been investigated for their activity against Herpes Virus type 2 (HSV-2). Among four organic only 75% ethanol extract demonstrated HSV-2 with a 50% inhibitory concentration of 6.45 μg/mL. The study the mechanism underlying antiviral strongly inhibits by direct contact and moderately protects cell from virus recognition binding. active compound has identified as oleuropein HPLC-DAD-ESI-MSn. This which is widely known in Oleaceae family...
The present study was the first to evaluate phytochemical composition, antioxidant, antimicrobial, antibiofilm, and anti-quorum sensing potential of Allium subhirsutum L. (hairy garlic) aqueous extract through in vitro silico studies. profile revealed presence saponins, terpenes, flavonols/flavonones, flavonoids, fatty acids, particularly with flavonoids (231 ± 0.022 mg QE/g extract), tannins (159 0.006 TAE/g phenols (4 0.004 GAE/g extract). Gas chromatography–mass spectrometry (GC–MS)...
Recombination between two strains is a known phenomenon for enteroviruses replicating within single cell. We describe recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 VP2 coding regions, respectively. The was neutralized by polyclonal CV-B3-specific antiserum but not CV-B4-specific antiserum. nucleotide sequence analysis whole structural genomic region showed occurrence recombination event at position 1950 VP3...
Asphodelus tenuifolius Cav. (A. tenuifolius) is a medicinal plant with long history of traditional use to treat ailments. In this study, total phenolic and flavonoid content evaluation using LC-ESI/MS analysis various biological activities (antioxidant, antibacterial, antifungal, antiviral cytotoxicity) organic extracts from the aerial parts A. were analyzed. ADME tools used predict potential identified compounds most potent extract as specific drugs. As shown, results chloroformic allowed...
Abstract Commiphora gileadensis, locally known as becham, is a plant used in traditional Arabian medicine for treating headache, constipation, stomach, joint pain, and inflammatory disorders. Several studies have reported its antibacterial properties; however, no study has demonstrated antiviral activity. This aimed to evaluate the activity of C. gileadensis well isolate active compound investigate mode action. was evaluated using 4 viruses, herpes simplex virus type 2 (HSV-2), respiratory...
Abstract Aims This study aimed to determine the antibacterial and antileishmanial potential of Micromeria nervosa extracts. The identification compound its molecular mechanism action have also been undertaken. Methods results Ethanol extract showed high polyphenol content diethyl ether exhibited DPPH scavenging low beta-carotene bleaching activity (IC50 = 13.04 ± 0.99 200.18 3.32 μg mL−1, respectively). However, displayed against Gram-positive strains including methicillin-resistant...
Decay Accelerating Factor (DAF) and Coxsackievirus-Adenovirus Receptor (CAR) have been identified as cellular receptors for Coxsackie B viruses (CV-B). The aim of this study is to elucidate the different binding properties CV-B serotypes find out if there are any amino acid changes that could be associated phenotypes.Twenty clinical isolates were tested on CaCo-2 cell line using anti-DAF (BRIC216) anti-CAR (RmcB) antibodies. CV-B3 Nancy prototype strain a recombinant (Rec, CV-B3/B4) in...
To investigate the incidence, viral load and genetic diversity of bovine rotaviruses strains in Tunisia.A total 169 faecal specimens, collected from diarrhoeic calves several farms located central eastern regions Tunisia, between January 2006 October 2010, were analysed by semi-nested multiplex RT-PCRs for P G genotypes identification or genotyped DNA sequencing. Positive samples tested TaqMan real-time RT-PCR to quantify load. Group A detected 15·4% (26/169) studied cases diarrhoea....