A5057558341- Toxoplasma gondii Research Studies
- Biotin and Related Studies
- Click Chemistry and Applications
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Plant Molecular Biology Research
- Reproductive System and Pregnancy
- Microbial Fuel Cells and Bioremediation
- Mosquito-borne diseases and control
- Reproductive tract infections research
- Ubiquitin and proteasome pathways
- Plant nutrient uptake and metabolism
- Genomics and Chromatin Dynamics
- Autophagy in Disease and Therapy
- Complement system in diseases
- Wastewater Treatment and Nitrogen Removal
- Tryptophan and brain disorders
- Preterm Birth and Chorioamnionitis
- Virus-based gene therapy research
- Long-Term Effects of COVID-19
- Smoking Behavior and Cessation
- Melanoma and MAPK Pathways
- Analytical Chemistry and Chromatography
University of California, Los Angeles
2019-2025
Thermo Fisher Scientific (United States)
2023
APLA Health
2022
The
Trichomonas vaginalis, the etiologic agent of most common non-viral sexually transmitted infection worldwide. With an estimated annual prevalence 276 million new cases, mixed infections with different parasite strains are expected. Although it is known that parasites interact their host to enhance own survival and transmission, evidence call into question extent which unicellular communicate each other. Here, we demonstrated T. vaginalis can through formation cytoneme-like membranous cell...
Toxoplasma gondii resides in its intracellular niche by employing a series of specialized secretory organelles that play roles invasion, host cell manipulation, and parasite replication. Rab GTPases are major regulators the parasite’s traffic function as nucleotide-dependent molecular switches to control vesicle trafficking. While many proteins have been characterized T . , precisely how these Rabs regulated remains poorly understood. To better understand traffic, we investigated entire...
Apicomplexan parasites use a specialized cilium structure called the apical complex to organize their secretory organelles and invasion machinery. The is integrally associated with both parasite plasma membrane an intermediate filament cytoskeleton inner-membrane (IMC). While essential parasitic lifestyle, little known about regulation of biogenesis. Here, we identify AC9 (apical cap protein 9), largely intrinsically disordered component Toxoplasma gondii IMC, as for development, therefore...
Abstract The Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants animals, C. elegans MORC-1 has been shown to topologically entrap condense DNA. In Arabidopsis thaliana , mutation MORCs reactivate silent methylated genes transposons decondense heterochromatic chromocenters, despite only minor changes the maintenance DNA methylation. Here we provide first evidence localizing MORC proteins specific regions...
ABSTRACT Obligate intracellular malaria parasites dramatically remodel their erythrocyte host through effector protein export to create a niche for survival. Most exported proteins contain pentameric P lasmodium ex port el ement (PEXEL)/host-targeting motif that is cleaved in the parasite ER by aspartic protease Plasmepsin V (PMV). This processing event exposes mature N terminus required translocation into cell and not known occur non-exported proteins. Here, we report parasitophorous...
DNA methylation plays crucial roles in gene regulation and transposon silencing. In plants, the maintenance of is controlled by several self-reinforcing loops involving histone non-coding RNAs. However, how initially patterned at specific loci unknown. Here, we describe four Arabidopsis REM transcription factors, VDD, VAL, REM12 REM13, that recognize sequence regions, together with protein GENETICS DETERMINES EPIGENETICS1 (GDE1), recruit RNA polymerase IV complexes to generate 24-nucleotide...
ABSTRACT Adipose tissue has varying distributions and metabolic properties between the sexes. Inherent sex‐specific differences in adipocytes may heighten risk of disease males. Analysis adipocyte proteome can potentially provide important insight. To enable cell‐type specific proteomic profiling vivo, we genetically engineered a mouse line for production promiscuous biotin ligase (BirA*G3) facilitating rapid isolation biotinylated proteomes. Adipocyte‐specific activation cytoplasmic BirA*G3...
Organ functions are highly specialized and interdependent. Secreted factors regulate organ development mediate homeostasis through serum trafficking inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report BirA*G3 mouse strain that CRE-dependent promiscuous biotinylation endoplasmic reticulum. When broadly activated throughout mouse, widespread was observed secretory pathway. Streptavidin...
Motility of pathogenic protozoa depends on flagella (synonymous with cilia) axonemes containing nine doublet microtubules (DMTs) and two singlet microtubules. Microtubule inner proteins (MIPs) within DMTs influence axoneme stability motility provide lineage-specific adaptations, but individual MIP functions assembly mechanisms are mostly unknown. Here, we show in the sleeping sickness parasite Trypanosoma brucei, that FAP106, a conserved at DMT junction, is required for trypanosome as...
Abstract Toxoplasma gondii resides in its intracellular niche by employing a series of specialized secretory organelles that play roles invasion, host-cell manipulation and parasite replication. Rab GTPases are major regulators the parasite’s traffic function as nucleotide dependent molecular switches to control vesicle trafficking. While many proteins have been characterized T. , precisely how these Rabs regulated remains poorly understood. To better understand traffic, we investigated...
A hybrid approach combining water-splitting electrochemistry and H 2 -oxidizing, CO -fixing microorganisms offers a viable solution for producing value-added chemicals from sunlight, water, air. The classic wisdom without thorough examination to date assumes that the in such -mediated process is innocent of altering microbial behavior. Here, we report unexpected metabolic rewiring induced by -oxidizing acetogenic bacterium Sporomusa ovata challenges view. We found planktonic S. more...
DNA methylation mediates silencing of transposable elements and genes in part via recruitment the Arabidopsis MBD5/6 complex, which contains methyl-CpG binding domain (MBD) proteins MBD5 MBD6, J-domain containing protein SILENZIO (SLN). Here, we characterize two additional complex members: α-crystalline (ACD) ACD15 ACD21. We show that they are necessary for gene silencing, bridge SLN to promote higher-order multimerization complexes within heterochromatin. These also highly dynamic, with...
G-protein-coupled receptors (GPCRs) initiate intracellular signaling events through heterotrimeric G-protein α-subunits (Gα) and the βγ-subunit dimer (Gβγ). In this study, we utilized mass spectrometry to identify novel regulators of Gβγ in human cells. This prompted our characterization KCTD2 KCTD5, two related potassium channel tetramerization domain (KCTD) proteins that specifically recognize Gβγ. We demonstrated these KCTD are substrate adaptors for a multisubunit CUL3–RING ubiquitin...
The inner membrane complex (IMC) of Toxoplasma gondii is essential for all phases the parasite's life cycle. One its most critical roles to act as a scaffold assembly daughter buds during replication by endodyogeny. While many IMC proteins have been identified, are recruited after bud initiation and not parasite fitness. Here, we report identification IMC43, novel protein that at earliest stages initiation. Using an auxin-inducible degron system show depletion IMC43 results in aberrant...
The cytosolic iron–sulfur (Fe-S) cluster assembly (CIA) pathway delivers Fe-S clusters to nuclear and proteins involved in essential cellular functions. Although the delivery process is regulated by availability of iron oxygen, it remains unclear how CIA components orchestrate transfer under varying environments. Here, we utilized a targeted proteomics assay for monitoring factors substrates characterize machinery. We find that nucleotide-binding protein 1 (NUBP1/NBP35), component 3...
Deep proteome coverage in bottom-up proteomics requires peptide-level fractionation to simplify the complex peptide mixture before analysis by tandem mass spectrometry. By decreasing number of coeluting precursor ions, effectively reduces complexity sample leading higher and reduced bias toward high-abundance precursors that are preferentially identified data-dependent acquisition strategies. To achieve this goal, we report a bead-based off-line method termed CIF or carboxylate-modified...
The cytoskeleton of Toxoplasma gondii is composed the inner membrane complex (IMC) and an array underlying microtubules that provide support at periphery parasite. Specific subregions IMC carry out distinct roles in replication, motility, host cell invasion. Building on our previous vivo biotinylation (BioID) experiments IMC, we identified here a novel protein localizes to discrete puncta are embedded parasite's along sutures. Gene knockout analysis showed loss results defects cytoskeletal...
possesses a highly polarized secretory pathway that contains both broadly conserved eukaryotic organelles and unique apicomplexan which play essential roles in the parasite's lytic cycle. As other eukaryotes,
Biological-inorganic hybrid systems are a growing class of technologies that combine microorganisms with materials for many purposes including chemical synthesis, environmental remediation, and energy generation. Recently, have been developed toward the sustainable generation value-added chemicals from plentiful potentially renewable resources electricity, water, air. These typically consider as catalysts essentially perform only reaction interest, however other metabolic activity may...
ABSTRACT Toxoplasma gondii possesses a highly polarized secretory pathway that contains both broadly conserved eukaryotic organelles and unique apicomplexan organelles, which play essential roles in the parasite’s lytic cycle. As other eukaryotes, T. Golgi apparatus sorts modifies proteins prior to their distribution downstream organelles. Many of typical trafficking factors found involved these processes are missing from genomes, suggesting parasites have evolved fill roles. Here, we...