A5025727028- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Bioenergy crop production and management
- Plant Taxonomy and Phylogenetics
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
Leibniz Institute of Plant Genetics and Crop Plant Research
2013-2024
Leibniz Association
2012-2014
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated ordered physical, genetic functional sequence resource that describes barley gene-space in structured whole-genome context. We developed physical map 4.98 Gb, more than 3.90 Gb anchored to high-resolution map. Projecting deep shotgun assembly, complementary DNA RNA data onto this framework supports...
Abstract Inflorescences of the tribe Triticeae, which includes wheat (Triticum sp. L.) and barley (Hordeum vulgare are characterized by sessile spikelets directly borne on main axis, thus forming a branchless spike. ‘Compositum-Barley’ tetraploid ‘Miracle-Wheat’ (T. turgidum convar. compositum (L.f.) Filat.) display noncanonical spike-branching in replaced lateral branch-like structures resembling small-sized secondary spikes. As result this branch formation produces significantly more...
Abstract Viable circadian clocks help organisms to synchronize their development with daily and seasonal changes, thereby providing both evolutionary fitness advantage from an agricultural perspective. A high-resolution mapping approach combined mutant analysis revealed a cereal ortholog of Arabidopsis thaliana LUX ARRHYTHMO/PHYTOCLOCK 1 (LUX/PCL1) as promising candidate for the earliness per se 3 (Eps-3Am) locus in einkorn wheat (Triticum monococcum L.). Using delayed fluorescence...
Abstract Barley (Hordeum vulgare) is an important cereal crop and a model species for Triticeae genomics. To lay the foundation hierarchical map-based sequencing, genome-wide physical map of its large complex 5.1 billion-bp genome was constructed by high-information content fingerprinting almost 600,000 bacterial artificial chromosomes representing 14-fold haploid coverage. The resultant comprises 9,265 contigs with cumulative size 4.9 Gb 96% length barley genome. reliability verified...
Flowering plants with indeterminate inflorescences often produce more floral structures than they require. We found that primordia initiations in barley (Hordeum vulgare L.) are molecularly decoupled from their maturation into grains. While initiation is dominated by flowering-time genes, growth specified light signaling, chloroplast, and vascular developmental programs orchestrated CCT MOTIF FAMILY 4 (HvCMF4), which expressed the inflorescence vasculature. Consequently, mutations HvCMF4...
Abstract Grasses have varying inflorescence shapes; however, little is known about the genetic mechanisms specifying such shapes among tribes. Here, we identify grass-specific TCP transcription factor COMPOSITUM 1 (COM1) expressing in meristematic boundaries of different grasses. COM1 specifies branch-inhibition barley (Triticeae) versus branch-formation non-Triticeae Analyses cell size, walls and transcripts reveal regulates growth, thereby affecting wall properties signaling specifically...
Inflorescence architecture and crop productivity are often tightly coupled in our major cereal crops. However, the underlying genetic mechanisms controlling inflorescence development remain poorly understood. Here, we identified recessive alleles of barley (Hordeum vulgare L.) HvALOG1 (Arabidopsis thaliana LSH1 Oryza G1) that produce non-canonical extra spikelets fused glumes abaxially to central spikelet from upper-mid portion until tip inflorescence. Notably, found exhibits a...
Bread wheat (Triticum aestivum L.) is the most important staple food crop for 35% of world's population. International efforts are underway to facilitate an increase in production, which Wheat Genome Sequencing Consortium (IWGSC) plays role. As part this effort, we have developed a sequence-based physical map chromosome 6A using whole-genome profiling (WGP™). The bacterial artificial (BAC) contig assembly tools fingerprinted (fpc) and linear topological (ltc) were used their assemblies...
Over the last years reference genome sequences of several economically and scientifically important cereals model plants became available. Despite agricultural significance these crops only a small number tools exist that allow users to inspect visualize genomic position genes interest in an interactive manner. We present chromoWIZ, web tool allows visualizing positions relevant comparing data between different plant genomes. Genes can be queried using gene identifiers, functional...
The speciation allele at Eml-A1 of hexaploid wheat, which causes embryo lethality in wheat-rye hybrids, was investigated using cytologically modified genetic stocks. It demonstrated that an extra dose this had no effect on development these hybrids. There positive and, therefore, overcoming the postzygotic barrier. An abortion hybrid embryos earlier stage also not observed. Physical mapping performed chromosome 6A deletion lines. This study revealed location most distal part long arm 6A. To...
Fluorescence in situ hybridization (FISH) has been an efficient way for integrating physical and genetic maps of various small genomes like rice, sorghum <i>Brachypodium</i>; whereas the large barley, repetitive nature genome complicates generation detection single-copy FISH probes. Here, we used exemplarily map contigs a defined interval long arm barley chromosome 2H to evaluate potential FISH-based mapping as supportive means anchoring resolve linear order along respective...
Abstract Grasses have varying inflorescence shapes; however, little is known about the genetic mechanisms specifying such shapes among tribes. We identified grass-specific TCP transcription factor COMPOSITUM 1 (COM1) expressed in meristematic boundaries of different grasses. COM1 specifies branch-inhibition Triticeae (barley) versus branch-formation non-Triticeae Analyses cell size, walls and transcripts revealed barley regulates growth, affecting wall properties signaling specifically to...