A5087006950- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Neuroscience and Neural Engineering
- Virus-based gene therapy research
- Advancements in Transdermal Drug Delivery
- Image Processing Techniques and Applications
- Circular RNAs in diseases
- Ocular Infections and Treatments
- Extracellular vesicles in disease
Polytechnic University of Turin
2018-2024
University of Turin
2019
Modulation of microRNA expression holds the promise to achieve direct reprogramming fibroblasts into cardiomyocyte-like cells as a new strategy for myocardial regeneration after ischemic heart disease. Previous reports have shown that murine can be directly reprogrammed induced cardiomyocytes (iCMs) by transient transfection with four mimics (miR-1, 133, 208 and 499, termed "miRcombo"). Hence, study on effect miRcombo adult human cardiac (AHCFs) deserves attention in perspective future...
Design of nanocarriers for efficient miRNA delivery can significantly improve miRNA-based therapies. Lipoplexes based on helper lipid, dioleoyl phosphatidylethanolamine (DOPE) and cationic lipid [2-(2,3-didodecyloxypropyl)-hydroxyethyl] ammonium bromide (DE) were formulated to efficiently deliver miR-1 or a combination four microRNAs (miRcombo) adult human cardiac fibroblasts (AHCFs). with amino-to-phosphate groups ratio 3 (N/P 3) showed nanometric hydrodynamic size (372 nm), positive...
Abstract Cardiac fibrosis is one of the main causes heart failure, significantly contributing to mortality. The discovery and development effective therapies able heal fibrotic pathological symptoms thus remain paramount importance. Micro‐physiological systems (MPS) are recently introduced as promising platforms accelerate this finding. Here a 3D in vitro model human cardiac fibrosis, named uScar, developed by imposing cyclic mechanical stimulation atrial fibroblasts (AHCFs) cultured beating...
The restoration of cardiac functionality after myocardial infarction represents a major clinical challenge. Recently, we found that transient transfection with microRNA combination (miRcombo: miR-1, miR-133, miR-208 and 499) is able to trigger direct reprogramming adult human fibroblasts (AHCFs) into induced cardiomyocytes (iCMs) in vitro. However, achieving efficient still remains aim this study was investigate the influence tissue-like biochemical biophysical stimuli on efficiency....
Abstract Direct reprogramming of fibroblasts into induced cardiomyocytes (iCMs) through microRNAs (miRNAs) is a new emerging strategy for myocardial regeneration after ischemic heart disease. Previous studies have reported that murine can be directly reprogrammed iCMs by transient transfection with four miRNAs (miRs-1, 133, 208 and 499 – termed “miRcombo”). While advancement in the knowledge direct cell molecular mechanism progress, it important to investigate if this may translated humans....
Cardiovascular diseases represent a worldwide social and economic challenge, due to heart tissue limited regenerative capabilities. 3D bioprinted cell-laden constructs are promising approach develop patches for cardiac regeneration or in vitro models new drug preclinical discovery validation. However, the development of bioinks with optimal mechanical, rheological, biological properties is still challenge. Although alginate (Alg)-based have been extensively explored, such hydrogels lack cell...
Skin infections are currently a worldwide emergency as antibiotic-resistant bacteria spreading, leading to the ineffectiveness of most antibiotics and antibacterial strategies. Consequently, there is an urgency developing testing innovative therapies. As traditional 2D cell culture planktonic can be obsolete due their incapability resembling complex infection environment, 3D in vitro skin models powerful tool test validate In this article, epidermis–dermis model has been developed...
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