A5035758123- Reproductive tract infections research
- Gut microbiota and health
- Urinary Tract Infections Management
- Adolescent Sexual and Reproductive Health
- Reproductive System and Pregnancy
- Clostridium difficile and Clostridium perfringens research
- Hepatitis B Virus Studies
- Probiotics and Fermented Foods
- Inflammatory Bowel Disease
- Immune Cell Function and Interaction
- Syphilis Diagnosis and Treatment
- Hepatitis C virus research
- Cervical Cancer and HPV Research
- Appendicitis Diagnosis and Management
- Tuberculosis Research and Epidemiology
- HIV, Drug Use, Sexual Risk
- Diagnosis and treatment of tuberculosis
- Inflammatory Myopathies and Dermatomyositis
- Health and Medical Research Impacts
- Plant Virus Research Studies
- Liver Disease Diagnosis and Treatment
- Gastrointestinal motility and disorders
- COVID-19 Clinical Research Studies
- Global Health and Surgery
- Advances in Oncology and Radiotherapy
Harvard University
2020-2024
Ragon Institute of MGH, MIT and Harvard
2020-2024
Lemuel Shattuck Hospital
2021-2023
Massachusetts General Hospital
2020-2023
University College Hospital
2012
University College London
2012
Washington University in St. Louis
2006-2011
Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance Lactobacillus iners instead of crispatus, which has more beneficial associations. Strategies to L. crispatus and inhibit are thus needed. We show that oleic acid (OA) similar long-chain fatty acids simultaneously enhance growth. These...
Background The constellation of human inflammatory bowel disease (IBD) includes ulcerative colitis and Crohn's disease, which both display a wide spectrum in the severity pathology. One theory is that multiple genetic hits to host immune system may contribute susceptibility IBD. However, experimental proof this concept still lacking. Several mouse models each recapitulate some aspects IBD have utilized single gene defect induce colitis. none produced pathology clearly distinguishable as...
Bacterial vaginosis (BV), a condition in which the vaginal microbiota consists of community obligate and facultative anaerobes rather than dominated by single species Lactobacillus, affects ~30% women US. Women with BV are at 60% increased risk for HIV acquisition 3-times more likely to transmit an uninfected partner. As cervicovaginal mucus (CVM) is first line defense against mucosal pathogens home resident microbiota, we hypothesized barrier function CVM may be diminished BV. Here,...
Abstract Background Cervicovaginal bacterial communities composed of diverse anaerobes with low Lactobacillus abundance are associated poor reproductive outcomes such as preterm birth, infertility, cervicitis, and risk sexually transmitted infections (STIs), including human immunodeficiency virus (HIV). Women in sub-Saharan Africa have a higher prevalence these high-risk when compared to Western populations. However, the transition cervicovaginal between high- low-risk community states over...
Abstract Bacterial vaginosis (BV), the overgrowth of diverse anaerobic bacteria in vagina, is most common cause vaginal symptoms worldwide. BV frequently recurs after antibiotic therapy, and best probiotic treatments only result transient changes from BV-associated states to “optimal” communities dominated by a single species Lactobacillus . Therefore, additional treatment strategies are needed durably alter microbiota composition for patients with BV. Vaginal transplantation (VMT), transfer...
Diverse and non-Lactobacillus-dominated vaginal microbial communities are associated with adverse health outcomes such as preterm birth the acquisition of sexually transmitted infections. Despite importance recognizing understanding key risk-associated features these communities, their heterogeneous structure properties remain ill-defined. Clustering approaches commonly used to characterize but they lack sensitivity robustness in resolving substructures revealing transitions between...
Abstract Background Chromosome four of Drosophila melanogaster , known as the dot chromosome, is largely heterochromatic, shown by immunofluorescent staining with antibodies to heterochromatin protein 1 (HP1) and histone H3K9me. In contrast, absence HP1 H3K9me from chromosome in D. virilis suggests that this region euchromatic. diverged 40 60 million years ago. Results Here we describe finished sequencing analysis 11 fosmids hybridizing (372,650 base-pairs) seven major euchromatic arms...
The microbiome of the female genital tract (FGT) is closely linked to reproductive health outcomes. Diverse, anaerobe-dominated communities with low Lactobacillus abundance are associated a number adverse outcomes, such as preterm birth, cervical dysplasia, and sexually transmitted infections (STIs), including HIV. Vaginal dysbiosis local mucosal inflammation, which likely serves biological mediator poor Yet precise mechanisms this FGT inflammation remain unclear. Studies in humans have been...
Viral hepatitis is a major cause of death in Africa. A paucity community-based studies on the determinants viral Africa impedes efforts to appropriately target prevention, screening, and treatment interventions. We assessed factors associated with B virus (HBV) C (HCV) semi-urban health district Cameroon.We conducted community-based, cross-sectional study from 28 February 15 May 2021 Buea Health District Buea, Cameroon. recruited sample consenting individuals aged over years old....
Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus -deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance iners instead of crispatus , which has more beneficial associations. Strategies to L. and inhibit are thus needed. We show that oleic acid (OA) similar long-chain fatty acids simultaneously enhance growth. These phenotypes require...
Abstract Diverse and non- Lactobacillus -dominated vaginal microbial communities are associated with adverse health outcomes such as preterm birth the acquisition of sexually transmitted infections. Despite importance recognizing understanding key risk-associated features these communities, their heterogeneous structure properties remain ill-defined. Clustering approaches commonly used to characterize but they lack sensitivity robustness in resolving substructures revealing transitions...
Vaginal microbiota composition affects several important reproductive health outcomes. Lactobacillus crispatus-dominant bacterial communities have favorable associations whereas anaerobe-dominant deficient of lactobacilli are linked to poor outcomes, including vaginosis (BV). iners, the most abundant vaginal species worldwide, has adverse compared L. crispatus, but standard metronidazole treatment for BV promotes iners-dominance, likely contributing post-treatment relapse. iners is...
Abstract Reported adverse reactions to the mRNA-1273 vaccine (Spikevax, Moderna Inc) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) range from mild, local delayed cutaneous rarer, more serious such as myocarditis. Here, we describe presentation and successful treatment of delayed, localized necrotizing inflammatory myositis following a third dose SARS-CoV-2 vaccine. To our knowledge, this is first report biopsy-confirmed, after administration an booster.
<h3>Introduction</h3> Crohn9s disease management requires knowledge of overall burden. A new MRI score activity was tested against reference standards global activity—Harvey Bradshaw index (HBI) and faecal calprotectin. <h3>Methods</h3> 34 patients (15 male) median age 33 (range 17–78) with known or suspected underwent MR enterography (axial/coronal HASTE, TrueFisp post gadolinium coronal VIBE/THRIVE at 1.5T (n=24) 3T (n=10)). Same day HBI questionnaire calprotectin were measured. Two...
Summary Research training is a core component of medical education, but many African schools lack resources to support student research, impeding global health progress. Conferences offer alternate venues learn about network, and receive scientific feedback, conference access for trainees limited. We hypothesized that research mentorship symposium could promote knowledge interest among attendees. Methods co-organized attended by students early career doctors from institutions in-person...
Abstract Background Cervicovaginal microbiota domination by Lactobacillus crispatus is associated with beneficial health outcomes, whereas L. iners dominance has more adverse associations. However bacterial vaginosis (BV) treatment metronidazole (MTZ) typically leads to rather than crispatus. differs from other lactobacilli its inability grow in MRS media. We hypothesized that exploring this growth difference would identify targets for selective inhibition. Methods Bacteria were grown...