A5008698649- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- RNA Research and Splicing
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- CAR-T cell therapy research
- Cancer Research and Treatments
- Influenza Virus Research Studies
- Congenital Heart Disease Studies
- Cardiac Valve Diseases and Treatments
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Sociology and Education Studies
- Cardiovascular Function and Risk Factors
- Electronic Health Records Systems
- Advanced biosensing and bioanalysis techniques
- Ethics in Clinical Research
- Immune Response and Inflammation
- Neurogenetic and Muscular Disorders Research
- Sports Science and Education
- Health and Medical Studies
BioNTech (Germany)
2015-2025
TU Dresden
2022-2023
University Hospital Carl Gustav Carus
2022-2023
University of Koblenz and Landau
2023
Deutsches Herzzentrum München
2012-2020
Charité - Universitätsmedizin Berlin
2018-2020
Johannes Gutenberg University Mainz
2010-2019
Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz
2011-2019
Heidelberg University
2008-2019
Uppsala University Hospital
2019
Multiple genetic events and subsequent clonal evolution drive carcinogenesis, making disease elimination with single-targeted drugs difficult. The multiplicity of gene mutations derived from heterogeneity therefore represents an ideal setting for multiepitope tumor vaccination. Here, we used next generation sequencing exome resequencing to identify 962 nonsynonymous somatic point in B16F10 murine melanoma cells, 563 those expressed genes. Potential driver occurred classical suppressor genes...
Synthetic mRNA has emerged as a powerful tool for the transfer of genetic information, and it is being explored variety therapeutic applications. Many these applications require prolonged intracellular persistence to improve bioavailability encoded protein. molecules are intrinsically unstable their kinetics depend on UTRs embracing coding sequence, in particular 3′ UTR elements. We describe here novel generally applicable cell-based selection process identification that augment expression...
Local immunotherapy with an mRNA mixture encoding four cytokines mobilizes a systemic antitumor response and promotes tumor eradication.
Cajal bodies (CBs) have been implicated in the nuclear phase of biogenesis spliceosomal U small ribonucleoproteins (U snRNPs). Here, we investigated distribution CB marker protein coilin, snRNPs, and proteins present C/D box nucleolar (sno)RNPs cells depleted hTGS1, SMN, or PHAX. Knockdown any these three by RNAi interferes with snRNP maturation before reentry snRNA Sm cores into nucleus. Strikingly, CBs are lost absence PHAX coilin is dispersed nucleoplasm numerous foci. This indicates that...
Abstract To contain the coronavirus disease 2019 (COVID-19) pandemic, a safe and effective vaccine against new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is urgently needed in quantities sufficient to immunise large populations. In this study, we report design, preclinical development, immunogenicity anti-viral protective effect rhesus macaques of BNT162b2 candidate. contains an LNP-formulated nucleoside-modified mRNA that encodes spike glycoprotein captured its prefusion...
Along with a growing interest in mRNA-based gene therapies, efforts are increasingly focused on reaching the full translational potential of mRNA, as major obstacle for vivo applications is sufficient expression exogenously delivered mRNA. One method to overcome this limitation chemically modifying 7-methylguanosine cap at 5' end mRNA (m7Gppp-RNA). We report novel class analogs designed reagents modification. The carry 1,2-dithiodiphosphate moiety various positions along tri- or...
The removal of intervening sequences from a primary RNA transcript is catalyzed by the spliceosome, large complex consisting five small nuclear (sn) RNAs and more than 150 proteins. At start splicing cycle, spliceosome assembles anew onto each pre-mRNA intron in an ordered process. Here, we show that several small-molecule inhibitors protein acetylation/deacetylation block cycle: testing number bioactive compounds, found three histone acetyltransferases (HATs), as well deacetylases (HDACs),...
Modified mRNA cap analogs aid in the study of mRNA-related processes and may enable creation novel therapeutic interventions. We report synthesis properties 11 dinucleotide bearing a single boranophosphate modification at either α-, β- or γ-position 5′,5′-triphosphate chain. The compounds can potentially serve as inhibitors translation cancer cells reagents for increasing expression proteins vivo from exogenous mRNAs. BH3-analogs were tested substrates binding partners two major cytoplasmic...
<h2>Abstract</h2> mRNA vaccines have been established as a safe and effective modality, thanks in large part to the expedited development approval of COVID-19 vaccines. In addition active, full-length transcript, fragment species can be present byproduct cell-free transcription manufacturing process or due hydrolysis. current study, from BNT162b2 were isolated characterized. The translational viability intact fragmented was further explored using orthogonal expression systems understand risk...
Prp8 is the largest and most highly conserved protein in spliceosome yet its mechanism of function poorly understood. Our previous studies implicate control activation for first catalytic step splicing, because substitutions five distinct regions (a–e) suppress a cold-sensitive block to caused by mutation U4 RNA. Catalytic thought require unwinding U1 RNA/5′ splice site U4/U6 RNA helices Prp28 Prp44/Brr2 DExD/H-box helicases, respectively. Here we show that mutations a, d, e exhibit...
Abstract The highly conserved splicing factor Prp8 has been implicated in multiple stages of the reaction. However, assignment a specific function to any part 280-kD U5 snRNP protein difficult, because lacks recognizable functional or structural motifs. We have used large-scale screen for Saccharomyces cerevisiae PRP8 alleles that suppress cold sensitivity caused by U4-cs1, mutant U4 RNA blocks U4/U6 unwinding, identify with high resolution five distinct regions involved control spliceosome...
Targeting mRNA to eukaryotic cells is an emerging technology for basic research and provides broad applications in cancer immunotherapy, vaccine development, protein replacement, vivo genome editing. Although a plethora of nanoparticles efficient delivery exists, targeting specific organs, tissue compartments, remains major challenge. For this reason, methods reporting the specificity different nanoparticle formats will be crucial. Here, we describe straightforward method monitoring...
The vast and heterogeneous data being constantly generated in clinics can provide great wealth for patients research alike. quickly evolving field of medical informatics has contributed numerous concepts, algorithms, standards to facilitate this development. However, these difficult relationships, complex terminologies, multiple implementations present obstacles people who want get active the field. With a particular focus on conducted Germany, we our Viewpoint set 10 important topics...