A5103141052- Ovarian cancer diagnosis and treatment
- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
- Endometrial and Cervical Cancer Treatments
- Catalytic Cross-Coupling Reactions
- Immune Response and Inflammation
- Catalytic C–H Functionalization Methods
- Reproductive System and Pregnancy
- Bone health and osteoporosis research
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Retinoids in leukemia and cellular processes
- Neuroendocrine Tumor Research Advances
- Uterine Myomas and Treatments
- Organoboron and organosilicon chemistry
- Chemical Synthesis and Analysis
- Synthesis and Characterization of Pyrroles
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Lipids, and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hypothalamic control of reproductive hormones
- Gestational Trophoblastic Disease Studies
- Diabetes Treatment and Management
Osaka Police Hospital
2010-2022
Toray Industries, Inc. (Japan)
2018
Sumitomo Dainippon Pharma (Japan)
2010-2011
Osaka University
1996-2010
Kansai Rosai Hospital
2006-2010
Cancer Genetics (United States)
2010
Osaka Medical and Pharmaceutical University
2010
Fox Chase Cancer Center
2010
NTN (Japan)
2010
Wakayama Medical University
2008
Single binding sites for transcription factors NF-IL6 and NF-kappa B are present in the promoter of interleukin (IL) 6 gene. Previous studies internally deleted mutants demonstrated that these two important transcriptional regulation this In report, we describe synergistic activation IL-6 by B. Cotransfection with p65 subunit resulted strong an promoter-reporter construct. Both were required activation. Similar was observed IL-8 promoter, which also contains both sites. Furthermore, directly...
Although estrogen is known to activate endothelial nitric oxide synthase (eNOS) in the vascular endothelium, molecular mechanism responsible for this effect remains be elucidated. In studies of both human umbilical vein cells (HUVECs) and simian virus 40-transformed rat lung (TRLECs), 17β-estradiol (E2), but not 17α-E2, caused acute activation eNOS that was unaffected by actinomycin D specifically blocked pure receptor antagonist ICI-182,780. Treatment TRLECs HUVECs with 17β-E2 stimulated...
The acute-phase reaction is accompanied by an increase in a variety of serum proteins, named proteins. synthesis these proteins synergistically controlled glucocorticoids and inflammatory cytokines such as interleukin-1 (IL-1), IL-6, tumor necrosis factor alpha. Recently, we have cloned nuclear factor-IL-6 (NF-IL6), transcription that activates the IL-6 gene, demonstrated its involvement expression acute-phase-protein genes. We report here analysis molecular mechanisms which glucocorticoid...
Whether or not inhibition of NFκB increases the efficacy cisplatin in vitro and vivo ovarian cancer models was investigated. We compared basal levels phosphorylation IκBα activity between cisplatin-sensitive A2780 cells cisplatin-resistant Caov-3 cells. The were significantly higher than those Cisplatin caused a more marked decrease Thus, high activation less by seem to reduce sensitivity cisplatin. Inhibition either treatment with inhibitor (BAY 11-7085) specific nuclear translocation...
Estrogen exerts a variety of effects not only on female reproductive organs but also nonreproductive organs, including adipose tissue. inhibits obesity triggered by ovariectomy in rodents. We studied the mechanism underlying this estrogen-dependent inhibition obesity. markedly decreased amounts fat accumulation and lipoprotein lipase (LPL) mRNA as well triglyceride genetically manipulated 3T3-L1 adipocytes stably expressing estrogen receptor (ER). A pLPL(1980)-CAT construct, along with an ER...
We studied the roles of phosphatidylinositol 3-kinase (PI-3K)-Akt-BAD cascade, ERK-BAD and Akt-Raf-1 cascade in paclitaxel-resistant SW626 human ovarian cancer cell line, which lacks functional p53. Treatment cells with paclitaxel activates Akt ERK different time frames. Interference either by treatment PI-3K inhibitor (wortmannin or LY294002) exogenous expression a dominant negative caused decreased viability following paclitaxel. an MEK inhibitor, PD98059, also stimulated phosphorylation...
The preceding paper (Cha, H. H., Cram, E. J., Wang, C., Huang, A. Kasler, G., and Firestone, G. L. (1998) J. Biol. Chem. 273, 0000-0000(478563) defined a glucocorticoid responsive region within teh promoter of the p21 CDK inhibitor gene that contains putative DNA-binding site for transcription factor CCAAT/ enhancer binding protein-alpha (C/EBP alpha). Wild type rat BDS1 hepatoma cells as well as4 cells, which express antisense sequences to C/EBP alpha ablate its protein production, were...
Aromatic CH silylation of arenes by 1,2-di-tert-butyl-1,1,2,2-tetrafluorodisilane is efficiently catalyzed iridium(I) complexes generated from [{Ir(OMe)(cod)}2] (cod=cyclooctadiene) and 4,4′-di-tert-butyl-2,2′-bipyridine (dtbpy; see scheme) provides the corresponding aryl fluorosilanes in excellent yields.
Abstract We investigated whether inhibition of nuclear factor-κB (NFκB) increases the efficacy paclitaxel in vitro and vivo ovarian cancer models. Treatment paclitaxel-sensitive Caov-3 cells with transiently activated phosphorylation Akt, IκB kinase (IKK), inhibitor NFκB (IκBα). Paclitaxel also caused a transient increase activity, followed by decrease activity. show an association between Akt IKK that induced is blocked treatment phosphatidylinositol 3-kinase (wortmannin or LY294002)....
The acute-phase reaction is accompanied by an increase in a variety of serum proteins, named proteins. synthesis these proteins synergistically controlled glucocorticoids and inflammatory cytokines such as interleukin-1 (IL-1), IL-6, tumor necrosis factor alpha. Recently, we have cloned nuclear factor-IL-6 (NF-IL6), transcription that activates the IL-6 gene, demonstrated its involvement expression acute-phase-protein genes. We report here analysis molecular mechanisms which glucocorticoid...
Abstract This study examines the role of vascular endothelial growth factor (VEGF) as a therapeutic target in clear cell carcinoma (CCC) ovary, which has been regarded chemoresistant histologic subtype. Immunohistochemical analysis using tissue microarrays 98 primary ovarian cancers revealed that VEGF was strongly expressed both early-stage and advanced-stage CCC ovary. In CCCs, patients who had tumors with high levels significantly shorter survival than those low VEGF. vitro experiments...
Cytokines and steroid hormones use different sets of signal transduction pathways, which seem to be unrelated. Interleukin-6 (IL-6) uses JAK tyrosine kinase STAT (signal transducer activator transcription) transcription factor. Glucocorticoid binds glucocorticoid receptor (GR), is a member the superfamily. We have studied crosstalk between IL-6-JAK-STAT glucocorticoid-nuclear pathways. IL-6 synergistically activated response element on rat alpha2-macroglobulin promoter (APRE)-driven...
The aromatic C-H silylation of five-membered heteroarenes with 1,2-di-tert-butyl-1,1,2,2-tetrafluorodisilane regioselectively proceeded at 120 degrees C in octane the presence a catalytic amount iridium(I) complexes generated from 1/2[Ir(OMe)(COD)]2 and 2-tert-butyl-1,10-phenanthroline.
The aromatic CH silylation of arenes (10 equiv) with 1,2-di-sec-butyl-1,1,2,2-tetrafluorodisilane was carried out in octane at 120 °C the presence a catalytic amount iridium(I) complexes (3.0 mol %) generated from 1/2[Ir(OMe)(COD)]2 and 2,9-diisopropyl-1,10-phenanthroline. reactions many resulted formation corresponding arylfluorosilanes high yields excellent regioselectivities.
Patients with Turner syndrome (TS) almost develop osteoporosis, resulting from chromosomal deficiency and estrogen by gonadal dysgenesis. The aim of this study was to assess bone mineral density (BMD) during continuous therapy in young TS patients measuring lumbar spine BMD 67 using dual-energy X-ray absorptiometry. Twenty-seven who were treated adult-doses prior the first evaluation, exhibited a significantly higher initial than 30 low-dose 10 without (0.808 g/cm², 0.714 0.664...
Raloxifene is a tissue-selective estrogen receptor modulator. The effect of on cardiovascular disease mainly dependent direct actions the vascular wall involving activation endothelial nitric oxide synthase (eNOS) via Akt and extracellular signal-regulated protein kinase (ERK) cascades. Although raloxifene also known to activate eNOS in endothelium, molecular mechanism responsible for this remains be elucidated. In studies both human umbilical vein cells simian virus 40-transformed rat lung...