A5022216306- Luminescence and Fluorescent Materials
- Advanced Fluorescence Microscopy Techniques
- Analytical Chemistry and Sensors
- Molecular Sensors and Ion Detection
- Porphyrin and Phthalocyanine Chemistry
- Photochemistry and Electron Transfer Studies
- Protein Interaction Studies and Fluorescence Analysis
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Advanced X-ray and CT Imaging
- Diamond and Carbon-based Materials Research
- Medical Imaging Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Advanced Surface Polishing Techniques
- Photodynamic Therapy Research Studies
- Supramolecular Chemistry and Complexes
- Biosensors and Analytical Detection
- Electron Spin Resonance Studies
- Advanced Optical Sensing Technologies
- Ionic liquids properties and applications
- Optical Imaging and Spectroscopy Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Organic Light-Emitting Diodes Research
- Photoreceptor and optogenetics research
- Spectroscopy Techniques in Biomedical and Chemical Research
Texas Christian University
2014-2024
Fort Worth Library
2014-2016
Fluorescence properties of a novel homodimeric BODIPY dye rotor for Lifetime Imaging Microscopy (FLIM) are reported.
Fluorescence technologies have been the preferred method for detection, analytical sensing, medical diagnostics, biotechnology, imaging, and gene expression many years. becomes essential studying molecular processes with high specificity sensitivity through a variety of biological processes. A significant problem practical fluorescence applications is apparent non-linearity intensity resulting from inner-filter effects, sample scattering, absorption intrinsic components samples. Sample can...
Abstract Fluorescence is an established technology for studying molecular processes and interactions. More recently fluorescence became a leading method detection, sensing, medical diagnostics, biotechnology, imaging, DNA analysis, gene expression. Consequently, precise accurate measurements in various conditions have become more critical proper result interpretations. Previously, Part 1, we discussed inner filter effect type I, which consequence of the instrumental geometrical sensitivity...
Photophysical behaviour of a novel trimeric BODIPY rotor with high extinction coefficient is reported. Steady state and time resolved fluorescence measurements established that the trimer could be used as viscometer for molecular solvents, membrane-like environments several cancer cell lines.
The fluorescent lifetimes of the BODIPY–BODIPY dyad appear to correlate with viscosity media, thus making this rotor a suitable small molecule viscometer.
Structure of the cationic and anionic counterparts ionic liquids has a significant impact on conformational bias porphyrin rotor; an apparent correlation between conformation viscosity was noted, albeit it found to be distinct more complex from that in molecular solvents.
We studied room temperature phosphorescence of tryptophan (TRP) embedded in poly (vinyl alcohol) films. With UV (285 nm) excitation, the spectrum appears at about 460 nm. also observed TRP with blue light excitation 410 nm, well outside S0→S1absorption. This reaches triplet state directly without involvement singlet excited state. The lifetime is sub-millisecond range. long-wavelength direct to results high anisotropy which can be useful macromolecule dynamics study via time-resolved phosphorescence.
The signal-to-background ratio is the limiting factor for fluorescence based detection, sensing, and imaging. A typical background signal will include direct scattering of excitation Raman sample as well autofluorescence from additives. To improve ratio, fluorophores high brightness and/or concentration need to be used. Most instantaneous short-lived (picosecond nanosecond time scale), using long-lived probes combined with time-gated detection allows significant suppression unwanted...
We report the spectral properties of 2-Phenylindole (2PI) embedded in rigid poly (vinyl alcohol) (PVA) film. The 2PI PVA film shows relatively strong and structured fluorescence with a maximum at 370 nm surprisingly room temperature phosphorescence an emission about 500 nm. dye is highly immobilized polymer matrix, thus presenting high anisotropy isotropic 0.3 temperature. excited usual way through electronic singlet state excitation (S0 → S1 absorption) results very low, near zero...
Fluorescence signal enhancement induced by the binding of intercalators to DNA has been broadly utilized in various detection methods. In most instances increase fluorescence intensity is associated with a concomitant lifetime. This lifetime presents an additional opportunity sensitivity. this paper, we present new approach significantly enhance sensitivity detecting minute concentrations. The based on simultaneous use time-gated and multi-pulse pumping. By using calibrated burst short...
This article presents a novel approach to increase the detection sensitivity of trace amounts DNA in sample by employing Förster resonance energy transfer (FRET) between intercalating dyes. Two intercalators that present efficient FRET were used enhance and improve specificity detecting minute DNA. Comparison steady-state acceptor emission spectra with without donor allows for simple specific (acceptor bound DNA) down 100 pg/μL. When utilizing as an dye significantly longer lifetime (e.g.,...
We present a comprehensive spectroscopic study supported by theoretical quantum chemical calculations conducted on molecular system (4-(5-methyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (C1) and the antibiotic Amphotericin B (AmB)) that exhibits highly synergistic properties. previously reported strong synergism of this now wish to related stationary measurements UV-Vis absorption, fluorescence, fluorescence anisotropy in polar, aprotic solvent (DMSO PBS buffer), followed time-resolved...
Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced imaging application fluorescent bio-nano probe using multipulse excitation scheme. Multipulse takes advantage long lifetime (> 1µs) and enhances its intensity 15 times over short lived cellular auto-fluorescence. Moreover have also shown that by time gated detection strategy signal (fluorescence clusters) noise (auto-fluorescence)...