A5085621206- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Quantum Dots Synthesis And Properties
- Biochemical and Molecular Research
- interferon and immune responses
- DNA and Nucleic Acid Chemistry
- Biotin and Related Studies
- PI3K/AKT/mTOR signaling in cancer
- Biochemical Acid Research Studies
- Carbon and Quantum Dots Applications
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Anodic Oxide Films and Nanostructures
- nanoparticles nucleation surface interactions
- Chemical Synthesis and Characterization
- Chemical Synthesis and Analysis
- Drug Transport and Resistance Mechanisms
- Bone health and treatments
- Inorganic Fluorides and Related Compounds
- Photoacoustic and Ultrasonic Imaging
- Ferrocene Chemistry and Applications
- CRISPR and Genetic Engineering
- Polyamine Metabolism and Applications
University of Warsaw
2006-2020
Fraunhofer Institute for Cell Therapy and Immunology
2017-2020
University of Oklahoma
2019
MmpL3, an essential transporter involved in the export of mycolic acids, is proposed target a number antimycobacterial inhibitors under development. Whether MmpL3 serves as direct these compounds, however, has been called into question after discovery that some them dissipated proton motive force from which MmpL transporters derive their energy. Using combination vitro and whole-cell-based approaches, we here provide evidence five structurally distinct inhibitor series, three impact...
Modified mRNA cap analogs aid in the study of mRNA-related processes and may enable creation novel therapeutic interventions. We report synthesis properties 11 dinucleotide bearing a single boranophosphate modification at either α-, β- or γ-position 5′,5′-triphosphate chain. The compounds can potentially serve as inhibitors translation cancer cells reagents for increasing expression proteins vivo from exogenous mRNAs. BH3-analogs were tested substrates binding partners two major cytoplasmic...
An effective and facile synthesis of six novel tetraphosphate cap analogs modified with a methylenebis(phosphonate) moiety (1–6) is presented. Analogs have been rationally designed to bind tightly the eukaryotic initiation factor 4E (eIF4E) responsible for binding during translation, increased stability owing resistance enzymatic degradation. Final compounds turned out significantly higher association constant values (KAS) eIF4E (5–9 fold than standard). Four were resistant towards...
In response to foreign RNA, cellular antiviral mechanisms stimulate high expression of interferon-induced proteins with tetratricopeptide repeats (IFITs). Two members the IFIT protein family, IFIT1 and IFIT5, are capable binding very terminal 5′ end mRNA. eukaryotes, these mRNA termini contain a cap structure (m 7 GpppN, 0) that is often subjected further modifications. Here, we performed thorough examination IFIT5 wide spectrum differently capped as well fully uncapped mRNAs. The kinetic...
The cap structure is a specific feature of the 5' end mRNA which plays an important role in post-transcriptional control gene expression. A major step regulation occurs at level turnover. Degradation most eukaryotic mRNAs entails removal various pathways. human scavenger decapping enzyme (hDcpS) catalyses cleavage residual m7GpppN and/or short oligonucleotides after exosom mediated digestion. In this paper we report fluorescence study association process hDcpS with m7GMP, m7GDP and selected...
Eukaryotic cells utilize scavenger decapping enzymes to degrade cap structure following 3′-5′ mRNA decay. Human DcpS recently has been described as a highly specific hydrolase (a member of the HIT family) that catalyses cleavage m 7 GpppG and short capped oligoribonucleotides. We have demonstrated here cap-1 (m GpppGm) is preferred substrate among several investigated dinucleotide analogues Gp n N (n = 3–5, purine or pyrimidine base) GMP always one reaction product. Cap containing base...
The syntheses of mRNA cap analogs modified with boranophosphate moiety at either the α or β-position 5′, 5′-triphosphate bridge (m7GpppBH3G, m7GppBH3pG and m7GppBH3pm7G) are described. preliminary biological characterization these compounds revealed that they have high affinity for translational factor eIF4E potency to inhibit cap-dependent translation in cell free system. were also found be resistant DcpS decapping pyrophosphatase.
The interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of RNA-binding that very highly expressed during antiviral response immune system. IFIT recognize and tightly bind foreign RNA particles. These primarily viral RNAs ended triphosphate at the 5' or lacking methylation first cap-proximal nucleotide but also in vitro transcribed synthesized laboratory. Recognition by leads to formation stable RNA/IFIT complexes translational shut off non-self transcripts. Here,...
Part A embraces all aspects of analytical cytology, which is defined broadly as characterization and measurement cells cellular constituents for biological, diagnostic, therapeutic purposes.It includes components cytochemistry, cytophysics, cell biology, molecular
A embraces all aspects of analytical cytology, which is defined broadly as characterization and measurement cells cellular constituents for biological, diagnostic, therapeutic purposes.It includes components cytochemistry, cytophysics, cell biology, molecular nanotechnology, systems biology cytomics,