A5103275425- Histone Deacetylase Inhibitors Research
- Mycorrhizal Fungi and Plant Interactions
- Epigenetics and DNA Methylation
- Fungal Biology and Applications
- Ubiquitin and proteasome pathways
- Digital Marketing and Social Media
- Technology Adoption and User Behaviour
- Glycosylation and Glycoproteins Research
- Plant Molecular Biology Research
- Botany and Plant Ecology Studies
- Plant Pathogens and Fungal Diseases
- Coleoptera Taxonomy and Distribution
- Cancer-related gene regulation
- Coleoptera: Cerambycidae studies
- Protein Degradation and Inhibitors
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Library Collection Development and Digital Resources
- Cancer-related Molecular Pathways
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Yeasts and Rust Fungi Studies
- Genomics and Chromatin Dynamics
- Nematode management and characterization studies
- Plant nutrient uptake and metabolism
Friedrich Schiller University Jena
2008-2024
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2024
University of California, San Diego
2021-2024
University of Würzburg
2016-2024
University of Freiburg
2012-2021
Howard Hughes Medical Institute
2021
GAF AG
2017
Northwestern University
2014
Ludwig-Maximilians-Universität München
2012-2013
Helmholtz Zentrum München
2008-2011
The classification of Phellinus s.l., Inonotus s.l. and the phylogenetic relationships allied genera were studied using nuc-lsu rDNA sequence data. worldwide taxon sampling comprised 107 species, 99 them belonging to Hymenochaetales. trees discussed in relation morphological anatomical features fruit bodies. Hymenochaetales formed no monophyletic group several non-Hymenochaetales appeared as intermingled with Trichaptum abietinum Oxyporus populinus showed certain affinities within...
The classification of Phellinus s.l., Inonotus s.l. and the phylogenetic relationships allied genera were studied using nuc-lsu rDNA sequence data. worldwide taxon sampling comprised 107 species, 99 them belonging to Hymenochaetales. trees discussed in relation morphological anatomical features fruit bodies. Hymenochaetales formed no monophyletic group several non-Hymenochaetales appeared as intermingled with Trichaptum abietinum Oxyporus populinus showed certain affinities within...
Histone deacetylase 2 (HDAC2) is relevant for homeostasis and plays a critical role in gastrointestinal cancers. Here, we report that post-translational modification of endogenous HDAC2 with small ubiquitin-related modifier 1 (SUMO1) new regulatory switch the tumor suppressor p53. Sumoylation at lysine 462 allows binding to Moreover, sumoylated previously not recognized biologically site-specific Deacetylation p53 320 by blocks recruitment into promoter-associated complexes p53-dependent...
Potassium channels allow for the passive movement of potassium ions across cell membrane and are instrumental in controlling potential all types. Quaternary ammonium (QA) compounds block have long been used to study functional structural properties these channels. Here we describe interaction between three symmetrical hydrophobic QAs prokaryotic channel KcsA. The structures demonstrate presence a pocket inner helices KcsA provide insight into binding site blocking mechanism QAs. also reveal...
The signal transducer and activator of transcription 5 (STAT5) regulates differentiation, survival, proliferation transformation hematopoietic cells. Upon cytokine stimulation, STAT5 tyrosine phosphorylation (pYSTAT5) is transient, while in diverse neoplastic cells persistent overexpression enhanced pYSTAT5 are frequently found. Post-translational modifications might contribute to activation the context transformation, but strength duration incompletely understood. We found that...
Epigenetic modifications of histone tails play an essential role in the regulation eukaryotic transcription. Writer and eraser enzymes establish maintain epigenetic code by creating or removing posttranslational marks. Specific binding proteins, called readers, recognize mediate signalling. Here, we present a versatile assay platform for investigation interaction between methyl lysine readers their ligands. This can be utilized screening small-molecule inhibitors such protein–protein...
Plants tightly control growth of their lateral organs, which led to the concept apical dominance. However, outgrowth dormant primordia is sensitive plant's nutritional status, resulting in an immense plasticity plant architecture. While impact hormonal regulation on dominance well characterized, prime importance sugar signaling unleash organ formation has just recently emerged. Here, we aimed identify transcriptional regulators, trade-off between versus organs. Making use locally inducible...
We used specific antibodies recognizing the receptor 1 (IFNAR1) and recently cloned 2.2 (IFNAR2.2) chains of human type I interferon complex to demonstrate that β (IFN-β)-specific receptor-associated phosphoprotein is IFNAR2.2 not an unknown or additional component. Immunoprecipitation experiments demonstrated present in Daudi cells as a cell surface protein approximately 90-100 kDa, which tyrosine-phosphorylated associated with IFNAR1, upon stimulation IFN-β. was detected IFNAR1 stimulated...
The transcription factor nuclear factor-κB (NF-κB) is crucial for the maintenance of homeostasis. It incompletely understood how NF-κB and crosstalk with other factors are controlled. Here, we demonstrate that epigenetic regulator histone deacetylase 2 (HDAC2) activates in transformed primary cells. This function depends on both, catalytic activity an intact HDAC2 sumoylation motif. Several mechanisms account induction through HDAC2. expression wild-type can increase presence NF-κB. In...
The methyllysine reader protein Spindlin1 has been implicated in the tumorigenesis of several types cancer and may be an attractive novel therapeutic target. Small-molecule inhibitors should valuable as chemical probes well potential new therapeutics. We applied iterative virtual screening campaign, encompassing structure- ligand-based approaches, to identify from databases commercially available compounds. Our silico studies coupled with vitro testing were successful identifying inhibitors....
Late‐stage colorectal cancer (CRC) is still a clinically challenging problem. The activity of the tumor suppressor p53 regulated via post‐translational modifications (PTMs). While relevance C‐terminal acetylation for transcriptional regulation well defined, it unknown whether this PTM controls mitochondrially mediated apoptosis directly. We used wild‐type or p53‐negative human CRC cells, cells with acetylation‐defective p53, transformation assays, organoids, and xenograft mouse models to...
To determine whether the human antibody (Ab) repertoire to Haemophilus influenzae type b capsular polysaccharide (Hib PS) could be studied at molecular level with phage display technology, we constructed a Fab library by using peripheral blood from vaccinated adult. Phage were selected based on Hib PS binding. Two distinct PS-specific clones identified whose fragments used same V(H) region paired two different V(L) regions. The regions derived independent rearrangements of A2c gene Jkappa1,...
Carotenoids are constituents of the photosynthetic apparatus and essential for plant survival because their involvement in protection chlorophylls against photooxidation. Certain classes herbicides interfering with carotenoid biosynthesis leading to pigment destruction a bleached phenotype. One important target site bleaching is enzyme phytoene desaturase catalysing desaturation ζ-carotene. This enzymatic reaction can be inhibited by norflurazon or fluridone. We have transformed tobacco...