A5000604408- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Quantum Dots Synthesis And Properties
- Luminescence and Fluorescent Materials
- Chalcogenide Semiconductor Thin Films
- Nanocluster Synthesis and Applications
- Advanced Fluorescence Microscopy Techniques
- Cardiomyopathy and Myosin Studies
- Advanced Nanomaterials in Catalysis
- Cardiovascular Function and Risk Factors
- Lanthanide and Transition Metal Complexes
- Advanced biosensing and bioanalysis techniques
- Protein Interaction Studies and Fluorescence Analysis
- Photochemistry and Electron Transfer Studies
- Molecular Sensors and Ion Detection
- Cardiovascular Effects of Exercise
- Plasmonic and Surface Plasmon Research
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Medical Imaging Techniques and Applications
- Cancer, Lipids, and Metabolism
- Photoreceptor and optogenetics research
- Radiopharmaceutical Chemistry and Applications
- Porphyrin and Phthalocyanine Chemistry
- Advanced X-ray and CT Imaging
University of North Texas Health Science Center
2015-2025
University of North Texas
2015-2025
Imperial Consultants
2022
Texas Christian University
2014-2021
National Heart Lung and Blood Institute
2020
National Institutes of Health
2020
Fort Worth Library
2014-2018
Institut des Maladies Métaboliques et Cardiovasculaires
2017
Jan Kochanowski University
2011
University of Copenhagen
2010
Fluorescence properties of a novel homodimeric BODIPY dye rotor for Lifetime Imaging Microscopy (FLIM) are reported.
Bovine serum albumin (BSA) protected nanoclusters (Au and Ag) represent a group of nanomaterials that holds great promise in biophysical applications due to their unique fluorescence properties lack toxicity. These metal have utility variety disciplines including catalysis, biosensing, photonics, imaging molecular electronics. However, they suffer from several disadvantages such as low quantum efficiency (typically near 6%) broad emission spectrum (540 nm 800 nm). We describe an approach...
This work reports the surface functionalization of polymeric PLGA nanoparticles by non-covalent insertion a homo-bifunctional chemical crosslinker, bis(sulfosuccinimidyl) suberate (BS3) for targeted cancer therapy. We dissolved BS3 in aqueous solution PVA during formulation modified solid/oil/water emulsion solvent evaporation method. The was confirmed Fourier transform infrared (FTIR) spectroscopy. Curcumin and annexin A2 were used as model drug cell specific target, respectively....
The fluorescence lifetimes of most red emitting organic probes are under 4 nanoseconds, which is a limiting factor in studying interactions and conformational dynamics macromolecules. In addition, the nanosecond background autofluorescence significant interference during measurements cellular environment. Therefore, fluorophores with longer will be immensely helpful. Azaoxa-triangulenium ADOTA DAOTA small molecules high quantum yield, long lifetime anisotropy. aqueous environment, absorption...
BSA protected gold nanoclusters (Au25) are attracting a great deal of attention due to their unique spectroscopic properties and possible use in biophysical applications. Although there reports on synthetic strategies, spectroscopy applications, little is known about polarization behavior. In this study, we synthesized the Au25 studied steady state time resolved fluorescence including behavior different solvents: glycerol, propylene glycol water. We demonstrated that nanocluster absorption...
This work reports on the chromophores interactions within protein-protected gold nanoclusters. We conducted spectroscopic studies of fluorescence emissions originated from nanoclusters and intrinsic tryptophan (Trp) in BSA or HSA proteins. Both, steady state lifetime measurements show a significant Forster resonance energy transfer (FRET) Trp to nanocluster. Tryptophan lifetimes case are 2.6ns 2.3ns for Au clusters while 5.8ns native 5.6 HSA. The apparent distances nanocluster emission...
Curcumin, a naturally occurring yellow-orange pigment with potent antioxidant and antitumor properties, has been attracting researchers from wide range of fields including chemistry, spectroscopy, biology, medicine. Ultrafast excited-state processes such as solvation intramolecular hydrogen atom transfer (ESIHT) make curcumin an attractive agent for photodynamic therapy. In this report we present studies linear dichroism fluorescence anisotropy in oriented isotropic media. The results show...
In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH temperature on fluorescence properties tryptophan molecule nanocluster native BSA. We also their peak emission (650 nm). The photophysical characterization a newly developed fluorophore in different environments is absolutely necessary to futher develop biomedical analytical applications. It was observed from our experiments that better shielded...
Time-resolved and temperature-dependent photoluminescence (PL) spectroscopy of ternary compound copper indium disulfide (CuInS2, or CIS) core materials, CIS/ZnS coreshells, quaternary ZnCuInS2 (ZnCIS) revealed their optical properties with spectral, temporal, thermal characteristics, which were closely linked to surface-related recombination, shallow deep defect-related donor-acceptor transitions. The PL peaks semiconductor nanocrystals (SNCs) sizes near Bohr radius displayed at ∼775 nm for...
The work presented here reports the use of long lifetime (>1 μs) BSA Au clusters as a cellular/tissue, time gated, intensity imaging probe. By collecting emission signal 50 ns post excitation, one can off-gate intense auto-fluorescence background, thereby greatly enhancing clarity/specificity in fluorescence imaging.
Abstract: Current cancer chemotherapy is frequently associated with short- and long-term side effects, affecting the quality of life survivors. Because malignant cells are known to overexpress specific surface antigens, including receptors, targeted drug delivery often utilized reduce or overcome effects. The current study involves a novel targeting approach using specifically designed nanoparticles, encapsulation anti-cancer valrubicin into superparamagnetic iron oxide nanoparticle (SPION)...
Photophysical behaviour of a novel trimeric BODIPY rotor with high extinction coefficient is reported. Steady state and time resolved fluorescence measurements established that the trimer could be used as viscometer for molecular solvents, membrane-like environments several cancer cell lines.
The goal of these studies was to provide proof concept for a novel targeted therapy Glioblastoma Multiforme (GBM). Methods. These involve the evaluation reconstituted high density lipoprotein (rHDL) nanoparticles (NPs) as delivery agents drug, mammalian Target Rapamycin (mTOR) inhibitor Everolimus (EVR) GBM cells. Cytotoxicity and assessment downstream effects, including apoptosis, migration, cell cycle events, were probed, in relation expression scavenger receptor B type 1 (SR-B1) by...
This work reports the targeting of near infrared (NIR) dye indocyanine green (ICG) to brain using composite nanoparticles. Thermal decomposition iron pentacarbonyl was used synthesize monodisperse oleic acid coated magnetic nanoparticles (OAMNP). Synthesized OAMNP and ICG were encapsulated in a poly (lactide-co-glycolide) matrix an emulsion evaporation method. Different batches containing OAMNP:PLGA ratios (1:4, 1:2 3:4) prepared with (group B-1, 2, 3) without A-1, loading. All formulations...
LDS 798; a known solvatochromic probe is responsive to micro-viscosity and pH changes. Spectral changes can be used polarity while lifetime/intensity change micro-viscosity.
Stretching a polymer film induces conformational change (from the twisted to planar state) in embedded porphyrin dimer, as evidenced by steady-state and time-resolved emission spectra.
The fluorescent lifetimes of the BODIPY–BODIPY dyad appear to correlate with viscosity media, thus making this rotor a suitable small molecule viscometer.