A5002880244- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Biological Research and Disease Studies
- Mosquito-borne diseases and control
- interferon and immune responses
- Amino Acid Enzymes and Metabolism
- Force Microscopy Techniques and Applications
- Renal and related cancers
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Photoacoustic and Ultrasonic Imaging
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
- biodegradable polymer synthesis and properties
- melanin and skin pigmentation
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- Parkinson's Disease Mechanisms and Treatments
- Genetics, Bioinformatics, and Biomedical Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Coenzyme Q10 studies and effects
Université Paris Cité
2025
Inserm
2025
Institut des Maladies Génétiques Imagine
2024
German Center for Neurodegenerative Diseases
2022-2023
Hertie Institute for Clinical Brain Research
2022
University of Tübingen
2022
Patient responses to immune checkpoint inhibitors can be influenced by the gastrointestinal microbiome. Mouse models used study microbiome–host crosstalk, yet their utility is constrained substantial anatomical, functional, immunological and microbial differences between mice humans. Here we show that a gut-on-a-chip system mimicking architecture functionality of human intestine including faecal microbiome peristaltic-like movements recapitulates interactions predicts in patients with...
Abstract Mutations in GBA1 , the gene encoding lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell genomic approaches stable cell lines as well induced pluripotent stem (iPSC)-derived neurons midbrain organoids to dissect mechanisms underlying GCase-related neurodegeneration. We demonstrate that GCase can be imported from cytosol into mitochondria via...
Abstract Mitochondria have developed a specialized mitochondrial unfolded protein response (UPR mt ) to maintain proteostasis and promote recovery under stress conditions. Research in simple organisms has demonstrated that UPR activation glial cells promotes through beneficial non-cell-autonomous communication with neurons. However, the role of responses human brain remains unclear. To address this knowledge gap, we profiled cell type-specific roles using induced pluripotent stem...
This protocol describes the immunofluorescence staining of cells.
Collection of protocols Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity energy metabolism"
This protocol details about single cell dissociation of brain organoids.
Expansion microscopy is a technique to visualize biological structures with higher spatial resolution than traditional methods.
Soluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the protein based on their solubility properties.
Fontana-Masson staining is a silver technique that commonly used to identify melanin-containing cells. We combined it with DAB-TH works by using an antibody detect the presence of TH, followed reaction substrate (DAB) results in formation brown-colored product at site antigen-antibody interaction.
Collection of protocols Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity energy metabolism"
In this protocol we describe the differentiation of human induced pluripotent stem cells (hiPSCs) into midbrain-like organoids (hMLOs). This has been developed based from several published protocols.
Abstract Mutations in the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher's disease, are most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employed global proteomic and single-cell genomic approaches stable cell lines as well induced pluripotent stem (iPSC)-derived neurons midbrain organoids to dissect mechanisms underlying GCase-related neurodegeneration. We demonstrate that a fraction of GCase can be imported from cytosol into mitochondria via...
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"
In this protocol we describe the differentiation of human induced pluripotent stem cells (hiPSCs) into midbrain-like organoids (hMLOs). This has been developed based from several published protocols.
Collection of protocols Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly function"