A5063131456- Photosynthetic Processes and Mechanisms
- Quantum Dots Synthesis And Properties
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Light effects on plants
- Metal-Catalyzed Oxygenation Mechanisms
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Porphyrin and Phthalocyanine Chemistry
- Spectroscopy and Quantum Chemical Studies
- Lipid Membrane Structure and Behavior
- Carbon and Quantum Dots Applications
- Chalcogenide Semiconductor Thin Films
- Metalloenzymes and iron-sulfur proteins
- Biocrusts and Microbial Ecology
- Legume Nitrogen Fixing Symbiosis
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- Mitochondrial Function and Pathology
- Advanced Biosensing Techniques and Applications
- Nanocluster Synthesis and Applications
- Energy Harvesting in Wireless Networks
- Plant Molecular Biology Research
- Lipid metabolism and biosynthesis
- Plant and animal studies
University of Wrocław
2017-2024
Institute of Physics
2012-2019
Polish Academy of Sciences
2010-2019
University of Oxford
2018
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2018
Institute of Geophysics
2018
Czech Academy of Sciences, Institute of Physics
2012-2015
Jagiellonian University
2003-2013
Weizmann Institute of Science
2008-2011
Johannes Gutenberg University Mainz
2005
In this study, we have examined the influence of different lipids on solubility xanthophyll cycle pigments diadinoxanthin (Ddx) and violaxanthin (Vx) efficiency Ddx Vx de-epoxidation by enzymes de-epoxidase (VDE) from wheat (DDE) diatom Cyclotella meneghiniana, respectively. Our results show that MGDG PE are able to solubilize both in an aqueous medium. Substrate solubilization is essential for activity, because absence or present aggregated form, with limited accessibility DDE VDE. also...
Ultraviolet B (UV-B) irradiation can influence many cellular processes. Irradiation with high UV-B doses causes chlorophyll degradation, a decrease in the expression of genes associated photosynthesis and its subsequent inhibition. On other hand, sublethal are used post-harvest technology to prevent yellowing storage. To address this inconsistency effect short, high-dose on detached Arabidopsis thaliana leaves was examined.
Photosynthetic organisms utilize interacting pairs of chlorophylls and bacteriochlorophylls as excitation energy donors acceptors in light harvesting complexes, photosensitizers charge separation reaction centers, maybe photoprotective quenching centers that dissipate excess under high intensities. To better understand how the pigment's local environment spatial organization within protein tune its ground- excited-state properties to perform different functions, we prepared characterized...
The synthesis and assembly of functioning photosynthetic complexes in chloroplasts developing from etioplasts during the de-etiolation angiosperm seedlings are imperative for plant's autotrophic lifestyle. This study compared process under monochromatic red or blue light equal photon flux density a 24-hour illumination period etiolated Arabidopsis seedlings. aim was to elucidate impact these wavelength on etioplast-to-chloroplast transformation initiation light-dependent reactions. Both...
In the present studies, we have found a fragment of amino acid sequence, called TFT motif, both in light-dependent protochlorophyllide oxidoreductase (LPOR) and L subunit dark-operative (light-independent) oxidoreductases (DPOR). Amino residues this motif shared similar physicochemical properties types enzymes. paper, common its spatial arrangement possible physiological role are being discussed. This is first report when similarity between LPOR DPOR, phylogenetically unrelated, but...
Colloidal quantum dots (QDs) are nanoparticles that able to photoreduce redox proteins by electron transfer (ET). QDs also energy resonance (RET). Here, we address the question of competition between these two routes QDs' excitation quenching, using cadmium telluride and cytochrome c (CytC) or its metal-substituted derivatives. We used both oxidized reduced versions native CytC, as well fluorescent, nonreducible Zn(II)CytC, Sn(II)CytC, metal-free porphyrin CytC. found all CytC quench QD...
This paper reports the observation of Tb(3+) 4f-4f emission gain in ZrO2 nanocrystals stabilized by Y2O3 as amount stabilizer increases from 0% to 10% mol. The were obtained via microwave solvothermal technology. photoluminescence properties as-grown samples are investigated. possibility biological applications material is tested on living organisms (mice). result indicates potential use studied a luminescent nanomarker.
While quantum dots (QDs) are useful as fluorescent labels, their application in biosciences is limited due to the stability and hydrophobicity of surface. In this study, we tested two types proteins for use a cover spherical QDs, composed cadmium selenide. Pumilio homology domain (Puf), which mostly α-helical, leucine-rich repeat (LRR) domain, rich β-sheets, were selected determine if there preference one these secondary structure nanoparticle covers. The protein sequences optimized improve...
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the first step of carbon fixation performed by photosynthetic organisms. Form I this enzyme found in plants and cyanobacteria is composed eight large (RbcL) small (RbcS) subunits. To form a functional enzyme, Rubisco subunits need to be properly folded, with assistance cellular chaperone machinery, consecutively assembled strictly orchestrated manner, help multiple auxiliary factors. In recent years, assembly chaperones...
Ferredoxin:NADP+ oxidoreductase (FNR) is a plant and cyanobacterial photosynthetic enzyme, also found in non-photosynthetic tissues, where it involved redox reactions of biosynthetic pathways. In vivo transfers electrons to nicotinamide adenine dinucleotide phosphate (NADP+), forming its reduced version, NADPH, while vitro can use NADPH reduce several substrates, such as ferricyanide, various quinones nitriles. As an catalyzing reaction broad range FNR may be used biotechnological processes....
Colloidal CdTe quantum dots may photoreduce both heme and iron–sulfur cluster containing proteins. Reduction level be tuned by choosing different size of nanocrystals.