In 1957, Tunisia introduced 117 species of Eucalyptus; they have been used as fire wood, for the production mine wood and to fight erosion. Actually, Eucalyptus essential oil is traditionally treat respiratory tract disorders such pharyngitis, bronchitis, sinusitis. A few investigations were reported on biological activities oils worldwide. Tunisia, our previous works conducted in 2010 2011 had first reports study antibacterial against reference strains. At that time it was not possible...

This prospective study, conducted from January 2003 to June 2005, investigated the incidence and clinical role of various enteric viruses responsible for infantile gastroenteritis in 632 Tunisian children presenting dispensaries (380 children) or hospitalized (252 acute diarrhea. At least one virus was found each 276 samples (43.7%). A single pathogen observed 234 samples, mixed infections were 42 samples. In terms frequency, rotavirus norovirus detected 22.5 17.4% respectively, followed by...

We have investigated the antibacterial, antifungal and cytotoxic activities of two flavonoids isolated from Retama raetam flowers using disc diffusion micro-dilution broth methods. The activity was tested against Hep-2 cells MTT assay. compounds licoflavone C (1) derrone (2) were active Pseudomonas aeruginosa Escherichia coli (7.81-15.62 μg/mL) showed important activity. Strong Candida species (7.81 for example found with compound 2. also strong cytotoxicity cells. These may be interesting...

The essential oils of fifteen Eucalyptus species harvested from the Jbel Abderrahman and Korbous arboreta (North East Tunisia) were screened for their antibacterial activities by agar disc diffusion method. Eighteen major components as identified GC/FID GC/MS selected a study chemical biological activity variability. main one was 1,8-cineole, followed spathulenol, trans-pinocarveol, α-pinene, p-cymene, globulol, cryptone, β-phellandrene, viridiflorol, borneol, limonene isospathulenol....

The latex of fig fruit (Ficus carica) is used in traditional medicine for the treatment skin infections such as warts and also diseases possible viral origin. Five extracts (methanolic, hexanic, ethyl acetate, hexane-ethyl acetate (v/v) chloroformic) this species were investigated vitro their antiviral potential activity against herpes simplex type 1 (HSV-1), echovirus 11 (ECV-11) adenovirus (ADV). To evaluate capacity to inhibit replication viruses, following assays performed: adsorption...

Abstract The in vitro antiviral activity of dermaseptins (S1–S5) against herpes simplex virus type 1 (HSV1) was investigated. These peptides were incubated with the and its target cells under various conditions, their effects examined by cytopathic effect inhibition assay or reduction yield Hep‐2 cell cultures as well direct immunofluorescence. Dermaseptin S4 displayed strongest HSV1, at micromolar doses. Experiments including acyclovir a reference agent performed to investigate mode action...

Fifteen species of Tunisian traditional medicinal plants, belonging to 10 families, were selected for this study. They Inula viscosa (L.) Ait and Reichardia tingitana Roth ssp. discolor (Pom.) Batt. (Asteraceae), Mesembryanthemum cristallinum L. M. nodiflorum (Aizoaceae), Arthrocnemum indicum (Willd.) Moq., Atriplex inflata Muell., A. parvifolia Lowe var. ifiniensis (Caball) Maire, Salicornia fruticosa (Chenopodiaceae), Cistus monspeliensis (Cistaceae), Juniperus phoenicea (Cupressaceae),...

The aim of the present study was investigation antimicrobial and antioxidant activities aqueous methanolic extracts obtained from some Tunisian vegetables. activity evaluated by microdilution method. Total phenolic contents were determined Folin–Ciocalteu colorimetric using DPPH assay. Among tested extracts, extract Apium graveolens had best antifungal against Candida albicans, kreussei parapsilosis with MIC values ranging between 0.08 0.31 mg/ml. Concerning we conclude that A. graveolens,...

The chemical composition, antioxidant, cytotoxic and antiviral activities of the essential oil obtained by hydrodistillation from aerial parts Teucrium pseudochamaepitys (Lamiaceae) collected Zaghouan province Tunisia are reported. was analyzed gas chromatography equipped with a flame ionization detector (GC-FID) coupled mass spectrometry (GC/MS). Thirty-one compounds were identified representing 88.6% total oil. Hexadecanoic acid found to be most abundant component (26.1%) followed...

Coxsackieviruses B (CV‑B) are known as the most common viral cause of human heart infections. The aim present study was to assess potential role CV‑B in etiology infectious disease hospitalized patients. is based on blood, pericardial fluid and biopsies from 102 patients 100 control subjects. All samples were examined for detection specific enteroviral genome using reverse transcription polymerase chain reaction (RT‑PCR) sequence analysis. Immunohistochemical investigations capsid protein,...

Olea europaea L. is one of the most important fruit trees in Tunisia because its content many potentially bioactive compounds. The aim this study was to evaluate chemical composition, antibiofilm, antiradical and acethylcholinesterase inhibitory activities from four Tunisian cultivars L., i.e. 'Chetoui', 'Meski', 'Oueslati' 'Jarboui'. By means standardized methods, total phenols were determined some them characterized by HPLC. flavonoids contents found be highest leaves Chetoui cultivar....

The antimicrobial properties of essential oil from various Santolina species have not been investigated enough in the previous studies dealing with biological activities medicinal plants. In Tunisia, chamaecyparissus L. (Asteraceae) is only recorded and used as vermifuge emmenagogue. chemical composition, antibacterial antifungal oils flowerheads roots spontaneous S. growing Tunisia composition which leads to Tunisian chemotype are here for first time. Essential isolated by hydro...

ABSTRACT The sequencing of the VP1 hypervariable region human enterovirus (HEV) genome has become reference test for typing field isolates. This study describes a new strategy HEV at serotype level that uses reverse transcription-PCR assay targeting central part VP2 capsid protein. Two pairs primers were used to amplify fragment 584 bp (with PV-1 sequence) or it (368 bp) typing. For few strains not amplified by first PCR, seminested enhanced sensitivity (which was found be approximately 10...

Human noroviruses (NoVs) cause epidemic and endemic acute gastroenteritis in children adults. To study the prevalence genetic diversity of NoV Tunisia, a total 788 fecal samples were collected during 4-year period region Monastir, from 12 years age or younger, hospitalized presenting dispensaries with symptoms gastroenteritis. was detected by reverse transcription-PCR confirmed sequence analysis. This is first report that describes molecular epidemiology Tunisian children: NoVs characterized...

Aims: A prospective study was performed to characterize the main human enteric viruses able persist in sewage samples and shellfish tissues, establish correlation between environmental strains viral infantile diarrhoea observed same area during period. Methods Results: total of 250 (raw treated) 60 were collected January 2003 April 2007 Monastir region, Tunisia. Group rotavirus (RVA) detected 80 (32%) samples, norovirus (NoV) 11 (4·4%) adenovirus (AdV) 1 (0·4%). Among near effluents, one...