A5105926410- Plant Stress Responses and Tolerance
- Plant and Biological Electrophysiology Studies
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Ion channel regulation and function
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Calcium signaling and nucleotide metabolism
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Transgenic Plants and Applications
- GABA and Rice Research
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Aluminum toxicity and tolerance in plants and animals
- Plant Diversity and Evolution
- Ion Channels and Receptors
- Genomics and Phylogenetic Studies
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
University of Würzburg
2013-2024
Leibniz Association
1998-2023
Vienna Biocenter
2023
Spital Uster
2022
Universität Hamburg
1999-2017
Durham University
2012
University of Potsdam
2004
Leibniz University Hannover
1994-1996
Institut für biologische Forschung
1995
Philipps University of Marburg
1993
Plasma membrane-borne pattern recognition receptors, which recognize microbe-associated molecular patterns and endogenous damage-associated patterns, provide the first line of defense in innate immunity. In plants, leucine-rich repeat receptor kinases fulfill this role, as exemplified by FLS2 EFR, receptors for flagellin elongation factor Tu. Here we examined perception peptide 1 (AtPep1), an Arabidopsis identified earlier shown to be perceived protein kinase PEPR1. Using seedling growth...
Abstract Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, Arabidopsis hairs. Using intracellular microelectrodes show membrane depolarization, response to IAA concentration- pH-dependent manner. This depolarization strongly impaired aux1 mutants, indicating that AUX1 major transporter for uptake Local application triggers Ca...
Here we report on the molecular identification, guard cell expression and functional characterization of At GORK, an Arabidopsis thaliana goutward rectifying K + channel. GORK represents a new member plant Shaker channel superfamily. When heterologously expressed in Xenopus oocytes gene product mediated depolarization‐activated currents. In agreement with delayed outward rectifier intact cells protoplasts thereof, is activated voltage‐ potassium‐dependent manner. Furthermore, single...
Auxin-induced growth of coleoptiles depends on the presence potassium and is suppressed by K + channel blockers. To evaluate role channels in auxin-mediated growth, we isolated functionally expressed ZMK1 ZMK2 ( Zea mays 1 2), two from maize coleoptiles. In experiments, time course auxin-induced expression coincided with kinetics coleoptile elongation. Upon gravistimulation seedlings, followed gravitropic-induced auxin redistribution. increased even before a bending was observed. The...
The perception of microbes by plants involves highly conserved molecular signatures that are absent from the host and collectively referred to as microbe-associated patterns (MAMPs). Arabidopsis pattern recognition receptors FLAGELLIN-SENSING 2 (FLS2) EF-Tu receptor (EFR) represent genetically well studied paradigms mediate defense against bacterial pathogens. Stimulation these through their cognate ligands, flagellin or elongation factor Tu, leads a response ultimately increased resistance....
Species-preferential osmotic pollen tube burst and sperm discharge in maize involve induced opening of the tube-expressed potassium channel KZM1 by egg apparatus-derived defensin-like protein ZmES4.
Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study regulation membrane water fluxes. Two plasma intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs characterized aquaporins Xenopus laevis oocytes. Osmotic permeability (Pf) was 10 times higher SsAQP2-expressing oocytes than SsAQP1-expressing found be glycerol permeable, SsAQP2 inhibited by 0.5 mM HgCl2 1 phloretin. The aquaporin mRNA...
Ion channels in roots allow the plant to gain access nutrients. The composition of individual ion and functional contribution different α-subunits is largely unknown. Focusing on K + -selective channels, we have characterized AtKC1, a new α-subunit from Arabidopsis shaker -like channel family. Promoter-β-glucuronidase (GUS) studies identified AtKC1 expression predominantly root hairs endodermis. Specific antibodies recognized at plasma membrane. To analyze further abundance cells, performed...
Ion channels and solute transporters in the plasma membrane of root hairs are proposed to control nutrient uptake, osmoregulation polar growth. Here we analyzed molecular components potassium transport Arabidopsis by combining K(+)-selective electrodes, reverse transcription-PCR, patch-clamp measurements. The two inward rectifiers AKT1 ATKC1 as well outward rectifier GORK dominated hair K(+) channel pool. Root loss-of-function plants completely lack uptake or exhibited altered properties,...
Calcineurin B-like proteins (CBLs) represent a family of calcium sensor that interact with group serine/threonine kinases designated as CBL-interacting protein (CIPKs). CBL-CIPK complexes are crucially involved in relaying plant responses to many environmental signals and regulating ion fluxes. However, the biochemical characterization has so far been hampered by low activities recombinant CIPKs. Here, we report on an efficient wheat germ extract-based vitro transcription/translation...
Plant growth and development is driven by osmotic processes. Potassium represents the major osmotically active cation in plants cells. The uptake of this inorganic osmolyte from soil Arabidopsis involves a root K+ module consisting two channel α-subunits, AKT1 AtKC1. AKT1-mediated potassium absorption K+-depleted was shown to depend on calcium-sensing proteins CBL1/9 their interacting kinase CIPK23. Here we show that upon activation CBL·CIPK complex low external homomeric channels open at...
The putative two-pore Ca(2+) channel TPC1 has been suggested to be involved in responses abiotic and biotic stresses. We show that AtTPC1 co-localizes with the K(+)-selective AtTPK1 vacuolar membrane. Loss of abolished Ca(2+)-activated slow (SV) currents, which were increased AtTPC1-over-expressing Arabidopsis compared wild-type. A Ca(2+)-insensitive cation channel, as yet uncharacterized, could resolved tpc1-2 knockout plants. kinetics ABA- CO(2)-induced stomatal closure similar wild-type...
Summary The vacuole represents a pivotal plant organelle for management of ion homeostasis, storage proteins and solutes, as well deposition cytotoxic compounds. Ion channels, pumps carriers in the vacuolar membrane under control cytosolic factors provide ionic metabolic homeostasis between this cytoplasm. Here we show that AtTPK1 (KCO1), localized K + channel TPK family, interacts with 14‐3‐3 (general regulating factors, GRFs). Following planta expression TPK1 GRF6 co‐localize at membrane....
Although the concept of botanical carnivory has been known since Darwin's time, molecular mechanisms that allow animal feeding remain unknown, primarily due to a complete lack genomic information. Here, we show transcriptomic landscape Dionaea trap is dramatically shifted toward signal transduction and nutrient transport upon insect feeding, with touch hormone signaling protein secretion prevailing. At same massive induction general defense responses accompanied by repression cell...
Most plants grow and develop by taking up nutrients from the soil while continuously under threat foraging animals. Carnivorous have turned tables capturing consuming nutrient-rich animal prey, enabling them to thrive in nutrient-poor soil. To better understand evolution of botanical carnivory, we compared draft genome Venus flytrap (Dionaea muscipula) with that its aquatic sister, waterwheel plant Aldrovanda vesiculosa, sundew Drosera spatulata. We identified an early whole-genome...
Although there has been long-standing recognition that stimuli-induced cytosolic pH alterations coincide with changes in calcium ion (Ca2+) levels, the interdependence between protons (H+) and Ca2+ remains poorly understood. We addressed this topic using light-gated channelrhodopsin HcKCR2 from pseudofungus Hyphochytrium catenoides, which operates as a H+ conductive, impermeable channel on plasma membrane of plant cells. Light activation Arabidopsis guard cells evokes transient cytoplasmic...