A5038943819- PARP inhibition in cancer therapy
- Telomeres, Telomerase, and Senescence
- Biosimilars and Bioanalytical Methods
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- DNA Repair Mechanisms
- Viral-associated cancers and disorders
- Health Systems, Economic Evaluations, Quality of Life
- Banana Cultivation and Research
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
- Herpesvirus Infections and Treatments
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Ion channel regulation and function
- Mycobacterium research and diagnosis
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Identification and Quantification in Food
- Genomics and Chromatin Dynamics
National Institutes of Health
2020-2024
National Cancer Institute
2020-2024
GlaxoSmithKline (United States)
2024
Shandong University
2024
China Medical University
2019-2023
Center for Cancer Research
2020-2023
Yuncheng University
2023
Chinese University of Hong Kong, Shenzhen
2023
Chinese Academy of Tropical Agricultural Sciences
2013-2021
Jiangsu Cancer Hospital
2020
It is unclear when the pathologic features of asthma first appear. We hypothesized that eosinophilic airway inflammation and epithelial reticular basement membrane (RBM) thickening, absent in wheezy infants, would be present preschool children with severe, recurrent wheeze.To compare RBM thickness endobronchial biopsies (EBs) from age-matched control subjects.EBs were obtained (aged 3 mo to 5 yr), undergoing a clinically indicated fiberoptic bronchoscopy. Subjects bronchoscopy investigate...
Exosomes (Exo) hold great promise as endogenous nanocarriers that can deliver biological information between cells. However, Exo are limited in terms of their abilities to target specific recipient cell types. We developed a strategy isolate exhibiting increased binding integrin αvβ3. Binding occurred through modified version disintegrin and metalloproteinase 15 (A15) expressed on exosomal membranes (A15-Exo), which facilitated co-delivery therapeutic quantities doxorubicin (Dox)...
Cranial bone defect remains a major challenge to craniofacial surgeons because of limited availability autologous graft repair the defects and donor site secondary tissue harvesting. In contrast, tissue-engineering technique can generate large using small amount cells therefore avoid these problems. Bone Marrow Stromal Cells (MSCs) have potential multi-lineage (including osteogenic) differentiation. The objective this study was investigate MSCs cranial by approach. Autologous were isolated...
Abstract Crosstalk between hematopoietic stem cells (HSCs) and the comprising niche is critical for maintaining cell activities. Yet little evidence supports concept that HSCs regulate development of niche. Here, ability to directly endosteal was examined. Marrow isolated 48 hours after “stressing” mice with a single acute bleed or from control nonstressed animals. “Stressed” “nonstressed” were cocultured bone marrow stromal map mesenchymal fate. The data suggest are able guide...
To enhance the understanding of differentiation patterns and bone formation capacity hESCs, we determined (1) temporal pattern osteoblastic human embryonic stem cell-derived mesenchymal cells (hESC-MSCs), (2) influence a three-dimensional matrix on osteogenic hESC-MSCs in long-term culture, (3) bone-forming osteoblast-like derived from calvarial defects. Incubation medium induced into mature osteoblasts similar chronological to marrow stromal primary osteoblasts. Osteogenic was enhanced by...
Macrophages dictate both initiation and resolution of inflammation. During acute inflammation classically activated macrophages (M1) predominate, during the phase alternative (M2) are dominant. The molecular mechanisms involved in macrophage polarization understudied. MicroRNAs differentially expressed M1 M2 that influence polarization. We identified a role miR-21 polarization, found cross-talk between lipid mediator prostaglandin E2 (PGE2) is determining factor inhibition impairs expression...
Significance Telomeres protect the ends of eukaryotic chromosomes. shorten with age and serve as a biological clock that limits cell proliferation. Excessive telomere shortening accelerates aging, but elongation may facilitate cancer. We found inherited mutations in regulator helicase 1 (RTEL1), which cause Hoyeraal–Hreidarsson syndrome, fatal disease characterized by accelerated shortening, immunodeficiency, developmental defects. Introducing normal RTEL1 gene into affected cells prevented...
Abstract Sugars Will Eventually be Exported Transporters (SWEET) are a novel type of sugar transporter that plays crucial roles in multiple biological processes. From banana, for the first time, 25 SWEET genes which could classified into four subfamilies were identified. Majority MaSWEETs each subfamily shared similar gene structures and conserved motifs. Comprehensive transcriptomic analysis two banana genotypes revealed differential expression patterns different tissues, at various stages...
Significance Loss of telomere repeats leads to cellular senescence and the secretion inflammatory cytokines. How dysfunction is linked this phenotype its role in aging cancer not yet understood. We show here that noncoding RNA transcripts [telomeric repeat-containing (TERRA)] are secreted into extracellular environment exosome vesicle fractions. This cell-free TERRA (cfTERRA) shorter more stable than intracellular TERRA, associated with histone proteins, can induce cytokines responsive...
Osteoporosis might be due to defects in mesenchymal stem cells (MSCs) that lead reduced proliferation and osteoblast differentiation. We hypothesized transplantation of MSCs into sites at risk for developing osteoporotic bone could improve structure biomechanics. The aim this study was establish an osteoporosis rabbit model by ovariectomy (OVX), characterize the autologous from OVX rabbits, transplant rabbits. harvested marrow normal rabbits were culture expanded differentiated osteogenic...
A prospective in vivo assay was used to identify cells with potential for multiple lineage differentiation. With this assay, it first determined that the 5-fluorouracil resistant capable of osseous tissue formation also migrated toward stromal derived factor-1 (SDF-1) vitro. In parallel, an isolation method based on fluorescence-activated cell sorting employed a very small embryonic-like Lin−Sca-1+CD45− as few 500 forming bone-like structures vivo. Differential marrow fractionation studies...
Telomere repeat DNA forms a nucleo-protein structure that can obstruct chromosomal replication, especially under conditions of replication stress. Transcription telomere repeats initiate at subtelomeric CTCF-binding sites to generate repeat-encoding RNA (TERRA), but the role transcription, CTCF, and TERRA in is not known. Here, we have used CRISPR/Cas9 gene editing mutate putative start site transcripts for class subtelomeres. Under stress, telomeres lacking CTCF-driven exhibit...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Telomeres play critical roles in the maintenance of genome integrity and control cellular senescence. Most eukaryotic telomeres can be transcribed to generate a telomeric repeat-containing RNA (TERRA) that persists as heterogeneous nuclear developmentally regulated. However, precise function regulation TERRA normal cancer cell development remains poorly understood. Here, we show accumulates highly proliferating cells, forms large foci, which are distinct from previously characterized markers...
Abstract MicroRNAs are known to control TLR activation in phagocytes. We have shown that leukotriene (LT) B4 (LTB4) positively regulates macrophage MyD88 expression by decreasing suppressor of cytokine signaling-1 (SOCS-1) mRNA stability. In this study, we investigated the possibility LTB4 involves generation microRNAs. Our data show LTB4, via its receptor B 1 (BLT1) and Gαi signaling, increased inflammatory microRNAs, including miR-155, miR-146b, miR-125b. LTB4-mediated miR-155 was...
Clostridium perfringens α-toxin is one of the major virulence factors during C. infection, causing hemolysis erythrocytes in various species. Here, genetically engineered Lactobacillus casei (pPG-α/L. 393) constitutively expressing toxoid was generated and its immunogenicity mice for induction protective immunity against evaluated via oral immunization. The α-toxoid expressed by pPG-α/L. 393 without a specific inducer, as confirmed western blotting, laser confocal microscopy, flow cytometry....
Proteins encoded by MADS-box genes are important transcription factors involved in the regulation of flowering plant growth and development. Currently, no systematic information exists regarding family tropical fruit banana. Ninety-six were identified from banana (Pahang) A genome. Phylogenetic analysis indicated that Musa acuminata MCM1-AGAMOUS- DEFICIENS-SRF (MaMADS) could be divided into MIKCc, MIKC*, Mα/β Mγ groups. MIKCc further 11 subfamilies, which was supported conserved motif gene...