A5062079123- Computational Physics and Python Applications
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Advanced Electron Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Hydrocarbon exploration and reservoir analysis
- Geomagnetism and Paleomagnetism Studies
- Immune Cell Function and Interaction
- Plant-Microbe Interactions and Immunity
- Reproductive System and Pregnancy
- Biofuel production and bioconversion
- Blood groups and transfusion
- Bacteriophages and microbial interactions
- Systemic Lupus Erythematosus Research
- Protein Degradation and Inhibitors
- Fungal and yeast genetics research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
Leiden University Medical Center
2019-2024
Institute of Science and Technology Austria
2024
Universität Innsbruck
2018
Abstract IgG3 is unique among the IgG subclasses due to its extended hinge, allotypic diversity and enhanced effector functions, including highly efficient pathogen neutralisation complement activation. It also underrepresented as an immunotherapeutic candidate, partly a lack of structural information. Here, we use cryoEM solve structures antigen-bound alone in complex with components. These reveal propensity for IgG3-Fab clustering, which possible IgG3-specific flexible upper hinge region...
The classical complement pathway is activated by antigen-bound IgG antibodies. Monomeric must oligomerize to activate via the hexameric C1q complex, and hexamerizing mutants of appear as promising therapeutic candidates. However, structural data have shown that it not necessary bind all six arms initiate complement, revealing a symmetry mismatch between C1 complex has been adequately explained. Here, we use DNA nanotechnology produce specific nanostructures template antigens thereby...
Abstract Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and thus critically dependent the availability open-source software for accurate well high-throughput tomogram segmentation. There need more user-friendly, flexible comprehensive that offers an insightful overview all steps involved preparing automated segmentations. Here, we present Ais:...
Mycoparasites, e.g. fungi feeding on other fungi, are prominent within the genus Trichoderma and represent a promising alternative to chemical fungicides for plant disease control. We previously showed that seven-transmembrane receptor Gpr1 regulates mycelial growth asexual development governs mycoparasitism-related processes in atroviride. now describe identification of genes being targeted by under mycoparasitic conditions. The identified gene set includes candidate, sfp2, encoding protein...
Autoantibodies directed against complement component C1q are commonly associated with autoimmune diseases, especially systemic lupus erythematosus. Importantly, these anti-C1q autoantibodies specific for ligand-bound, solid-phase and do not bind to fluid-phase C1q. In patients anti-C1q, levels in the normal range, thus depleting. To study human at molecular level, we isolated C1q-reactive B cells recombinantly produced nine monoclonal antibodies (mAbs) from four different healthy...
DNA origami nanostructures generally require a single scaffold strand of specific length, combined with many small staple strands. Ideally, the length should be dictated by size designed nanostructure. However, synthesizing arbitrary-length single-stranded in sufficient quantities is difficult. Here, we describe straightforward and accessible method to produce defined-length ssDNA scaffolds using PCR subsequent selective enzymatic digestion T7 exonuclease. This approach produced higher...
Carbamylation is a post-translational modification that can be detected on range of proteins, including immunoglobulin (Ig)G, in several clinical conditions. Carbamylated IgG (ca-IgG) was reported to lose its capacity trigger complement activation, but the mechanism remains unclear. Because C1q binds with high affinity hexameric IgG, we analyzed whether carbamylation affects binding C1q, hexamerization and complement-dependent cytotoxicity (CDC). Synovial tissues rheumatoid arthritis (RA)...
Complement activation protects against infection but also contributes to pathological mechanisms in a range of clinical conditions such as autoimmune diseases and transplant rejection. Complement-inhibitory drugs, either approved or development, usually act systemically, thereby increasing the risk for infections. We therefore envisioned novel class bispecific antibodies (bsAbs) which are capable site-directed complement inhibition by bringing endogenous regulators vicinity defined cell...
Structure-inspired peptide design and RaPID selection identifies a macrocyclic with immunotherapeutic potential, inspires development of complement-modulating drugs.
Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and thus critically dependent the availability open-source software for accurate well high-throughput tomogram segmentation. There need more user-friendly, flexible comprehensive that offers an insightful overview all steps involved preparing automated segmentations. Here, we present Ais: dedicated...
Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and thus critically dependent the availability open-source software for accurate well high-throughput tomogram segmentation. There need more user-friendly, flexible comprehensive that offers an insightful overview all steps involved preparing automated segmentations. Here, we present Ais: dedicated...
Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and thus critically dependent the availability open-source software for accurate well high-throughput tomogram segmentation. There need more user-friendly, flexible, comprehensive that offers an insightful overview all steps involved preparing automated segmentations. Here, we present Ais: dedicated...
Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and thus critically dependent the availability open-source software for accurate well high-throughput tomogram segmentation. There need more user-friendly, flexible, comprehensive that offers an insightful overview all steps involved preparing automated segmentations. Here, we present Ais: dedicated...
Abstract The classical complement pathway is activated by antigen-bound IgG antibodies. Monomeric must oligomerize to activate via the hexameric C1q complex, and hexamerizing mutants of appear as promising therapeutic candidates. However, structural data have shown that it not necessary bind all six arms initiate complement, revealing a symmetry mismatch between C1 which has been adequately explained. Here we use DNA nanotechnology produce specific nanostructures template antigens, thereby...