A5100435573- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Rice Cultivation and Yield Improvement
- Research in Cotton Cultivation
- Plant-Microbe Interactions and Immunity
- Cancer, Lipids, and Metabolism
- Plant Pathogenic Bacteria Studies
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Plant Molecular Biology Research
- Atmospheric chemistry and aerosols
- Irrigation Practices and Water Management
- Traditional Chinese Medicine Analysis
- Viral gastroenteritis research and epidemiology
Czech Academy of Sciences, Institute of Biotechnology
2024-2025
Southern Regional Research Center
2017-2025
University Town of Shenzhen
2024
Tsinghua University
2024
Ningbo University
2024
Shengli Oilfield Central Hospital
2018-2024
United States Department of Agriculture
2017-2024
Agricultural Research Service
2017-2024
Temasek Life Sciences Laboratory
2024
National University of Singapore
2024
Anthrax toxin causes anthrax pathogenesis and expression levels of ANTXR2 (anthrax receptor 2) are strongly correlated with susceptibility. Previous studies found that transcript abundance varies considerably in individuals different ethnic/geographical groups, but no eQTLs (expression quantitative trait loci) have been identified. By using 3C (chromatin conformation capture), CRISPR-mediated genomic deletion dual-luciferase reporter assay, gene loci containing cis-regulatory elements were...
MicroRNAs (miRNAs) are emerging as important regulators in various pathobiological processes cancer. Genistein, a major isoflavonoid isolated from dietary soybean, possesses wide variety of biological activities particularly cancer prevention. However, the molecular mechanisms by which genistein elicits its effects on ovarian cells have not been fully elucidated. In this study, we reported that expression miR-27a was higher human relative to benign tissues. Meanwhile, transfection SKOV3 with...
Abnormalities in signal transducer and activator of transcription 3 (STAT3) are involved the oncogenesis oral squamous cell carcinoma (OSCC). MicroRNA-21 (miR-21) is an important gene expression regulator to OSCC. miR-21 induction by STAT3 has been reported multiple human cancers. In present study, we found that (-/p) was positively correlated with 60 OSCC samples. A reporter assay showed overexpression dependent on activation. WP1066, a small molecular inhibitor STAT3, used suppress cells....
Abstract The dynamics of SARS-CoV-2 RNA structure and their functional relevance are largely unknown. Here we develop a simplified SPLASH assay comprehensively map the in vivo RNA-RNA interactome genome across viral life cycle. We report canonical alternative structures including 5′-UTR 3′-UTR, frameshifting element (FSE) pseudoknot cyclization both cells virions. provide direct evidence interactions between Transcription Regulating Sequences, which facilitate discontinuous transcription. In...
Deregulation of microRNAs (miRNAs) is implicated in tumor progression. We attempt to identify the association between miR-138 and Sentrin/SUMO-specific protease 1 (SENP1) as a radiosensitization-related gene characterize biological function by which SENP1 was regulated influence radiosensitization lung cancer cells. In this study, we showed that miRNA-138 reduced both clinical specimens cell lines effective inhibit expression. Moreover, high levels are associated with lower proliferation...
Actin polymerizes to form part of the cytoskeleton and organize polar growth in all eukaryotic cells. Species with numerous actin genes are especially useful for dissection molecular function due redundancy neofunctionalization. Here, we investigated role a cotton (Gossypium hirsutum) gene organization filaments lobed cotyledon pavement cells highly elongated single-celled trichomes that comprise lint fibers. Using mapping-by-sequencing, virus-induced silencing, modeling, identified...
Mammalian circadian rhythm is established by the negative feedback loops consisting of a set clock genes, which lead to expression thousands downstream genes in vivo. As genome-wide transcription organized under high-order chromosome structure, it largely uncharted how gene influenced architecture. We focus on function chromatin structure proteins cohesin as well CTCF (CCCTC-binding factor) rhythm. Using circular conformation capture sequencing, we systematically examined interacting loci...
Human Immunodeficiency Virus (HIV) persists as a leading global health issue. A significant gap in knowledge exists our understanding of long-range interactions within the HIV-1 RNA genome. To bridge this gap, we introduce HiCapR, incorporating psoralen crosslinking proximity ligation and post-library hybridization for capturing HIV RNA-RNA interactions.Leveraging confirm presence stem structures key regions, such 5’-untranslated region(5’-UTR) Rev Response Element (RRE) stems, dimer sites...
Drought is an important environmental stress limiting wheat ( Triticum aestivum L.) productivity in water limited regions. Our aim was to understand the relationships between target agronomic traits and grain yield (GY) responses drought, prioritize genotypes for high under different conditions. Thirty spring were evaluated over 2009 2010 GY T1 (non‐irrigated), T2 (50% evapotranspiration [ET] irrigated), T3 (100% ET irrigated) irrigation regimes. caused noticeable fewer days physiological...
Circadian rhythm exerts its influence on animal physiology and behavior by regulating gene expression at various levels. Here we systematically explored circadian long non-coding RNAs (lncRNAs) in mouse liver examined their regulation. We found that a significant proportion of lncRNAs are expressed enhancer regions, mostly bound two key transcription factors, BMAL1 REV-ERBα. These showed similar phases with nearby genes. The extent nuclear localization is higher than protein coding genes but...
MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in the carcinogenesis and progression of cancers. Aberrant expression miRNAs tissue plasma has been found various solid tumors. Our research aims to determine whether abnormal miRNA patterns can be used as a predictive marker for diagnosis prognosis small cell lung cancer (SCLC). Fifty SCLC patients 30 healthy controls annotated with clinical characteristics specific questionnaire survey smoking history were available....
Abstract Altered metabolism is considered as a hallmark of cancer. Here we investigated expression 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) 2 lyase (HMGCL), an essential enzyme in ketogenesis, which produces ketone bodies by the breakdown fatty acids to supply energy, nasopharyngeal carcinoma (NPC). The HMGCL was silenced NPC tissue. Downregulation associated with low intracellular β-hydroxybutyrate (β-HB) production, thereby reducing reactive oxygen species (ROS) generation. Ectopic...
Endothelial inflammation and dysfunction are critical to the process of atherosclerosis. Emerging evidence demonstrates that upregulation miR-200a reduces VCAM-1 expression prevents monocytic cell adhesion onto aortic endothelium. However, limited information is available about role microRNA-200a (miR-200a) in facilitating atherosclerotic lesion formation. We investigated anti-inflammatory anti-atherosclerotic actions miR-200a. Human umbilical vein endothelial cells (HUVECs) were cultured...
Abstract Phosphoglycerate mutase 1 (PGAM1) is a recently identified key catalytic enzyme in aerobic glycolysis. Recent literature has documented that dysregulated PGAM1 expression associated with tumorigenesis various cancers. However, the status and biological function of non-small-cell lung cancer (NSCLC) are poorly elucidated. In this study, we found was overexpressed NSCLC tissues high poor prognosis patients. Functionally, gain- loss-of-function analysis showed promoted proliferation...
Virus-induced genomic remodeling and altered gene expression contribute significantly to cancer development. Some oncogenic viruses such as Human papillomavirus (HPV) specifically trigger certain cancers by integrating into the host’s DNA, disrupting regulation linked cell growth migration. The effect can be through direct integration of viral genomes host genome or indirect modulation pathways/proteins proteins. Viral proteins also disrupt key cellular processes like apoptosis DNA repair...
Abstract Background Lettuce ( Lactuca sativa L. ) is an economically important vegetable crop worldwide. believed to be domesticated from a single wild ancestor serriola and subsequently diverged into two major morphologically distinct types: leafy lettuce stem lettuce. However, the role of epigenetic variation in domestication divergence remains largely unknown. Results To understand genetic basis underlying divergence, we generate single-base resolution DNA methylomes 52 accessions,...
Bulbus Fritillariae is the most commonly used antitussive herb in China. Eleven species of Fritillaria are recorded as Chinese Pharmacopoeia. Cirrhosae a group six with higher efficiency and lower toxicity derived mainly from wild sources. Because their market price, five other often sold deceptively herbal market. To ensure efficacy safety medicinal herbs, authentication botanical resources first step quality control. Here, DNA based identification method was developed to authenticate...
Glioblastoma (GBM) is the most common type of malignant brain tumor. In spite recent advancements in surgical techniques, chemotherapy, and radiation therapy, patients with GBM often face a dire prognosis. MicroRNAs have been shown to modulate aggressiveness various cancers, emerged as possible therapeutic agents for management GBM. miR‑205 dysregulated glioma act prognostic indicator. However, role development has not elucidated. To better understand pathogenesis GBM, we examine biological...