A5069348272- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Pluripotent Stem Cells Research
- Inflammatory mediators and NSAID effects
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Melanoma and MAPK Pathways
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Single-cell and spatial transcriptomics
- Nerve injury and regeneration
- Neonatal Respiratory Health Research
- Wound Healing and Treatments
- Ion channel regulation and function
- Tryptophan and brain disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Peripheral Neuropathies and Disorders
- Autoimmune Neurological Disorders and Treatments
- Connective tissue disorders research
- Neurogenesis and neuroplasticity mechanisms
- Wnt/β-catenin signaling in development and cancer
- Fibroblast Growth Factor Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Bone and Dental Protein Studies
RIKEN Center for Integrative Medical Sciences
2018-2024
Nihon University
2013-2022
Osaka Medical and Pharmaceutical University
1958
Abstract Small, soluble metabolites not only are essential intermediates in intracellular biochemical processes, but can also influence neighbouring cells when released into the extracellular milieu 1–3 . Here we identify metabolite and neurotransmitter GABA as a candidate signalling molecule synthesized secreted by activated B plasma cells. We show that cell-derived promotes monocyte differentiation anti-inflammatory macrophages secrete interleukin-10 inhibit CD8 + T cell killer function....
Tumor necrosis factor α (TNF-α) induces the expression and secretion of interleukin 8 (IL-8), which contributes to synovitis in rheumatoid arthritis (RA). To elucidate mechanism onset RA, we used synovial fibroblasts without autoimmune inflammatory diseases investigated MAPK signaling pathways TNF-α-induced IL-8 expression. Synovial isolated from healthy dogs were characterized by flow cytometry, positive for fibroblast markers CD29, CD44, CD90 but negative hematopoietic cell CD14, CD34,...
Abstract The proinflammatory cytokine interleukin 1β (IL-1β) induces prostaglandin E 2 (PGE ) production via upregulation of cyclooxygenase-2 (COX-2) expression in synovial fibroblasts. This effect IL-1β is involved osteoarthritis. We investigated MAPK signaling pathways IL-1β-induced COX-2 feline In the presence inhibitors, and PGE release were both attenuated. induced phosphorylation p38, JNK, MEK, ERK1/2. A JNK inhibitor prevented not only but also MEK ERK1/2 IL-1β-stimulated cells,...
Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of immune response and inflammation. In this study, we investigated effect proinflammatory interleukin-1β (IL-1β) on IL-6 expression canine dermal fibroblasts. IL-1β induced mRNA protein release time- dose-dependent manner. When cells were treated with inhibitors mitogen-activated kinases (MAPKs), extracellular signal-regulated kinase (ERK) inhibitor FR180240 inhibited IL-1β-induced expression, but not SP600125 or...
Abstract Cancer-promoting inflammation is an important event in cancer development. Canine urothelial carcinoma (cUC) overexpresses prostaglandin E 2 (PGE ) and has a unique sensitivity to cyclooxygenase (COX2)-inhibiting therapy. In addition, majority of cUC harbour BRAF V595E mutation. However, mechanisms underlying aberrant PGE production patients remain unclear. Drug screening revealed that inhibition RAF/MEK/ERK pathway, p38 JNK pathway reduced cells. By pharmacological the multiple...
Cardiac fibroblasts participate in the inflammatory process of heart diseases as sentinel cells cardiac tissue. In this study, we investigated effect proinflammatory cytokine, interleukin 1β (IL-1β), on expression 8 (IL-8), which contributes to induction innate immunity via activation and recruitment immune cells, such neutrophils, site inflammation canine fibroblasts. IL-1β mediates IL-8 mRNA protein release a dose- time-dependent manner. The IL-β-mediated were inhibited by...
We investigated the in vitro differentiation of canine bone marrow stromal cells (BMSCs) into voltage- and glutamate-responsive neuron-like cells. BMSCs were obtained from healthy beagle dogs. Canine incubated with basal medium for neurons containing recombinant human basic fibroblast growth factor (bFGF; 100 ng/ml). The viability bFGF-treated was assessed by a trypan blue exclusion assay, morphology monitored. Real-time RT-PCR performed to evaluate mRNA expression neuronal, neural stem cell...
Matrix metalloproteinases (MMPs) play a pivotal role in tissue remodeling by degrading the extracellular matrix (ECM) components. This mechanism is implicated variety of physiological and pathological cellular processes including wound healing. One key proteins involved this process proinflammatory cytokine interleukin-1β (IL-1β, which induces expression MMP-3 mRNA secretion protein dermal fibroblasts. In study, we first investigated contribution activating transcription factor 2 (ATF-2) to...
The pro-inflammatory cytokine interleukin 1β (IL-1β) induces the synthesis of prostaglandin E2 by upregulating cyclooxygenase-2 (COX-2) in synovial tissue individuals with autoimmune diseases, such as rheumatoid arthritis (RA). IL-1β-mediated stimulation NF-κB and MAPK signaling is important for pathogenesis RA; however, crosstalk(s) between remains to be understood. In this study, we established a model IL-1β-induced synovitis investigated role synovitis. We observed an increase mRNA...
Bone marrow stromal cells (BMSCs) are considered as candidates for regenerative therapy and a useful model studying neuronal differentiation. The role of basic fibroblast growth factor (bFGF) in differentiation has been previously studied; however, the signaling pathway involved this process remains poorly understood. In study, we investigated bFGF-induced canine BMSCs. bFGF induced mRNA expression neuron marker, microtubule associated protein-2 (MAP2) neuron-like morphological change...
Inflammatory and microenvironmental factors produced by cancer cells are thought to directly or indirectly promote cell growth. Prostaglandins, including prostaglandin E2, have key roles as a microenvironment factor in influencing the development of tumors, rate limiting enzyme cyclooxygenase 2 (COX-2). In this study, we used canine melanoma treated with proinflammatory cytokine interleukin 1β (IL-1β) investigated transcriptional nuclear factor-κB (NF-κB) signaling IL-1β-induced COX-2...
Acute kidney injury (AKI) is characterized by a sudden loss of renal function. Early recognition AKI, especially in critically ill patients, essential for adequate therapy. Currently, neutrophil gelatinase-associated lipocalin (NGAL) considered to be an effective biomarker AKI; however, the regulation its expression and function tubular cells remains unclear. In this study, we investigated NGAL IL-1β-treated Madin-Darby canine (MDCK) as model cells. IL-1β induced disturbance localization...
We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, microstructures neuron-like after were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, 3.8 2.1 × 106 collected in one passage. Approximately 65% changed morphology differentiation, nearly all stained positive against neuron-specific enolase. such as cellular organelles, filaments growth cones these bore close...
The proinflammatory mediator bradykinin stimulated cyclooxygenase-2 (COX-2) expression and subsequently prostaglandin E2 synthesis in dermal fibroblasts. involvement of B2 receptors Gαq the role was suggested by using pharmacological inhibitors. PKC activator PMA COX-2 mRNA expression. Bradykinin failed to induce presence inhibitors, whereas effect observed absence extracellular Ca2+. Bradykinin-induced inhibited cells transfected with PKCε siRNA. These observations suggest that novel is...
We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO FBS (DF group) or without (DF-free group); fresh used as a control. The viability similar in DF-free control groups, while those DF group lower. In all differentiated into neuron-like that stained positive against neuron markers, mRNA expression levels markers...
Cellular reprogramming is driven by a defined set of transcription factors; however, the regulatory logic that underlies cell-type specification and diversification remains elusive. Single-cell RNA-seq provides unprecedented coverage to measure dynamic molecular changes at single-cell resolution. Here, we multiplex ectopically express 20 pro-neuronal factors in human dermal fibroblasts demonstrate widespread neurons based on cell morphology canonical neuronal marker expressions. analysis...
To investigate the in vitro differentiation of canine bone marrow stromal cells (BMSCs) into functional, mature neurons.Bone from 6 adult dogs.BMSCs were isolated and chemically induced to develop neurons. The morphology BMSCs during neuronal induction was monitored, immunocytochemical analyses for neuron markers performed after induction. Real-time PCR methods used evaluate mRNA expression levels neural stem or progenitor cells, neurons, ion channels, western blotting assess proteins before...
In autoimmune diseases, fibroblasts produce and secrete various cytokines act as sentinel immune cells during inflammatory states. However, the contribution of (i.e. dermal fibroblasts) in diseases skin, such atopic dermatitis, has been obscure. The pro-inflammatory cytokine interleukin 1β (IL-1β) induces expression chemokines, 8 (IL-8), skin. IL-8 activation recruitment innate neutrophils to site inflammation. IL-1β-mediated induction is important for pathogenesis diseases; however,...
The specification of cell identity depends on the exposure cells to sequences bioactive ligands. All-trans retinoic acid (ATRA) affects neuronal development in early stage, and it is involved lineage reprogramming. We previously established a fibroblast-like dedifferentiated fat (DFATs) derived from highly homogeneous mature adipocytes, which are more suitable for study cellular Canine cognitive dysfunction similar human dysfunction, suggesting that dogs could be pathological pharmacological...
The rate of glucose uptake dramatically increases in cancer cells even the presence oxygen and fully functioning mitochondria. Cancer produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as “Warburg effect.” In present study, we treated canine melanoma with analog 2-deoxy-D-glucose (2-DG) investigated its effect on cell growth. 2-DG attenuated growth time- dose-dependent manner. Cell was also inhibited following treatment transporter...
Summary The conversion of cell fates is controlled by hierarchical gene regulatory networks (GRNs) that induce remarkable alterations in cellular and transcriptome states. identification key regulators within these from myriad candidate genes, however, poses a major research challenge. Here we present Convert-seq, combining single-cell RNA sequencing (scRNA-seq) pooled ectopic expression with new strategy to discriminate reads derived exogenous endogenous transcripts. We demonstrate...