A5049558604- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Plant nutrient uptake and metabolism
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Genetics, Bioinformatics, and Biomedical Research
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Virus Research Studies
- Cancer-related gene regulation
Oak Ridge National Laboratory
2025
University of California, Los Angeles
2018-2024
Enzymatic activity of histones Eukaryotic serve as structural elements to package DNA. However, they contain a copper-binding site for which the biological relevance is unknown. Copper homeostasis critical several fundamental eukaryotic processes, including mitochondrial respiration. Attar et al. hypothesized that may play role in cellular copper utilization (see Perspective by Rudolph and Luger). Using multifaceted approach ranging from vitro biochemistry vivo genetic molecular analyses,...
Methyl readers that repress transcription DNA methylation is a conserved epigenetic mark required for gene silencing in many different organisms. However, how the methyl able to silence genes still largely unknown. Ichino et al. discovered two Arabidopsis proteins named MBD5 and MBD6 are recruited by direct recognition of methylation. These recruit class C J-domain protein SILENZIO chromatin methylated transposons. likely acts through its interaction with heat shock chaperone proteins....
DNA methylation is an essential component of transposable element (TE) silencing, yet the mechanism by which causes transcriptional repression remains poorly understood
Summary RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) external (environmental) stimuli, paving the way for advances biotechnology engineer crops with improved resilience, quality productivity. In general, traditional methods analysis plants require destructive, labour‐intensive time‐consuming assays. To...
DNA methylation mediates silencing of transposable elements and genes in part via recruitment the Arabidopsis MBD5/6 complex, which contains methyl-CpG binding domain (MBD) proteins MBD5 MBD6, J-domain containing protein SILENZIO (SLN). Here, we characterize two additional complex members: α-crystalline (ACD) ACD15 ACD21. We show that they are necessary for gene silencing, bridge SLN to promote higher-order multimerization complexes within heterochromatin. These also highly dynamic, with...
Proteins are localized and concentrated at cellular genomic locations for specific efficient functions. Efforts to understand protein accumulation in eukaryotic organisms have primarily focused on multivalent interactions between intrinsically disordered regions (IDRs) as mediators of condensation. We previously showed that α-crystalline domain (ACD) proteins 15 (ACD15) 21 (ACD21) were required multimerization the gene-silencing methyl-CpG-binding 6 (MBD6) chromocenters
Detection of gene expression in plants is critical for understanding the molecular basis complex plant biosystems and responses to environmental stresses. Here, we report development a split ribozyme-based biosensor that enables vivo visualization plants. We demonstrated utility this transient experiments (i.e., leaf infiltration Nicotiana benthamiana) detect RNAs derived from transgenes tobacco rattle virus, respectively. Furthermore, successfully engineered Arabidopsis thaliana endogenous...
DNA methylation mediates silencing of transposable elements and genes in part via recruitment the Arabidopsis MBD5/6 complex, which contains methyl-CpG-binding domain (MBD) proteins MBD5 MBD6, J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline ACD15 ACD21. We show that they are necessary for gene silencing, bridge SLN to promote higher order multimerization complexes within heterochromatin. These also highly dynamic, with mobility...
Abstract Ancestral histones were present in organisms with small genomes, no nucleus, and little evidence for epigenetic regulation, suggesting may have additional older functions. We report that the histone H3-H4 tetramer is an enzyme catalyzes reduction of Cu 2+ to 1+ when assembled vitro from recombinant histones. Mutations residues putative active site at interface apposing H3 proteins alter enzymatic activity cellular processes such as Sod1 function or mitochondrial respiration depend...
Abstract DNA methylation is an essential component of transposable element (TE) silencing, yet the mechanism by which causes transcriptional repression remains poorly understood 1–5 . Here we study Arabidopsis thaliana Methyl-CpG Binding Domain (MBD) proteins MBD1, MBD2, and MBD4, show that MBD2 acts as a repressor during male gametogenesis. bound chromatin regions containing high levels CG methylation, was capable silencing FWA gene when tethered to its promoter. loss caused TE activation...