A5012403026- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Ion Channels and Receptors
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- Lymphoma Diagnosis and Treatment
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Heat shock proteins research
- RNA Research and Splicing
- Calcium signaling and nucleotide metabolism
- Bioactive Compounds and Antitumor Agents
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
- Mast cells and histamine
University of Göttingen
2011-2025
Universitätsmedizin Göttingen
2015-2023
University Medical Center
2016
Bielefeld University
2002-2004
Universidad Autónoma de Madrid
2002
CEA Le Ripault
2002
Consejo Superior de Investigaciones Científicas
2002
Weizmann Institute of Science
1999
Kiel University
1997
Regulated intramembrane proteolysis is a central cellular process involved in signal transduction and membrane protein turnover. The presenilin homologue signal-peptide-peptidase-like 2a (SPPL2a) has been implicated the cleavage of type 2 transmembrane proteins. We show that invariant chain (li, CD74) major histocompatability class II complex (MHCII) undergoes mediated by SPPL2a. B lymphocytes SPPL2a−/− mice accumulate an N-terminal fragment (NTF) CD74, which severely impairs traffic within...
The hallmarks of dry skin (xerosis) are scaliness and loss elasticity. Decreased hydration a disturbed lipid content the stratum corneum also well-known features. frequency increases with ageing. aim this study was to examine if these known features related changes in epidermal proliferation differentiation. In addition, age-related normal were examined: 62 volunteers divided by clinical grading biophysical measurements into groups young/normal, young/dry, aged/normal aged/dry skin. Biopsy...
Research Article19 October 2015Open Access The MICA-129 dimorphism affects NKG2D signaling and outcome of hematopoietic stem cell transplantation Antje Isernhagen Institute Cellular Molecular Immunology, University Medical Center Göttingen, Germany Search for more papers by this author Dörthe Malzahn Genetic Epidemiology, Elena Viktorova Leslie Elsner Sebastian Monecke Frederike von Bonin Department Hematology Oncology, Markus Kilisch Biology, Janne Marieke Wermuth Neele Walther Yesilda...
Significance B-cell receptor (BCR) signaling promotes the survival of malignant B cells, such as Burkitt’s lymphoma (BL) and activated B-cell–like subtype diffuse large (ABC-DLBCL). In contrast to ABC-DLBCL, which depends on chronic activation BCR, BL cells rely tonic BCR that is antigen-independent. Elucidation systematic comparison led identification novel effectors, including ACTN4 ARFGEF2, were identified regulators BL-cell survival. Beyond its relevance understanding pathogenesis...
The aminopeptidase DPP9 removes dipeptides from N-termini of substrates having a proline or alanine in second position. Although linked to several pathways including cell survival and metabolism, the molecular mechanisms underlying these outcomes are poorly understood. We identified novel interaction with Filamin A, which recruits Syk, central kinase B-cell signalling. Syk signalling can be terminated by degradation, requiring ubiquitin E3 ligase Cbl. show that cleaves produce neo N-terminus...
Peptides and proteins, that have the tendency to form aggregates, are often discussed in context of Alz-heimer’s, Huntington’s, or Parkinson’s disease. However, studying aggregation processes is inherently challenging due diversity aggregate size geometry lack control over process space time. Here, we present a small, synthetic peptide, for which can be controlled reversibly with light within seconds. Specifically, by incorporating photoswitchable unnatural amino acids into sequence...
Abstract Several lines of evidence have indicated that changes in the structure neuronal cytoskeleton provide support for dramatic morphological occur during differentiation. It has been proposed microtubule‐associated proteins can contribute to development this phenomenon by controlling dynamic properties microtubules. In report we characterized effect combined suppression MAP1B and tau, MAP2 on polarization cultured hippocampal cells grown a laminin‐containing substrate. We taken advantage...
Abstract Antibody-mediated immune responses rely on antigen recognition by the B cell receptor (BCR) and proper engagement of its intracellular signal effector proteins. Src homology (SH) 2 domain-containing leukocyte protein 65 kDa (SLP65) is key scaffold mediating BCR signaling. In resting cells, SLP65 colocalizes with Cbl-interacting 85 (CIN85) in cytoplasmic granules whose formation not fully understood. Here we show that effective activation requires tripartite phase separation SLP65,...
Spleen tyrosine kinase Syk provides critical transducer functions for a number of immune cell receptors and has been implicated in the generation several forms leukemias. Catalytic activity ability to interact with other signaling elements depend on phosphorylation status Syk. We have now identified quantified full spectrum phosphoacceptor sites human as well interactome resting activated B cells by high-resolution mass spectrometry. While majority inducible phosphorylations occurred...
We reported recently that the presenilin homologue signal peptide peptidase-like 2a (SPPL2a) is essential for B cell development by cleaving N-terminal fragment (NTF) of invariant chain (li, CD74). Based on this, we suggested pharmacological modulation SPPL2a may represent a novel approach to deplete cells in autoimmune disorders. With regard overlapping substrate spectra and its close homologue, SPPL2b, investigated role SPPL2b CD74 NTF proteolysis impact dendritic homeostasis. In...
Transient receptor potential proteins (TRP) are supposed to participate in the formation of store‐operated Ca 2+ influx channels by co‐assembly. However, little is known which domains facilitate interaction subunits. Contribution N‐terminal coiled‐coil domain and ankyrin‐like repeats putative pore region mouse TRP1β (mTRP1β) variant functional cation were analyzed following overexpression HEK293 (human embryonic kidney) cells. MTRP1β expressing cells exhibited enhanced whole‐cell membrane...
Optimal B cell activation requires the recruitment of a vesicle-associated adaptor protein to receptor complex.
The SH2 domain-containing inositol 5'-phosphatase (SHIP) plays a key role in preventing autoimmune phenomena by limiting antigen-mediated B cell activation. SHIP function is thought to require the dual engagement of BCR and negative regulatory coreceptors as only latter appear capable recruiting from cytosol plasma membrane virtue phosphorylated immunoreceptor tyrosine-based inhibitory motifs. Here, we demonstrate coreceptor-independent recruitment cells. In absence coreceptor ligation,...