A5100412804- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- interferon and immune responses
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Ubiquitin and proteasome pathways
- Herpesvirus Infections and Treatments
- Plant Disease Resistance and Genetics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Hepatitis C virus research
- NF-κB Signaling Pathways
- RNA Research and Splicing
- RNA Interference and Gene Delivery
Czech Academy of Sciences, Institute of Microbiology
2022-2025
Institute of Microbiology
2016-2025
Academy of Military Medical Sciences
2003-2025
Sichuan Center for Disease Control and Prevention
2012-2025
People's Hospital of Kaizhou District
2025
Craft Group (China)
2024
Gannan Medical University
2024
Chinese Academy of Sciences
2008-2024
Fifth Hospital of Shijiazhuang
2024
Anhui Medical University
2014-2024
Domestic dogs exhibit tremendous phenotypic diversity, including a greater variation in body size than any other terrestrial mammal. Here, we generate high density map of canine genetic by genotyping 915 from 80 domestic dog breeds, 83 wild canids, and 10 outbred African shelter across 60,968 single-nucleotide polymorphisms (SNPs). Coupling this genomic resource with external measurements breed standards individuals as well skeletal museum specimens, identify 51 regions the genome associated...
Abstract Barley ( Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by International Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report experimental computational procedures to (i) assemble more than 80,000 bacterial artificial chromosome (BAC) clones along minimum tiling path genome-wide physical map, (ii) find...
Abstract Meiotic recombination drives eukaryotic sexual reproduction and the generation of genome diversity. Tetrad analysis, which examines four chromatids resulting from a single meiosis, is an ideal method to study mechanisms homologous recombination. Here we develop isolate microspores tetrad in maize for purpose whole-genome sequencing. A high-resolution map reveals that crossovers are unevenly distributed across more likely occur genic than intergenic regions, especially common 5′-...
This study assessed the protective mechanism of astaxanthin (ASX) against ochratoxin A- (OTA-) induced cardiac injury in mice. Four groups mice were established: control group (0.1 mL olive oil + 0.1 NaHCO2), OTA 5 mg/kg body weight), ASX 100 and weight, 2 h later, weight). The test period lasted for 27 days (7 dosing, rest). Electrocardiogram, heart tissue pathology, oxidative markers (malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH)), biochemical...
Background Stimulator of interferon genes (STING) is an innate immune sensor cytoplasmic double-stranded DNA originating from microorganisms and host cells. The activation cytosolic DNA-STING pathway in tumor microenvironments usually linked to more robust adaptive responses tumors, however the intracellular function STING regulatory T cells largely unknown. In present study, we aimed explore contribution cell induction (iTreg) cervical cancer (CC) microenvironments. Methods Blood samples...
Pyrococcus furiosusArgonaute (PfAgo) emerged as a novel endonuclease for the nucleic acid test recently. However, input of exogenous guide DNA (gDNA) to activate PfAgo has reduced its flexibility. In this work, an enzyme-assisted endogenous gDNA generation-mediated target detection strategy, termed EGG-PAD, was proposed. With aid EcoR Ι, double-strand cut, producing phosphate group at 5′ end, functioning detection. The applicability assay tested in ofAlicyclobacillus acidoterrestris,...
Abstract To reveal the self-coordination mechanism of fragile ecosystem alpine tundra, we explored relationship between soil microorganisms and other elements. On tundra Changbai Mountain, different vegetation types, altitudes properties were selected as driving factors microbial community. Soil community, C- N-cycling functional fungal biomass analyzed. Structural equation model was used to study control biotic abiotic in rhizosphere The results showed that pH value had strongest direct...
Recently, genome-wide association studies (GWAS) have been reported on various pig traits. We performed a GWAS to analyze 22 traits related growth and fatness two populations: White Duroc × Erhualian F2 intercross population Chinese Sutai half-sib population.We identified 14 39 loci that displayed significant associations with at the level chromosome-wide level, respectively. The strongest was between 750 kb region SSC7 (SSC for Sus scrofa) backfat thickness first rib. This had pleiotropic...
ObjectivesShenzhen is suffering severe HIV epidemic. No systematic surveillance on high risk populations, genetic diversity, transmitted drug resistance (TDR) and molecular transmission clusters (MTCs) have been reported yet. In this study, we described them based newly diagnosed positive cases from 2011 to 2018 in Shenzhen city, China.MethodsPlasma samples of Shenzhen, China were collected 2018. The pol gene was amplified sequenced for subtyping, characterization, TDR phylogenetic analysis....
Tumor metabolic reprogramming requires high levels of adenosine triphosphate (ATP) to maintain treatment resistance, which poses major challenges chemotherapy and photothermal therapy. Especially, ATP promote copper ion efflux for limiting the curative effect cuproptosis. Here, an H
Under the background of main epidemic HIV strains (CRF01_AE and CRF07_BC) co-circulation in China, more second-generation recombinant (SGR) with CRF01_AE CRF07_BC as backbone were also emerging. In this study, we characterize a novel HIV-1 circulating form (CRF117_0107) consisting fragments from three epidemiologically unrelated HIV-1-infected individuals. One near full-length genome (NFLG) sequence was amplified, sequenced, spliced two halves using RNA extracted plasma homosexual Shenzhen,...
Human Immunodeficiency Virus (HIV) persists as a leading global health issue. A significant gap in knowledge exists our understanding of long-range interactions within the HIV-1 RNA genome. To bridge this gap, we introduce HiCapR, incorporating psoralen crosslinking proximity ligation and post-library hybridization for capturing HIV RNA-RNA interactions.Leveraging confirm presence stem structures key regions, such 5’-untranslated region(5’-UTR) Rev Response Element (RRE) stems, dimer sites...
Heterosexual transmission (HETE) represents the predominant method of for human immunodeficiency virus type 1 (HIV-1) in Shijiazhuang, Hebei Province, China. The number circulating recombinant forms (CRFs) and unique (URFs) continues to increase this region. In present study, two novel URFs (TFH010919 TFH010944) were identified, both derived from HETEs Shijiazhuang area. phylogenetic recombination breakpoint analyses conducted on near-full-length genomes revealed that CRF01_AE strains serve...
<title>Abstract</title> This study analyzed phthalates (PAEs) in Baijiu (Chinese liquor) from various cities Sichuan Province, China. The overall occurrence of PAEs was 8.66%, with five detected: Dibutyl phthalate (DBP, 75.69%), Diisobutyl (DIBP, 40.33%), Dimethyl Phthalate (DMP, 20.44%), Di (2-ethylhexyl) (DEHP, 11.05%), and Butyl benzyl (BBP, 8.29%). concentrations ranged non-detectable (ND) to 10.30 mg kg<sup>-1</sup>, DBP showing the highest mean P95 levels (0.44 1.57 kg<sup>-1</sup>). A...
Abstract To systematically analyze CT imaging features of hepatic cystic echinococcosis (CE), explore radiological-pathological correlations, and develop a diagnostic algorithm for accurate disease classification. This retrospective study included 48 pathologically confirmed cases CE from two medical centers. were analyzed by experienced radiologists, evaluating lesion characteristics including location, morphology, wall features, calcification patterns. Imaging findings correlated with...
Virion infectivity factor (Vif) is essential for the replication of human immunodeficiency virus type 1 (HIV-1) in vivo, but its function remains uncertain. Recently, we have shown that Vif proteins are able to form multimers, including dimers, trimers, or tetramers. Because multimerization required viral life cycle, propose it could be a novel target anti-HIV-1 therapeutics. Through phage peptide display method, identified set 12-mer peptides containing PXP motif binds HIV-1 protein. These...
In this study, a one-step, real-time, loop-mediated isothermal amplification (RealAmp) assay was developed, for the highly specific detection of pork DNA. For assay, mtDNA cytochrome b (cytb) gene amplified at 63 °C using SYBR Green I 45 min with Real-Time Polymerase Chain Reaction (PCR) System that measured fluorescent signal one-minute intervals. As little as 1 pg template DNA could be detected, without any cross-reactivity non-target species. Meat mixtures, heat-treated 100 15 min,...