A5101458702- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Hepatitis B Virus Studies
- Weed Control and Herbicide Applications
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Lung Cancer Treatments and Mutations
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- GABA and Rice Research
- Insect Resistance and Genetics
- Plant Reproductive Biology
- Microbial Metabolic Engineering and Bioproduction
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Genomics and Phylogenetic Studies
- Allelopathy and phytotoxic interactions
- Plant Disease Resistance and Genetics
- Cancer Immunotherapy and Biomarkers
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
Huazhong Agricultural University
2015-2025
Shanghai Jiao Tong University
2025
Shanghai First People's Hospital
2025
Xiamen University
2009-2024
Zhongshan Hospital of Xiamen University
2009-2024
Tongji University
2014-2023
Shanghai Zhangjiang Laboratory
2023
Shanghai Pulmonary Hospital
2014-2023
Dalian Medical University
2019
First Affiliated Hospital of Dalian Medical University
2019
Artemisinin-based therapies are the only effective treatment for malaria, most devastating disease in human history. To meet growing demand artemisinin and make it accessible to poorest, an inexpensive rapidly scalable production platform is urgently needed. Here we have developed a new synthetic biology approach, combinatorial supertransformation of transplastomic recipient lines (COSTREL), applied introduce complete pathway artemisinic acid, precursor artemisinin, into high-biomass crop...
Abstract The growth in world population, climate change, and resource scarcity necessitate a sustainable increase crop productivity. Photosynthesis major crops is limited by the inefficiency of key CO 2 -fixing enzyme Rubisco, owing to its low carboxylation rate poor ability discriminate between O . In cyanobacteria proteobacteria, carboxysomes function as central organelles that elevate levels around encapsulated Rubisco enhance carboxylation. There growing interest engineering into...
Transgene expression from the plant's plastid genome represents a promising strategy in molecular farming because of plastid's potential to accumulate foreign proteins high levels and increased biosafety provided by maternal mode organelle inheritance. In this article, we explore transplastomic plants produce human immunodeficiency virus (HIV) antigens as components an acquired syndrome (AIDS) vaccine. It is shown that HIV p24 (the major target T-cell-mediated immune responses HIV-positive...
Abstract Calcium‐dependent protein kinases (CPKs) are serine/threonine that function in plant stress responses. Although CPKs recognized as key messengers signal transduction, the specific roles of and molecular mechanisms underlying their activity remain largely unknown. Here, we characterized OsCPK24, a cytosol‐localized calcium‐dependent kinase rice. OsCPK24 was universally highly expressed rice plants induced by cold treatment. Whereas knockdown exhibited increased sensitivity to...
As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized SWEET gene named OsSWEET5 through its overexpression rice. Heterologous expression assay indicated that encoded galactose yeast. OsSWEET5-overexpressing plants displayed the phenotypes of retardation precocious senescence at seedling stage. GC-MS analysis showed levels were largely altered leaves plants. Molecular revealed these might be due to transcriptional changes...
Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous with faster anticipated enhance crop photosynthesis and productivity. requirement chaperones for expression assembly has obstructed efficient production functional foreign in chloroplasts. Here, we report...
Most plastid genes are part of operons and expressed as polycistronic mRNAs. Many primary transcripts undergo post-transcriptional processing in monocistronic or oligocistronic units. At least some not translatable, endonucleolytic may therefore be a prerequisite for translation to occur. As the requirements intercistronic mRNA into stable transcript well understood, we have sought define minimum sequence elements that trigger thus capable generating translatable We describe here vivo...
Abstract Background As the final stage of leaf development, senescence may cause decline photosynthesis and gradual reduction carbon assimilation, which makes it a possible limiting factor for crop yield. NACs are plant-specific transcription factors some have been confirmed to play important roles in regulating senescence. Results In this study, we reported member NAC family named OsNAP whose expression is associated with senescence, investigated its preliminary function during process The...
Chloroplast transformation of the high-biomass tobacco variety Maryland Mammoth has been assessed as a production platform for human immunodeficiency virus type 1 (HIV-1) p24 antigen. offers prospect higher yields intact functional protein per unit floor area contained glasshouse time prior to flowering. Two different constructs, pZSJH1p24 (for insertion native cDNA between rbcL and accD genes) pZF5 chloroplast-codon-optimized gene trnfM trnG) were examined p24. Plants generated with...
Summary Plastid‐encoded genes are coordinately transcribed by the nucleus‐encoded RNA polymerase (NEP) and plastid‐encoded (PEP). Resulting primary transcripts frequently subject to editing cytidine‐to‐uridine conversions at specific sites. The physiological role of many events is largely unknown. Here, we have used CRISPR/Cas9 technique in rice knock out a member PLS‐DYW subfamily pentatricopeptide repeat (PPR) proteins. We found that OsPPR16 responsible for single event position 545...
A few reports have indicated that a single gene confers resistance to bacterial blight, sheath blight and rice blast. In this study, we identified novel disease mutant gene, methyl esterase-like (osmesl) in rice. Mutant with T-DNA insertion displayed significant caused by Xanthomonas oryzae pv. (Xoo), Rhizoctonia solani blast Magnaporthe oryzae. Additionally, CRISPR-Cas9 knockout mutants RNAi lines these pathogens. Complementary demonstrated phenotype similar the wild type (WT), thereby...
Summary Although Rubisco is the most abundant enzyme globally, it inefficient for carbon fixation because of its low turnover rate and limited ability to distinguish CO 2 O , especially under high conditions. To address these limitations, phytoplankton, including cyanobacteria algae, have evolved ‐concentrating mechanisms (CCM) that involve compartmentalizing within specific structures, such as carboxysomes in or pyrenoids algae. Engineering plant chloroplasts establish similar structures...
Summary Plants with transgenic plastid (chloroplast) genomes represent a promising production platform in molecular farming, mainly because of the plastids’ potential to accumulate foreign proteins very high levels and increased biosafety conferred by maternal mode inheritance. Although some transgenes can be expressed extraordinarily levels, expression others has been unsuccessful. Lack detectable transgene is usually attributable either RNA instability or protein instability. Here, we have...
High-level expression of foreign proteins in chloroplasts transplastomic plants provides excellent opportunities for the development oral vaccines against a range debilitating or fatal diseases. The HIV-1 capsid protein p24 and fusion with negative regulatory Nef (p24-Nef) accumulate to ∼4% ∼40% total soluble leaves tobacco (Nicotiana tabacum L.) plants. This study has investigated immunogenicity mice these two proteins, using cholera toxin B subunit as an adjuvant. Subcutaneous immunization...
Background The brown planthopper (BPH), Nilaparvata lugens (Stå;l), which belongs to Homopteran, Delphacidae, is one of the most serious and destructive pests rice. Feeding BPH with homologous dsRNA in vitro can lead death BPH, gives a valuable clue prevention control this pest, however, we know little about its small RNA world. Methodology/Principal Findings Small libraries for three developmental stages (CX-male adult, CC-female CY-last instar female nymph) had been constructed sequenced....
Agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. In this study, the vectors pMF-2A* containing a synthetic cry2A* gene driven by maize ubiquitin promoter and pCAMBIA1301 harboring hygromycin phosphotransferase (hpt) were introduced into Minghui86 (Oryza sativa L. ssp. indica), elite indica restorer line. Two independent transformants both hpt regenerated. Several homozygous progenies derived from family 2AH2, three of them selected...
As an important part of synthetic biology, promoter has gradually become a hotspot in current biology. The purposes the present study were to synthesize green tissue-specific promoters and discover cis-elements. We first assembled several regulatory sequences related expression different combinations, aiming obtain novel promoters. GUS assays transgenic plants indicated 5 showed patterns efficiencies various tissues. Subsequently, we scanned counted cis-elements based on plant database PLACE...
Abstract As glyphosate is a broad spectrum herbicide extensively used in agriculture worldwide, identification of new aroA genes with high level tolerance essential for the development and breeding transgenic glyphosate-tolerant crops. In this study, an gene was cloned from Janibacter sp. strain isolated marine sediment (designated as J. sp ). The purified enzyme has K m value 30 μM PEP 83 S3P significantly higher i (373 μM) than E. coli . AroA characterized novel naturally occurring class I...