A5075430333- Psychedelics and Drug Studies
- Silymarin and Mushroom Poisoning
- Fungal Biology and Applications
- Chemical synthesis and alkaloids
- Cholinesterase and Neurodegenerative Diseases
- Microbial Natural Products and Biosynthesis
- Phenothiazines and Benzothiazines Synthesis and Activities
- Polyamine Metabolism and Applications
- Ion channel regulation and function
- Gut microbiota and health
- Chemical Synthesis and Analysis
Friedrich Schiller University Jena
2022-2024
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2022-2024
Abstract Psilocybin, the natural hallucinogen produced by Psilocybe (“magic”) mushrooms, holds great promise for treatment of depression and several other mental health conditions. The final step in psilocybin biosynthetic pathway, dimethylation tryptophan-derived intermediate norbaeocystin, is catalysed PsiM. Here we present atomic resolution (0.9 Å) crystal structures PsiM trapped at various stages its reaction cycle, providing detailed insight into SAM-dependent methylation mechanism....
Abstract Psilocybe magic mushrooms are best known for their main natural product, psilocybin, and its dephosphorylated congener, the psychedelic metabolite psilocin. Beyond tryptamines, secondary metabolome of these fungi is poorly understood. The genomes five species ( P. azurescens , cubensis cyanescens mexicana serbica ) were browsed to understand more profoundly common species‐specific metabolic capacities. genomic analyses revealed a much greater yet unexplored diversity than evident...
Abstract l ‐(+)‐Muscarine ( 1 )‐producing mushrooms pose a severe threat to human health as ingestion can result in circulatory collapse or even death. However, their metabolic profile is surprisingly poorly understood, including knowledge of poison release and potentially toxic congeners. In the mycelium ‐producing fool's funnel mushroom Clitocybe rivulosa , we identified 4’‐phosphomuscarine 2 ) major natural product. Its structure was elucidated by high‐resolution mass spectrometry,...
Psilocybin, the natural hallucinogen from Psilocybe (magic) mushrooms, is a highly promising drug candidate for treatment of depression and several other mental health conditions. Biosynthesis psilocybin amino acid l‐ tryptophan involves four strictly sequential modifications. The third these, ATP‐dependent phosphorylation intermediate 4‐hydroxytryptamine, catalysed by PsiK. Here we present crystallographic analysis structure‐based mutagenesis study this kinase, providing insight into its...
Abstract The Psilocybe cubensis SAM‐dependent methyltransferase, PsiM, catalyzes the last step in biosynthesis of psilocybin. Likely evolved from monomethylating RNA methyltransferases, PsiM acquired a key amino acid exchange secondary sphere active site, M247 N, which is responsible for its capacity to dimethylate. Two variants, N247M and N247A , were generated further examine role Asn247 mono‐ dimethylation PsiM. Herein, we present kinetic profiles both variants crystal structures at...
l‐(+)‐Muscarine (1)‐producing mushrooms pose a severe threat to human health as ingestion can result in circulatory collapse or even death. However, their metabolic profile is surprisingly poorly understood, including knowledge of poison release and potentially toxic congeners. In the mycelium 1‐producing fool’s funnel mushroom Clitocybe rivulosa, we identified 4’‐phosphomuscarine (2) major natural product. Its structure was elucidated by high‐resolution mass spectrometry, nuclear magnetic...