A5062764928- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Stress Responses and Tolerance
- Biotin and Related Studies
- Plant Molecular Biology Research
- Plant responses to elevated CO2
- Algal biology and biofuel production
- Plant Gene Expression Analysis
- Botanical Research and Applications
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Biochemical and biochemical processes
- Microbial Metabolic Engineering and Bioproduction
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Autoimmune and Inflammatory Disorders Research
- Polysaccharides and Plant Cell Walls
- Plant and Biological Electrophysiology Studies
- Biofuel production and bioconversion
- Immune Cell Function and Interaction
- Metabolomics and Mass Spectrometry Studies
- Protist diversity and phylogeny
- Plant Water Relations and Carbon Dynamics
- Coffee research and impacts
University of Liverpool
2014-2024
Genomics (United Kingdom)
2020-2021
University College London
2020
University College Hospital
2020
University of Glasgow
1996-2005
University of Arizona
2005
University of York
2005
University of Nevada, Reno
2005
University of Manchester
1996
Abstract Crassulacean acid metabolism (CAM) is a water-use efficient adaptation of photosynthesis that has evolved independently many times in diverse lineages flowering plants. We hypothesize convergent evolution protein sequence and temporal gene expression underpins the independent emergences CAM from C 3 photosynthesis. To test this hypothesis, we generate de novo genome assembly genome-wide transcript data for Kalanchoë fedtschenkoi , an obligate species within core eudicots with...
Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous with faster anticipated enhance crop photosynthesis and productivity. requirement chaperones for expression assembly has obstructed efficient production functional foreign in chloroplasts. Here, we report...
Phosphorylation of phosphoenolpyruvate carboxylase plays a key role in the control plant metabolism. Phosphoenolpyruvate kinase is Ca2+-independent enzyme that activated by process involving protein synthesis response to range signals different tissues. The component whose required for activation has not previously been identified, nor characterised at molecular level. We report cloning from Crassulacean Acid Metabolism Kalanchoë fedtschenkoi and C3 Arabidopsis thaliana. Surprisingly, member...
The plant circadian clock plays an important role in enhancing performance and increasing vegetative yield. Much of our current understanding the mechanism function has come from development Arabidopsis thaliana as a model organism. Key to this rapid progress been robust markers, specifically circadian-regulated luciferase reporter genes. Studies crop species non-model organisms are currently hindered by absence simple high-throughput universal assay for function, accuracy robustness....
During the diversification of living organisms, novel adaptive traits usually evolve through co-option preexisting genes.However, most enzymes are encoded by gene families, whose members vary in their expression and catalytic properties.Each may therefore differ its suitability for recruitment into a function.In this work, we test presence such bias using example C 4 photosynthesis, complex trait that evolved recurrently flowering plants as response to atmospheric CO 2 depletion.We combined...
Mitochondrial NAD-malic enzyme (ME) and/or cytosolic/plastidic NADP-ME combined with the pyruvate orthophosphate dikinase (PPDK) catalyze two key steps during light-period malate decarboxylation that underpin secondary CO2 fixation in some Crassulacean acid metabolism (CAM) species. We report generation and phenotypic characterization of transgenic RNA interference lines obligate CAM species Kalanchoë fedtschenkoi reduced activities NAD-ME or PPDK. Transgenic line rNAD-ME1 had 8%, rPPDK1 5%...
Phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31) catalyzes primary nocturnal CO2 fixation in Crassulacean acid metabolism (CAM) species. CAM PPC is regulated posttranslationally by a circadian clock-controlled protein kinase called phosphoenolpyruvate (PPCK). PPCK phosphorylates during the dark period, reducing its sensitivity to feedback inhibition malate and thus enhancing stored malate. Here, we report generation characterization of transgenic RNAi lines obligate species Kalanchoë...
Unlike C3 plants, Crassulacean acid metabolism (CAM) plants fix CO2 in the dark using phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31). PPC combines with (as HCO3−), forming oxaloacetate. The oxaloacetate is converted to malate, leading malic accumulation vacuole, which peaks at dawn. During light period, malate decarboxylation concentrates around Rubisco for secondary fixation. CAM mutants lacking have not been described. Here, we employed RNA interference silence isogene PPC1 Kalanchoë...
Abstract Phosphoenolpyruvate carboxylase (PEPc) catalyzes the primary fixation of CO2 in Crassulacean acid metabolism plants. Flux through enzyme is regulated by reversible phosphorylation. PEPc kinase controlled changes level its translatable mRNA response to a circadian rhythm. The physiological significance levels PEPc-kinase-translatable and involvement metabolites control was investigated subjectingKalanchoë daigremontiana leaves anaerobic conditions at night modulate magnitude malate...
Summary Phosphoenolpyruvate carboxylase is regulated by reversible phosphorylation in response to light C 3 and 4 plants a circadian oscillator CAM plants. Increases phosphoenolpyruvate kinase activity require protein synthesis. This requirement has been analysed quantifying translatable mRNA for this using vitro translation of isolated RNA followed direct assay activity. In leaves the plant Bryophyllum (Kalanchoë) fedtschenkoi , normal diurnal conditions, was 20‐fold more abundant at night...
One of the best-characterized physiological rhythms in plants is circadian rhythm CO(2) metabolism Crassulacean acid (CAM) plants, which focus here. The central components plant clock have been studied detail only Arabidopsis (Arabidopsis thaliana). Full-length cDNAs obtained encoding orthologs CIRCADIAN CLOCK-ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION1 (TOC1), EARLY FLOWERING4 (ELF4), ZEITLUPE (ZTL), FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1), FLOWERING3...
The emission of isoprene from the biosphere to atmosphere has a profound effect on Earth's atmospheric system. Until now, it been assumed that primary short-term controls are photosynthetically active radiation and temperature. Here we show emissions tropical tree (oil palm, Elaeis guineensis) under strong circadian control, clock is potentially able gate light-induced emissions. These rhythms robustly temperature compensated with still control at 38 degrees C. This well beyond acknowledged...
Although biochemically related, C4 and crassulacean acid metabolism (CAM) systems are expected to be incompatible. However, Portulaca species, including P. oleracea, operate CAM within a single leaf, the mechanisms behind this unique photosynthetic arrangement remain largely unknown. Here, we employed RNA-seq identify candidate genes involved exclusively or shared by CAM, provided an in-depth characterization of their transcript abundance patterns during drought-induced transitions in...
Portulaca oleracea is a C4 herb capable of performing CAM under drought stress. It distributed worldwide and either considered polymorphic species or complex subspecies, due to its numerous morphological variations. We evaluated plasticity within P. genotypes since the complexity surrounding this may be reflected in intraspecific variations photosynthetic behavior. Eleven subspecies from distant geographical locations one cultivar were morphologically physiologically characterized....
Abstract We have already reported the cloning of one phosphoenolpyruvate carboxylase kinase gene from Arabidopsis thaliana (Hartwell et al . 1999, Plant Journal 20, 333–342), hereafter termed PPCK1 A second putative ( PPCK2 ) was identified in genome. The corresponding cDNA amplified flower tissue by reverse transcriptase (RT)‐polymerase chain reaction (PCR). This transcribed and translated vitro translation products possessed high activity, confirming identity gene. expression both genes...