A5085623233- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Glioma Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- Advanced Drug Delivery Systems
- Polymer Surface Interaction Studies
- Cancer Immunotherapy and Biomarkers
- Neuroblastoma Research and Treatments
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Bio-sensing Technologies
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Electrospun Nanofibers in Biomedical Applications
- Synthesis and Biological Evaluation
Johannes Gutenberg University Mainz
2015-2024
Centrum für Integrierte Onkologie
2020
University Medical Center of the Johannes Gutenberg University Mainz
2015-2018
Max Planck Society
2015-2017
Max Planck Institute for Polymer Research
2015-2017
Dendritic cells (DCs) are key regulators of protective immune responses and tolerance to (self-)Ags. Therefore, the scientific rationale for use tolerogenic DC therapy in fields allergies, autoimmunity, transplantation medicine is strong. In this study, we analyzed capacity IL-10-modulated (IL-10DC) subpopulations identify a subset that combines potent immunosuppressive activities with valuable properties clinical implementation. IL-10DCs consist two phenotypically distinct subpopulations:...
A major demand on immunotherapy is the direct interference with specific immune cells in vivo. In contrast to antibody-engineered nanoparticles control dendritic function, targeting of T for biomedical applications still remains an obstacle as they disclose reduced endocytic activities. Here, by coupling cytokine interleukin-2 (IL-2) surface hydroxyethyl starch nanocapsules, we demonstrated a and specifc cell vitro vivo IL-2 receptor-mediated internalization. For this purpose, defined...
The attachment of two different functionalities in a site-selective fashion represents great challenge protein chemistry. We report site specific dual functionalizations peptides and proteins capitalizing on reactivity differences cysteines their free (thiol) protected, oxidized (disulfide) forms. functionalization interleukin 2 EYFP proceeded with no loss bioactivity stepwise applying maleimide disulfide rebridging allyl-sulfone groups. In order to ensure broader applicability the strategy,...
Background: The prognosis of children with diffuse intrinsic pontine glioma (DIPG) remains dismal despite radio- and chemotherapy or molecular-targeted therapy. Immunotherapy is a powerful promising approach for improving the overall survival (OS) DIPG. Methods: A retrospective analysis feasibility, immune responsiveness, OS was performed on 41 treated in compassionate use multimodal therapy consisting Newcastle disease virus, hyperthermia, autologous dendritic cell vaccines as part an...
Nanocarrier synthesis is highly process-dependent, leading to potential batch-to-batch variability if it not controlled at each step. This affects the reproducibility of subsequent biomodification, resulting in unpredictable biological effects, particularly for bioactive molecules such as interleukin-2 (IL-2). Inconsistent conjugation can lead variable treatment outcomes and severe side effects. Therefore, precise control step critical ensuring a consistent quality performance. Our study...
Abstract Cell type specific delivery of drugs by use nanocapsules (NC) is a promising approach for induction efficient anti-tumor immunotherapies. Here, we report the generation IL-2 functionalized NC (HES-IL-2) to target CD25+ (IL-2 receptor α chain) T cells. In flow cytometry experiments HES-IL-2 exhibited an enhanced uptake human cells compared control capsules. The observed was CD25 as CD25high displayed significantly incorporation CD25− with negligible internalization. addition blocking...
Abstract The prognosis of children with Diffuse Intrinsic Pontine Glioma (DIPG) remains dismal in spite radio- and chemotherapy or therapies based on molecular biology diagnostics. Immunotherapy is a powerful promising approach for improving the overall survival (OS). A retrospective analysis feasibility, immune responsiveness OS was performed 41 treated compassionate use Newcastle Disease Virus, hyperthermia autologous dendritic cell vaccines as part an individualized combined treatment...