A5019184925- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Polymer Surface Interaction Studies
- Antimicrobial agents and applications
- Conducting polymers and applications
- Viral gastroenteritis research and epidemiology
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neural Engineering
- Advanced Drug Delivery Systems
- Analytical Chemistry and Sensors
- Cell Adhesion Molecules Research
- Polymer crystallization and properties
- Graphene and Nanomaterials Applications
- Cancer Research and Treatments
- vaccines and immunoinformatics approaches
- Fiber-reinforced polymer composites
- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- Wound Healing and Treatments
- Bacterial biofilms and quorum sensing
- Electrochemical Analysis and Applications
BioNTech (Germany)
2020-2023
Max Planck Institute for Polymer Research
2016-2020
Johannes Gutenberg University Mainz
2018-2020
University Medical Center of the Johannes Gutenberg University Mainz
2018-2020
The globally circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern Omicron (B.1.1.529) has a large number mutations, especially in the spike protein, indicating that recognition by neutralizing antibodies may be compromised. We tested Wuhan (Wuhan-Hu-1 reference strain), Beta (B.1.351), Delta (B.1.617.2), or pseudoviruses with sera 51 participants who received two three doses messenger RNA (mRNA)-based COVID-19 vaccine BNT162b2. After doses,...
BNT162b2, a lipid nanoparticle (LNP) formulated nucleoside-modified messenger RNA (mRNA) encoding the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S) stabilized in prefusion conformation, has demonstrated 95% efficacy to prevent disease 2019 (COVID-19). Recently, we reported preliminary BNT162b2 safety and antibody response data from an ongoing placebo-controlled, observer-blinded phase 1/2 vaccine trial 1 . We present here T cell responses second,...
Biofilm formation is most commonly combatted with antibiotics or biocides. However, proven toxicity and increasing resistance of bacteria increase the need for alternative strategies to prevent adhesion surfaces. Chemical modification surfaces by tethering functional polymer brushes films provides a route toward antifouling coatings. Furthermore, nanorough superhydrophobic can delay biofilm formation. Here we show that submicrometer-sized roughness outweigh surface chemistry testing E. coli...
Materials with a hierarchical structure often demonstrate superior properties combined and even synergistic effects of multiple functions. Herein, we report the design new class material multicompartment nanofibrous as promising candidate for antibacterial wound dressing functional textile applications. The consists in first synthesizing nanocapsules loaded payloads subsequently embedding into polymer nanofibers by using colloid-electrospinning technique. nanocontainer-in-nanofiber allows...
The presence of the cap structure on 5'-end in vitro-transcribed (IVT) mRNA determines its translation and stability, underpinning use therapeutics. Both enzymatic co-transcriptional capping may lead to incomplete positioning newly synthesized RNA molecules. IVT mRNAs are rapidly emerging as novel biologics, including recent vaccines against COVID-19 vaccine candidates other infectious diseases, well for cancer immunotherapies protein replacement therapies. Quality control methods necessary...
The SARS-CoV-2 Omicron variant and its sublineages show pronounced viral escape from neutralizing antibodies elicited by vaccination or prior infection owing to over 30-amino acid alterations within the spike (S) glycoprotein. Breakthrough of vaccinated individuals with BA.1 BA.2 is associated distinct patterns cross-neutralizing activity against variants concern (VOCs). In continuation our previous work, we characterized effect BA.4/BA.5 S glycoprotein exposure on antibody response upon...
Electrodes coated with poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) have been employed to measure the integrity of cellular barriers. However, a systematic experimental study correlation between tissue and impedance sensing device has not yet conducted. Using spectroscopy, how ratio biological recording affects ability latter is investigated. PEDOT:PSS-coated electrodes various dimensions are effect their size efficiency examined. The biotic/abiotic ensemble modeled...
Abstract A new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage, B.1.1.529, was recently detected in Botswana and South Africa is now circulating globally. Just two days after it first reported to the World Health Organization (WHO), this strain classified as a variant of concern (VOC) named Omicron. Omicron has an unusually large number mutations, including up 39 amino acid modifications spike (S) protein, raising concerns that its recognition by neutralizing antibodies...
Abstract The integrity of CaCo‐2 cell barriers is investigated by organic electrochemical transistors (OECTs) in a current‐driven configuration. Ion transport through cellular via the paracellular pathway modulated tight junctions between adjacent cells. Rupturing its H 2 O monitored change output voltage transfer characteristics. It demonstrated that operating OECT configuration, sensitive and temporal resolution for monitoring barrier strongly enhanced as compared to transient response...
Argininosuccinic aciduria (ASA) is a metabolic disorder caused by deficiency in argininosuccinate lyase (ASL), which cleaves argininosuccinic acid to arginine and fumarate the urea cycle. ASL (ASLD) leads hepatocyte dysfunction, hyperammonemia, encephalopathy, respiratory alkalosis. Here we describe novel therapeutic approach for treating ASA, based on nucleoside-modified messenger RNA (modRNA) formulated lipid nanoparticles (LNP). To optimize ASL-encoding mRNA, modified its cap, 5' 3'...
Abstract The SARS-CoV-2 Omicron variant and its sublineages show pronounced viral escape from neutralizing antibodies elicited by vaccination or prior infection owing to over 30 amino acid alterations within the spike (S) glycoprotein. We others have recently reported that breakthrough of vaccinated individuals with BA.1 BA.2 are associated distinct patterns cross-neutralizing activity against variants concern (VOCs). mediated overall stronger cross-neutralization descendants (BA.2.12.1,...
Aim: For vaccines the combination between an antigen and adjuvants are both crucially important to trigger effective immune response in dendritic cells. Innovative like resiquimod or muramyldipeptide have their target protein inside cell. Materials & methods: Up/downregulation proteome expression was investigated for adjuvant a soluble form versus encapsulated into nanocarrier. Results: We found that 1225 genes were upregulated after nanocarrier treatment while 478 downregulated. Most...