A5101781704- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Urticaria and Related Conditions
- Cancer, Stress, Anesthesia, and Immune Response
- Medicinal Plant Pharmacodynamics Research
- Cellular Mechanics and Interactions
- Nonmelanoma Skin Cancer Studies
- Toxin Mechanisms and Immunotoxins
- HER2/EGFR in Cancer Research
- Cutaneous lymphoproliferative disorders research
- Herbal Medicine Research Studies
- CAR-T cell therapy research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
Kyoto University
2017-2025
Osaka University
1985-2008
RIKEN Center for Integrative Medical Sciences
2008
University of Washington Medical Center
1995
University of Alabama at Birmingham
1991
Institut de Biologie Moléculaire et Cellulaire
1989
New York University
1988
Tokyo University of Science
1988
Kanazawa Medical University
1988
University Medical Center Freiburg
1988
Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column then a Mono P with fast protein liquid reversed-phase HPLC. A 5300-fold enrichment in specific activity BCDF about 25% recovery attained. The evidenced the following: (i) 1.7 X 10(7) units/mg protein, (ii) only two bands, Mr 19,000 21,000, were identified NaDodSO4/PAGE under reduced as well nonreduced conditions,...
Growth and differentiation of thymocytes mature T lymphocytes is regulated by cellular interactions that are in part mediated soluble factors. We identify IL-6, formerly called B cell stimulating factor (BSF-2). IFN-beta 2, or hybridoma-plasmacytoma growth (HPGF) as a novel costimulant rIL-6 induced six-to seven-fold increase proliferation human stimulated with suboptimal doses PHA. A similar effect added IL-6 could be observed using peripheral blood lymphocytes, but only if the cultures...
Abstract Small, soluble metabolites not only are essential intermediates in intracellular biochemical processes, but can also influence neighbouring cells when released into the extracellular milieu 1–3 . Here we identify metabolite and neurotransmitter GABA as a candidate signalling molecule synthesized secreted by activated B plasma cells. We show that cell-derived promotes monocyte differentiation anti-inflammatory macrophages secrete interleukin-10 inhibit CD8 + T cell killer function....
BACKGROUNDPrecise stratification of patients with non-small cell lung cancer (NSCLC) is needed for appropriate application PD-1/PD-L1 blockade therapy.METHODSWe measured soluble forms the immune-checkpoint molecules PD-L1, PD-1, and CTLA-4 in plasma advanced NSCLC before blockade. A prospective biomarker-finding trial (cohort A) included 50 previously treated who received nivolumab. retrospective observational study was performed any therapy (cohorts B C), cytotoxic chemotherapy D), or...
Polyclonal B cell activation is commonly observed in AIDS and infection with HIV. The effect of HIV on the induction stimulatory factor 2 (BSF-2) production was examined, since BSF-2 plays an essential role differentiation activated cells to Ig-secreting cells. Increased mRNA levels increased secretion were soon after exposure mononuclear isolated from healthy donors both "live" inactivated HIV-induced seen monocyte/macrophages, but not T These results suggest that overproduction might...
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on variety cells other than cells, and moreover, expression mRNA detected in interleukin-1 beta-stimulated glioblastoma astrocytoma cell lines. Here, we studied function pheochromocytoma PC12 model system for induction neuronal differentiation. possess specific receptors BSF-2. The BSF-2-stimulated expressed c-fos proto-oncogene transiently, they began to change morphologically...
Past studies have shown that freshly isolated human B cells from peripheral blood and tonsils do not express IL-6 receptors (IL-6R); however, mitogen or antigen activation of these induces IL-6R responsiveness to IL-6. In this study, we a high proportion enzymatically dissociated appendix, gut-associated lymphoreticular tissue (GALT), expresses the IL-6R, recombinant significant increases in number Ig-producing cells. The IL-6-induced increase is restricted IgA isotype. Further, IgA2 major...
Recombinant human interleukin 6 (IL-6), also termed B-cell-stimulatory factor 2 (BSF-2) or interferon-beta 2, was found to stimulate the proliferation of mouse thymocytes costimulated with phytohemagglutinin (PHA). In addition, IL-6 synergistically enhanced stimulation thymocyte by recombinant 1 (IL-1) (IL-2). Mature lacking peanut agglutinin receptor are main target action. Incubation in presence PHA resulted an increased expression IL-2 (IL-2R) as demonstrated flow cytometry. Monoclonal...
At least two distinct B cell stimulatory factors (BSF) were found to be involved in the differentiation of Staphylococcus aureus Cowan I (SAC)-stimulated human cells IgG-producing cells. A factor tentatively called (BCDF I) was one fraction, and a second factor, BCDF II another fraction. The fraction alone induces IgG-production SAC-stimulated cells, but does not. enhances IgG production presence Studies concerning time-course action revealed that it contains late-acting acts on most...
Abstract Human peripheral T cells were transformed by human cell leukemia virus (HTLV), and lines producing BGDF (BCGF II) BCDF established. Among these lines, a line, TCL-Na1, secreted the highest level of both BCDF, amount TCL-Na1 was 900-fold more than that produced PHA-stimulated cells. Within limits our examination, none HTLV-transformed IL 2 or BSF-p1 I). had m.w. 35,000 pI value 5.5, being separated from BGDF, which eluted in fractions corresponding to 60,000 values 5 6. induced...
The geometric organization of collagen fibers in human reticular dermis and its relationship to that elastic remain unclear. tight packing complex intertwining dermal hinder accurate analysis fiber orientation. We hypothesized combined multiphoton microscopy biaxial extension could overcome this issue. Continuous observation fresh sheets under revealed the geometry network is maintained during expansion. Full-thickness thigh skin samples were biaxially extended cleared visualize entire...
Combination treatment consisting of IL-2 together with anti-IL-2 mAbs results in markedly larger increases the numbers CD8+ T cells, dendritic cells (DCs) and NK vivo compared observed injections or antibodies alone. We previously showed that this combination overcomes problems associated short half-life vivo. Importantly, but not alone protected mice against tumor metastases lungs. Here we have investigated which cell types are responsible for protective immunity tumors. analyzed were...
Despite the success of cancer immunotherapies such as programmed cell death-1 (PD-1) and PD-1 ligand 1 (PD-L1) inhibitors, patients often develop resistance. New combination therapies with PD-1/PD-L1 inhibitors are needed to overcome this issue. Bezafibrate, a peroxisome proliferator-activated receptor-γ coactivator 1α/peroxisome receptor complexes, has shown synergistic antitumor effect blockade in mice that is mediated by activation mitochondria T cells. We have therefore now performed...
Staphylococcus aureus Cowan I (SAC) induced proliferation of human B cells but did not lead the to differentiate immunoglobulin (Ig)-producing cells. The differentiation SAC-stimulated IgG-producing was totally dependent on T or cell factor, designated factor (BCDF I). BCDF exerted its effect between 48 and 72 hr after initiation culture, at which time 3H-TdR uptake had already reached a maximal level. Highly purified IL 2 induce for Therefore differentiation-inducing ability due residual in...
Previously, we described the characteristics of a cell line that is derived from low density fraction human tonsillar cells and, on basis number criteria, related to follicular dendritic (FDC). This line, FDC-1, binds B lymphocytes and not T lymphocytes, promotes anti-Ig- or anti-CD40-induced proliferation. In this work, show culturing with small numbers FDC-1 leads significant production IL-6 both IgM IgG. As few as 50 100 augmented by 10- 100-fold. Although fixed cells, unlike live do...