A5065442564- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Congenital heart defects research
- Mesenchymal stem cell research
- Viral Infections and Immunology Research
- Amino Acid Enzymes and Metabolism
- Photoreceptor and optogenetics research
- Developmental Biology and Gene Regulation
- Plant and Fungal Interactions Research
- RNA modifications and cancer
- interferon and immune responses
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Cardiovascular Function and Risk Factors
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Respiratory viral infections research
Sree Chitra Thirunal Institute for Medical Sciences and Technology
2019-2024
RIKEN Center for Integrative Medical Sciences
2019-2022
Hebrew University of Jerusalem
2017-2020
Rajiv Gandhi Centre for Biotechnology
2009-2017
CLS Technology (Norway)
2017
Johns Hopkins University
2013
University of California, Riverside
2011-2012
University of Delaware
2011
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in mammalian genomes, and yet, their functions remain largely unknown. As part FANTOM6 project, we systematically knocked down expression 285 lncRNAs human dermal fibroblasts quantified cellular growth, morphological changes, transcriptomic responses using Capped Analysis Gene Expression (CAGE). Antisense oligonucleotides targeting same exhibited global concordance, molecular phenotype, measured by CAGE, recapitulated...
Abstract Introduction Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are self-renewing multipotent progenitors with the potential to differentiate into multiple lineages of mesoderm, in addition generating ectodermal and endodermal by crossing germline barrier. In present study we have investigated ability UCB-MSCs generate neurons, since were able observe varying degrees neuronal differentiation from a few batches very simple induction protocols whereas other required...
Within the scope of FANTOM6 consortium, we perform a large-scale knockdown 200 long non-coding RNAs (lncRNAs) in human induced pluripotent stem cells (iPSCs) and systematically characterize their roles self-renewal pluripotency. We find 36 lncRNAs (18%) exhibiting cell growth inhibition. From 123 with transcriptome profiling, (29.3%) show molecular phenotypes. Integrating phenotypes chromatin-interaction assays further reveals cis- trans-interacting partners as potential primary targets....
Hes-1 and Hes-5 are downstream effectors of Notch signaling that known to be involved in different aspects neural stem cell proliferation differentiation. Evidence has emerged expression can regulated by alternate pathways independent canonical Notch/CBF1 interaction. This context-dependent differential regulation progenitor gains a lot importance as it would help its exponential expansion without the requirement interaction from neighboring cells during development. Here, we have clearly...
Retinal ganglion cells (RGC) transplantation is a promising strategy to restore visual function resulting from irreversible RGC degeneration occurring in glaucoma or inherited optic neuropathies. We previously demonstrated FGF2 induced differentiation of mouse embryonic stem (ESC) lineage, capable retinal Ganglion Cell Layer (GCL) integration upon mice. Here, we evaluated possible improvement by ES cell derived neural progenitors depleted mice models. ESC (ES-NP) were transplanted into NMDA...
Histone variants and their chaperones are key regulators of eukaryotic transcription, critical for normal development. The histone variant H3.3 has been shown to play important roles in pluripotency differentiation, although its genome-wide patterns have investigated, little is known about the role dynamic turnover transcriptional regulation. To elucidate dynamics embryonic stem cell (ESC) biology, we generated mouse ESC lines carrying a single copy doxycycline (Dox)-inducible HA-tagged...
Electrically conducting polymers are prospective candidates as active substrates for the development of neuroprosthetic devices. The utility these promoting differentiation embryonic stem cells paves viable routes regenerative medicine. Here, we have tuned electrical and mechanical cues provided to during by precisely straining polymer (CP) coated, elastomeric-substrate. Upon substrates, neural pattern occurs in form aggregates, accompanied a gradient where substrate interface reveals higher...
ABSTRACT Differential regulation of Brn3b is essential for the Retinal Ganglion Cell (RGC) development in two phases retinal histogenesis. This biphasic required first, during early histogenesis RGC fate specification and secondly, late histogenesis, where needed axon guidance survival. Here, we have looked into how at these stages happens. We identified miRNAs, miR‐23a miR‐374, as regulators expression, stage development. Temporal expression pattern E10–19, PN1–7, adult retina revealed an...
Abstract Homeobox gene Tlx3 is known to promote glutamatergic differentiation and expressed in post-mitotic neurons of CNS. Contrary this here, we discovered that the proliferating progenitors external granule layer cerebellum, examined factors regulate expression. Using Pax6 −/− Sey mouse model molecular interaction studies demonstrate a key activator specifically induces its expression starting at embryonic day (E)15. By Postnatal (PN)7, highly restricted manner cerebellar posterior lobes,...
Abstract Long non-coding RNAs (lncRNAs) constitute the majority of transcripts in mammalian genomes and yet, their functions remain largely unknown. We systematically knockdown 285 lncRNAs expression human dermal fibroblasts quantified cellular growth, morphological changes, transcriptomic responses using Capped Analysis Gene Expression (CAGE). Antisense oligonucleotides targeting same lncRNA exhibited global concordance, molecular phenotype, measured by CAGE, recapitulated observed...
Cellular reprogramming is driven by a defined set of transcription factors; however, the regulatory logic that underlies cell-type specification and diversification remains elusive. Single-cell RNA-seq provides unprecedented coverage to measure dynamic molecular changes at single-cell resolution. Here, we multiplex ectopically express 20 pro-neuronal factors in human dermal fibroblasts demonstrate widespread neurons based on cell morphology canonical neuronal marker expressions. analysis...
Rapid and efficient detection of viral infection is crucial for the prevention disease spread during an outbreak timely clinical management. In this paper, utility Tat peptide-modified molecular beacons (MBs) as a rapid diagnostic tool virus-infected cells was demonstrated. The intracellular delivery mediated by peptide enabled infected within 30 s, reaching saturation in signal min. This scheme coupled with flow cytometry (FC), resulting automated, high-throughput method identification...
Endothelial progenitor cells (EPCs) play a significant role in multiple biological processes such as vascular homeostasis, regeneration, and tumor angiogenesis. This makes them promising cell of choice for studying variety processes, toxicity assays, biomaterial-cell interaction studies, well tissue-engineering applications. In this study, we report the generation two clones SV40-immortalized EPCs from umbilical cord blood. These retained most functional features mature endothelial showed no...
Abstract Collagen crosslinking, mediated by lysyl oxidase, is an adaptive mechanism of the cardiac repair process initiated fibroblasts postmyocardial injury. However, excessive crosslinking leads to wall stiffening, which impairs contractile properties left ventricle and heart failure. In this study, we investigated role periostin, a matricellular protein, in regulation oxidase response angiotensin II TGFβ1. Our results indicated that periostin silencing abolished TGFβ1‐mediated...
Summary The conversion of cell fates is controlled by hierarchical gene regulatory networks (GRNs) that induce remarkable alterations in cellular and transcriptome states. identification key regulators within these from myriad candidate genes, however, poses a major research challenge. Here we present Convert-seq, combining single-cell RNA sequencing (scRNA-seq) pooled ectopic expression with new strategy to discriminate reads derived exogenous endogenous transcripts. We demonstrate...
Summary Within the scope of FANTOM6 consortium, we perform a large-scale knockdown 200 long non-coding RNAs (lncRNAs) in human induced pluripotent stem (iPS) cells, and systematically characterize their roles self-renewal pluripotency. We find 36 lncRNAs (18%) exhibiting cell growth inhibition From 123 with transcriptome profiling, (29.3%) show molecular phenotypes. Integrating phenotypes chromatin-interaction assays further reveals cis - trans -interacting partners as potential primary...