A5076541315- RNA Interference and Gene Delivery
- Analytical Chemistry and Chromatography
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Drug Solubulity and Delivery Systems
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Computational Drug Discovery Methods
- Analytical Methods in Pharmaceuticals
- Microfluidic and Capillary Electrophoresis Applications
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Biopolymer Synthesis and Applications
- Advanced Drug Delivery Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
BioNTech (Germany)
2016-2023
Al-Azhar University
2014-2017
Friedrich Schiller University Jena
2008-2010
Atorvastatin (AT) is a widely used lipid-regulating drug to reduce cholesterol and triglycerides. Its poor aqueous solubility hepatic metabolism require development of delivery systems able improve its bypass effect. For this purpose, atorvastatin nanostructured lipid carriers (AT-NLCs) were prepared characterized. AT-NLCs by emulsification using high-speed homogenization followed ultrasonication. The NLCs showed particle size between 162.5 ± 12 865.55 28 nm while zeta potential values...
The SARS-CoV-2 Omicron variant and its sublineages show pronounced viral escape from neutralizing antibodies elicited by vaccination or prior infection owing to over 30-amino acid alterations within the spike (S) glycoprotein. Breakthrough of vaccinated individuals with BA.1 BA.2 is associated distinct patterns cross-neutralizing activity against variants concern (VOCs). In continuation our previous work, we characterized effect BA.4/BA.5 S glycoprotein exposure on antibody response upon...
Abstract The retention behavior of neutral, positively charged, and negatively charged solutes on the IAM.PC.DD2 stationary phase was investigated compared. A set monofunctional compounds complex drugs (steroids, nonsteroidal anti‐inflammatory drugs, β ‐blockers) were selected for this study, i.e. , neutral with acidic or basic functionalities which are at pH 7.0. correlation between factor log k w 7.0 partition coefficient P oct distribution D showed that mechanism depends charge state...
We propose a reversed phase HPLC (RP-HPLC) with an alkaline-resistant silica-based stationary phase, XBridgeTM Shield RP18, for the determination of lipophilicity drugs diverse chemical nature ranging from acidic to basic. A set 40 model compounds well-defined solvatochromic parameters was selected allow broad distribution structural properties. The chromatographic results showed that index log kw obtained RP18 well correlated experimental Poct values (r2=0.96). Linear solvation free-energy...
Abstract The objective of this study was to investigate drug–membrane interaction by immobilized liposome chromatography (ILC; expressed as lipophilicity index log K s ) and the comparison with indices obtained liposome/H 2 O, octan‐1‐ol/H artificial membrane (IAM) systems. A set structurally diverse monofunctional compounds drugs (nonsteroidal anti‐inflammatory β ‐blockers) were selected in study. This solutes consists basic or acidic functionalities which are positively negatively charged...
Abstract The SARS-CoV-2 Omicron variant and its sublineages show pronounced viral escape from neutralizing antibodies elicited by vaccination or prior infection owing to over 30 amino acid alterations within the spike (S) glycoprotein. We others have recently reported that breakthrough of vaccinated individuals with BA.1 BA.2 are associated distinct patterns cross-neutralizing activity against variants concern (VOCs). mediated overall stronger cross-neutralization descendants (BA.2.12.1,...
Abstract Background RNA-based vaccination strategies tailoring immune response to specific reactions have become an important pillar for a broad range of applications. Recently, the use lipid-based nanoparticles opened possibility deliver RNA sites within body, overcoming limitation rapid degradation in bloodstream. Here, we investigated whether small animal PET/MRI can be employed image biodistribution RNA-encoded protein. For this purpose, reporter coding sodium-iodide-symporter (NIS) was...
Abstract Mechanisms of antiviral host defense are important for survival and evolutionarily optimized high sensitivity potency. Intending to harvest the multitude highly specialized intertwined pathogen immune programs cancer immunotherapy, we simulated a systemic intrusion into blood stream by intravenous injection lipid-formulated, tumor antigen-encoding mRNA nanoparticles. These RNA-lipoplexes (RNA-LPX) were directed various lymphoid tissues, including spleen, lymph nodes bone marrow,...
The activation of Toll-like receptors (TLR) by natural or synthetic ligands results in cytokine secretion and increased phagocytosis macrophages cytolytic activity killer (NK) cells.So,wedeveloped a stable, efficient, nontoxic cationic nanoemulsion (CNE) suitable for TLR ligand oligonucleotide (ssRNA) delivery.The is based on squalene, lipid 1,2-dioleoyl-sn-glycero-3trimethylammoniumpropane (DOTAP), helper 1,2-dioleoyl-sn-glycero-3phosphoethanolamine (DOPE), tween 80, poloxamer 188.Factorial...
Background We previously developed a saline-formulated mixture of 4 synthetic messenger ribonucleic acids (mRNAs) encoding the cytokines IL-12, IFN-α, GM-CSF, and IL-15 sushi (IL-15-IL-15Rα receptor fusion) that promoted systemic immunity tumor eradication upon intratumoral administration in multiple mouse models. Here, we explored whether stabilization mRNAs through lipoplex (LPX) formulation could confer increased expression encoded to result improved antitumor efficacy. To this end,...