A5051455214- Trypanosoma species research and implications
- Ionosphere and magnetosphere dynamics
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Atmospheric chemistry and aerosols
- Magnetic confinement fusion research
- Lysosomal Storage Disorders Research
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Calcium signaling and nucleotide metabolism
- Ubiquitin and proteasome pathways
- Biochemical and Molecular Research
- Advanced Fluorescence Microscopy Techniques
- Parasites and Host Interactions
- Plasma Diagnostics and Applications
- Particle accelerators and beam dynamics
- Atmospheric aerosols and clouds
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Adenosine and Purinergic Signaling
- Plant Parasitism and Resistance
- Digital Holography and Microscopy
- Endoplasmic Reticulum Stress and Disease
- Photoacoustic and Ultrasonic Imaging
- Vector-borne infectious diseases
University of California, Los Angeles
2011-2025
University of Washington
2006-2010
Seattle University
2010
Earth and Space Research
2009
The flagellum of African trypanosomes is an essential and multifunctional organelle that functions in motility, cell morphogenesis, host-parasite interaction. Previous studies the trypanosome have been limited by inability to purify flagella without first removing flagellar membrane. This limitation particularly relevant context studying signaling, as signaling requires surface-exposed proteins membrane soluble matrix. Here we employ a combination genetic mechanical approaches intact from...
Increasing evidence indicates that the Trypanosoma brucei flagellum (synonymous with cilium) plays important roles in host-parasite interactions. Several studies have identified virulence factors and signaling proteins flagellar membrane of bloodstream-stage T. brucei, but less is known about procyclic, insect-stage parasites. Here we report on identification several receptor-type adenylate cyclases (ACs) are specifically upregulated procyclic Identification insect stage-specific ACs novel,...
Parasitic infections constitute a major global public health issue. Existing screening methods that are based on manual microscopic examination often struggle to provide sufficient volumetric throughput and sensitivity facilitate early diagnosis. Here, we demonstrate motility-based label-free computational imaging platform rapidly detect motile parasites in optically dense bodily fluids by utilizing the locomotion of as specific biomarker endogenous contrast mechanism. Based this principle,...
African trypanosomes, Trypanosoma brucei spp., are lethal pathogens that cause substantial human suffering and limit economic development in some of the world's most impoverished regions. The name ("auger cell") derives from parasite's distinctive motility, which is driven by a single flagellum. However, despite decades study, requirement for trypanosome motility mammalian host infection has not been established. LC1 conserved dynein subunit required flagellar motility. Prior studies with...
African trypanosomes are devastating human and animal pathogens transmitted by tsetse flies between mammalian hosts. The trypanosome surface forms a critical host interface that is essential for sensing adapting to diverse environments. However, protein composition diversity remain largely unknown. Here, we use labeling, affinity purification, proteomic analyses describe cell proteomes from insect-stage bloodstream-stage Trypanosoma brucei. contain most previously characterized proteins. We...
The flagellum of Trypanosoma brucei drives the parasite’s characteristic screw-like motion and is essential for its replication, transmission, pathogenesis. However, molecular details this process remain unclear. Here, we present high-resolution (up to 2.8 angstrom) cryo–electron microscopy structures T. flagellar doublet microtubules (DMTs). Integrated modeling identified 154 different axonemal proteins inside outside DMT and, together with genetic proteomic interrogation, revealed...
Significance We combine genetics, biochemistry, cell biology, and proteomics to define Bardet–Biedl Syndrome complex (BBSome) composition, location, function in the deadly parasite Trypanosoma brucei . BBSome mutants have reduced infectivity mice, quantitative identified surface proteome changes that may underlie virulence. To our knowledge, this work presents first comprehensive study of any microbial pathogen. T. is also among earliest organisms diverged from other eukaryotes, showing deep...
Motility of pathogenic protozoa depends on flagella (synonymous with cilia) axonemes containing nine doublet microtubules (DMTs) and two singlet microtubules. Microtubule inner proteins (MIPs) within DMTs influence axoneme stability motility provide lineage-specific adaptations, but individual MIP functions assembly mechanisms are mostly unknown. Here, we show in the sleeping sickness parasite Trypanosoma brucei, that FAP106, a conserved at DMT junction, is required for trypanosome as...
Abstract. MASS (Mesospheric Aerosol Sampling Spectrometer) is a multichannel mass spectrometer for charged aerosol particles, which was flown from the Andøya Rocket Range, Norway, through NLC and PMSE on 3 August 2007 6 2007. The eight-channel analyzers provided first time simultaneous measurements of charge density residing particles in four ranges, corresponding to ice with radii <0.5 nm (including ions), 0.5–1 nm, 1–2 >3 (approximately). Positive negative were recorded separate...
Sleeping sickness is a neglected tropical disease caused by the protozoan parasite Trypanosoma brucei . The disrupts sleep-wake cycle, leading to coma and death if left untreated. T. motility, transmission, virulence depend on its flagellum (cilium), which consists of several different specialized subdomains.
Yeast replicative aging is a process resembling in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene studied via microarrays quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant messenger RNA (mRNA) genes at 12 18-20 generations. Our findings support...
The Saccharomyces cerevisiae chromosomal passenger proteins Ipl1 (Aurora B) and Sli15 (INCENP) are required for the tension checkpoint, but role of third passenger, Bir1, is controversial. We have isolated a temperature-sensitive mutant (bir1-107) in essential C-terminal region Bir1 known to be binding Sli15. This allele reveals checkpoint function Bir1. displays biorientation defect, defective response lack tension, an inability detach kinetochores. localizes aberrant foci when localization...
Kinetochore attachment to the ends of dynamic microtubules is a conserved feature mitotic spindle organization that thought be critical for proper chromosome segregation. Although kinetochores have been described transition from lateral end-on attachments, phase has difficult study in yeast due its transient nature. We previously kinetochore mutant, DAM1-765, which exhibits attachments and misregulation microtubule length. Here we show length DAM1-765 cells occurs despite localization...
is a human and animal pathogen that depends on flagellar motility for transmission infection. The trypanosome flagellum built around canonical "9+2" axoneme, containing nine doublet microtubules (DMTs) surrounding two singlet microtubules. Each DMT contains 13-protofilament A-tubule 10-protofilament B-tubule, connected to the by conserved, non-tubulin inner junction (IJ) filament made up of alternating PACRG FAP20 subunits. Here we investigate in procyclic form
Abstract. We present results of electric field measurements made during the MASS rocket campaign in Andøya, Norway into noctilucent clouds (NLC) and polar mesospheric summer echoes (PMSE) on 3 August 2007. The instrument used high input-impedance preamps to measure vertical horizontal fields. No large-amplitude geophysical fields were detected cloud layers, but significant levels fluctuations measured. Within layer, probe potentials relative skin driven negative by incident heavy charged...
Trypanosoma brucei is a human and animal pathogen that depends on flagellar motility for transmission infection. The trypanosome flagellum built around canonical "9+2" axoneme, containing nine doublet microtubules (DMTs) surrounding two singlet microtubules. Each DMT contains 13-protofilament A-tubule 10-protofilament B-tubule, connected to the by conserved, non-tubulin inner junction (IJ) filament made up of alternating PACRG FAP20 subunits. Here we investigate in procyclic form T. . A...
We present a lensless time-resolved speckle imaging technique for label-free, sensitive and rapid detection of motile parasites in bodily fluids, by using the locomotion as biomarker contrast mechanism.
ABSTRACT Trypanosoma brucei is the protozoan parasite responsible for sleeping sickness, a lethal vector-borne disease. T. has single flagellum that plays critical roles in biology, transmission and pathogenesis. An emerging concept biology organelle organized into subdomains, each having specialized composition function. Overall proteome been well-studied, but gap knowledge protein of individual subdomains. We have therefore used APEX-based proximity proteomics to examine To assess...