A5052865089- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Additive Manufacturing and 3D Printing Technologies
- Total Knee Arthroplasty Outcomes
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Knee injuries and reconstruction techniques
- E-commerce and Technology Innovations
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Cardiac electrophysiology and arrhythmias
- Viral Infectious Diseases and Gene Expression in Insects
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Inflammatory mediators and NSAID effects
- Cellular Mechanics and Interactions
- Cardiac Fibrosis and Remodeling
Politecnico di Milano
2015-2025
University of Basel
2016-2020
University Hospital of Basel
2016-2020
Charlottesville Medical Research
2019
John Wiley & Sons (United States)
2019
Wake Forest University
2019
Forest Institute
2019
The University of Queensland
2016
Brisbane School of Theology
2016
Mylan (South Africa)
2015
The beating heart-on-a-chip (i) generates 3D cardiac constructs with well-defined geometries from cell-laden hydrogel prepolymers, (ii) provides uniaxial cyclic mechanical stimulation, (iii) allows efficient delivery of drugs and chemicals.
Patient responses to immune checkpoint inhibitors can be influenced by the gastrointestinal microbiome. Mouse models used study microbiome–host crosstalk, yet their utility is constrained substantial anatomical, functional, immunological and microbial differences between mice humans. Here we show that a gut-on-a-chip system mimicking architecture functionality of human intestine including faecal microbiome peristaltic-like movements recapitulates interactions predicts in patients with...
Abstract The ability to replicate in vitro the native extracellular matrix (ECM) features and control three‐dimensional (3D) cell organization plays a fundamental role obtaining functional engineered bioconstructs. In tissue engineering (TE) applications, hydrogels have been successfully implied as biomatrices for 3D embedding, exhibiting high similarities natural ECM holding easily tunable mechanical properties. present study, we characterized promising photocrosslinking process generate...
Abstract The development of in vitro models to screen the effect different concentrations, combinations and temporal sequences morpho-regulatory factors on stem/progenitor cells is crucial investigate possibly recapitulate developmental processes with adult cells. Here, we designed validated a microfluidic platform (i) allow cellular condensation, (ii) culture 3D micromasses human bone marrow-derived mesenchymal stromal (hBM-MSCs) under continuous flow perfusion deliver defined...
Significance The study offers a pharmacological solution to the challenging target of inducing stable chondrogenesis by human mesenchymal stromal cells (hMSCs), including protection against vascularization. Cells were reversed from tendency follow default differentiation pathway, namely endochondral ossification and osteogenesis. Our findings open perspectives in articular cartilage regeneration, as well establishment hMSC-based models development, physiology, possibly pathology....
Native tissues are characterized by spatially organized three-dimensional (3D) microscaled units which functionally define cells–cells and cells–extracellular matrix interactions. The ability to engineer biomimetic constructs mimicking these 3D microarchitectures is subject the control over cell distribution organization. In present study we introduce a novel protocol generate laden hydrogel micropatterns with defined size shape. method, named photo-mold patterning (PMP), combines...
Organs-on-chip technology has recently emerged as a promising tool to generate advanced cardiac tissue in vitro models, by recapitulating key physiological cues of the native myocardium. Biochemical, mechanical, and electrical stimuli have been investigated demonstrated enhance maturation constructs. However, combined application such stimulations on 3D organized constructs within microfluidic platform was not yet achieved. For this purpose, we developed an innovative microbioreactor...
Osteoarthritis (OA) is the most prevalent joint disorder, causing pain and disability predominantly in aging population but also affecting young individuals. Current treatments are limited to use of anti-inflammatory drugs alleviate symptoms or degenerated replacement by a prosthetic implant at end stage disease. We hypothesized that degenerative cartilage defects can be treated using nasal chondrocyte–based tissue-engineered (N-TEC). demonstrate N-TEC maintained cartilaginous properties...
Cardiac toxicity still represents a common adverse outcome causing drug attrition and post-marketing withdrawal. The development of relevantin vitromodels resembling the human heart recently opened path towards more accurate detection drug-induced cardiac early in process. Organs-on-chip have been proposed as promising tools to recapitulatein vitrothe key aspects thein vivocardiac physiology provide means directly analyze functional readouts. In this scenario, new device capable continuous...
Abstract The drug discovery and development process is still long, costly, highly risky. principal attrition factor undetected toxicity, with hepatic cardiac toxicities playing a critical role being the main responsible of safety‐related withdrawals from market. Multi Organs‐on‐Chip (MOoC) represent disruptive solution to study drug‐related effects on several organs simultaneously efficiently predict toxicity in preclinical trials. Specifically focusing safety, different technological...
Our 3D-scar-on-a-chip model resembles fibroblast proliferation and activation, extracellular matrix deposition stiffening upon application of only cyclic mechanical stretching.
Animal experimentation has been integral to drug discovery and development safety assessment for many years, since it provides insights into the mechanisms of efficacy toxicity (e.g. pharmacology, pharmacokinetics pharmacodynamics). However, due species differences in physiology, metabolism sensitivity drugs, animal models can often fail replicate effects drugs chemicals human patients, workers consumers. Researchers across globe are increasingly applying Three Rs principles by employing...
High percentages of cardiomyocytes mitigate the onset fibrotic traits induced by fibroblasts in a mid-throughput, mechanically active microdevice.
Abstract Cardiac fibrosis is one of the main causes heart failure, significantly contributing to mortality. The discovery and development effective therapies able heal fibrotic pathological symptoms thus remain paramount importance. Micro‐physiological systems (MPS) are recently introduced as promising platforms accelerate this finding. Here a 3D in vitro model human cardiac fibrosis, named uScar, developed by imposing cyclic mechanical stimulation atrial fibroblasts (AHCFs) cultured beating...
Human bone marrow derived mesenchymal stromal cells (BMSCs) represent a putative cell source candidate for tissue engineering-based strategies to repair cartilage and bone. However, traditional isolation of BMSCs by adhesion plastic leads very heterogeneous populations, accounting high variability chondrogenic differentiation outcome, both across donors clonally strains. Identification surface markers able select BMSC subpopulations with higher capacity (CC) reduced variance in could aid the...
ABSTRACT Bone morphogenetic protein (BMP) signalling plays a significant role during embryonic cartilage development and has been associated with osteoarthritis (OA) pathogenesis, being in both cases involved triggering hypertrophy. Inspired by recent findings that BMP inhibition counteracts hypertrophic differentiation of human mesenchymal progenitors, we hypothesized selective would mitigate features OA cartilage. First, 3D vitro micro-cartilage model was established using minimally...
Determining the potential cardiotoxicity and pro-arrhythmic effects of drug candidates remains one most relevant issues in development pipeline (DDP). New methods enabling to perform more representative preclinical vitro studies by exploiting induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) are under investigation increase translational power outcomes. Here we present a pharmacological campaign conducted evaluate drug-induced QT alterations arrhythmic events on uHeart, 3D...