A5087786463- Folate and B Vitamins Research
- Parasite Biology and Host Interactions
- Metabolism and Genetic Disorders
- Parasites and Host Interactions
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Alcoholism and Thiamine Deficiency
- RNA modifications and cancer
- Research on Leishmaniasis Studies
- Biochemical and Molecular Research
- Insect Resistance and Genetics
- Parasitic Infections and Diagnostics
- Diabetes and associated disorders
- DNA and Nucleic Acid Chemistry
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Diabetes Management and Research
- Nematode management and characterization studies
- Helminth infection and control
University of Colorado Denver
2012-2024
Autonomous Healthcare
2023
University of Iowa
2016-2020
The University of Texas MD Anderson Cancer Center
2012-2019
University of Utah
2019
The Medical Center of Aurora
2014
University of Colorado Anschutz Medical Campus
1985-2013
University of Colorado Boulder
2013
Air Force Medical University
2012
Lymphoma Research Foundation
2004-2012
Eukaryotic cells express several classes of small RNAs that regulate gene expression and ensure genome maintenance. Endogenous siRNAs (endo-siRNAs) Piwi-interacting (piRNAs) mainly control transposon in the germline, while microRNAs (miRNAs) generally function post-transcriptional silencing both somatic germline cells. To provide an evolutionary developmental perspective on RNA pathways nematodes, we identified characterized known novel through gametogenesis embryo development parasitic...
PRMT5 (protein arginine methyltransferase 5) is an enzyme that catalyses transfer of methyl groups from S-adenosyl methionine to the residues histones or non-histone proteins and involved in a variety cellular processes. Although it highly expressed some tumours, its direct role cancer growth has not been fully investigated. In present study, human lung tissue samples we found was cells, whereas expression detectable benign tissues. Silencing strongly inhibited proliferation adenocarcinoma...
Schistosome infection persists for decades. Parasites are in close contact with host peripheral blood immune cells, yet little is known about the regulatory interactions between parasites and these cells. Here, we report that extracellular vesicles (EVs) released from Schistosoma japonicum taken up primarily by macrophages other cells their miRNA cargo transferred into recipient Uptake of S. EV miR-125b bantam miRNAs increased macrophage proliferation TNF-α production regulating...
Programmed DNA elimination is a developmentally regulated process leading to the reproducible loss of specific genomic sequences. occurs in unicellular ciliates and variety metazoans, including invertebrates vertebrates. In metazoa, typically somatic cells during early development, leaving germline genome intact. Reference genomes for metazoa that undergo are not available. Here, we generated reference sequences eliminating pig parasitic nematode Ascaris suum horse parasite Parascaris...
We present evidence that a subset of mRNAs in the human parasitic trematode Schistosoma mansoni contain an identical 36-nucleotide spliced leader (SL) sequence at their 5' termini. The SL is derived from 90-nucleotide nonpolyadenylylated RNA (SL RNA), presumably by trans-splicing. Neither nor share significant identity with previously described trans-spliced leaders and RNAs trypanosomatid protozoans or nematodes. However, several features, such as predicted secondary structure,...
Analogs of the mRNA cap are widely employed to study processes involved in metabolism as well being useful biotechnology and medicinal applications. Here we describe synthesis six dinucleotide analogs bearing a single phosphorothioate modification at either α, β, or γ position 5′,5′-triphosphate chain. Three them were also modified with methyl groups 2′- O 7-methylguanosine produce anti-reverse (ARCAs). Due presence stereogenic P centers moieties, each analog was obtained mixture two...
A new approach to the estimation of folate activity is described. The system may be used as a semi-automated method or fully automated using AutoAnalyzer Mecolab equipment. methods use chloramphenicol-resistant strain L. casei test organism, and this eliminates need for sterilization aseptic addition. systems are able handle extremely large numbers samples in relatively short time. Results similar those obtained by standard techniques.
Schistosomes, blood flukes, are an important global public health concern. Paired adult female schistosomes produce large numbers of eggs that primarily responsible for the disease pathology and critical dissemination. Consequently, understanding schistosome sexual maturation egg production may open novel perspectives intervening with these processes to prevent clinical symptoms interrupt life-cycle blood-flukes. microRNAs (miRNAs) key regulators many biological including development, cell...
Human ascariasis is a major neglected tropical disease caused by the nematode Ascaris lumbricoides . We report 296 megabase (Mb) reference-quality genome comprised of 17,902 protein-coding genes derived from single, representative worm. An additional 68 worms were collected 60 human hosts in Kenyan villages where pig husbandry rare. Notably, majority these (63/68) possessed mitochondrial genomes that clustered closer to parasite suum than A. Comparative phylogenomic analyses identified over...
Parasitic helminths (worms belonging to several metazoan phyla) cause considerable morbidity and mortality in humans. They are an important veterinary problem, they result significant economic losses animal grazing agriculture. Experimental studies on parasitic have been limited by a lack of parasite cell lines methods for molecular genetic analyses. We evaluated particle bombardment (biolistics) as strategy introduce express nucleic acids these multicellular parasites. By using embryos the...
An automated system is described for the microbiological assay of serum and red cell folate concentrations using a chloramphenicol-resistant strain L. casei. Both whole-blood haemolysates are assayed directly without previous deproteinization. Results by this method comparable to those obtained standard manual assays. Reproducibility within between assays excellent. Special advantages its simplicity operation, economy reagents, capacity dealing with large numbers specimens (80-100 per hour)....
The stability of the genome is paramount to organisms. However, diverse eukaryotes carry out programmed DNA elimination in which portions or entire chromsomes are lost early development during sex determination. During parasitic nematode, Ascaris suum, 13% eliminated. How different genomic segments reproducibly retained discarded unknown. Here, we show that centromeric histone CENP-A localization plays a key role this process. We chromosomes holocentric germline mitoses, with distributed...
Infection with different Leishmania spp. protozoa can lead to a variety of clinical syndromes associated in many cases inflammatory responses the skin. Although macrophages harbor majority parasites throughout chronic infection, neutrophils are first cells migrate site infection. Whether promote parasite clearance or exacerbate disease murine models varies depending on susceptible resistant status host. Based hypothesis that contribute systemic state humans symptomatic L. braziliensis we...