A5014796492- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Biotin and Related Studies
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Plant Surface Properties and Treatments
- Plant Reproductive Biology
- Insect and Pesticide Research
- Biochemical and biochemical processes
- Plant and animal studies
- Mass Spectrometry Techniques and Applications
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Herbal Medicine Research Studies
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Electrochemical sensors and biosensors
- Microbial Natural Products and Biosynthesis
- Postharvest Quality and Shelf Life Management
- Molecular Junctions and Nanostructures
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
Iowa State University
2016-2025
Ames National Laboratory
2004-2018
U.S. National Science Foundation
2017
United States Department of Energy
2011-2017
Institute of Molecular Biology and Biophysics
2002-2011
National Center for Genome Resources
2011
Donald Danforth Plant Science Center
2011
University of Florida
2011
University of California, Davis
1983-2010
University of Iowa
2010
The Metabolomics Standards Initiative (MSI) has recently released documents describing minimum parameters for reporting metabolomics experiments, in order to validate metabolomic studies and facilitate data exchange. encompassed by MSI include the biological study design, sample preparation, acquisition, processing, analysis interpretation relative hypotheses being evaluated. Herein we exemplify how such metadata can be reported using a small case - metabolite profiling GC-TOF mass...
Acetyl-CoA provides organisms with the chemical flexibility to biosynthesize a plethora of natural products that constitute much structural and functional diversity in nature. Recent studies have characterized novel ATP-citrate lyase (ACL) cytosol Arabidopsis thaliana. In this study, we report use antisense RNA technology generate series lines range ACL activity. Plants even moderately reduced activity complex, bonsai phenotype, miniaturized organs, smaller cells, aberrant plastid...
Abstract Acetyl-coenzyme A (CoA) is used in the cytosol of plant cells for synthesis a diverse set phytochemicals including waxes, isoprenoids, stilbenes, and flavonoids. The source cytosolic acetyl-CoA unclear. We identified two Arabidopsis cDNAs that encode proteins similar to amino carboxy portions human ATP-citrate lyase (ACL). Coexpression these yeast (Saccharomyces cerevisiae) confers ACL activity, indicating both genes are required activity. heteromeric enzyme composed distinct...
The chemical industry is currently reliant on a historically inexpensive, petroleum-based carbon feedstock that generates small collection of platform chemicals from which highly efficient conversions lead to the manufacture large variety products. Recently, number factors have coalesced provide impetus explore alternative renewable sources carbon. Here we discuss potential impact shifting non-renewable sources. This change biological will an opportunity for research community contribute...
Abstract Background Acyl-acyl carrier protein thioesterases (acyl-ACP TEs) catalyze the hydrolysis of thioester bond that links acyl chain to sulfhydryl group phosphopantetheine prosthetic ACP. This reaction terminates elongation fatty acid biosynthesis, and in plant seeds it is biochemical determinant compositions storage lipids. Results To explore acyl-ACP TE diversity identify novel ACP-TEs, 31 TEs from wide-ranging phylogenetic sources were characterized ascertain their vivo activities...
The regulation of expression the genes encoding large subunit (LSU) and small (SSU) ribulose 1,5-bisphosphate carboxylase (RuBPCase) was examined in 1- through 8-day-old, dark-grown (etiolated) light-grown amaranth cotyledons. RuBPCase specific activity cotyledons increased during this 8-day period to a level 15-fold higher than Under both growth conditions, accumulation LSU SSU polypeptides not coordinated. Initial detection occurred 1 2 days after appearance light- cotyledons,...
Summary Laser desorption/ionization (LDI)‐based imaging mass spectrometry (MS) has been applied to several biological systems obtain information about both the identities of major chemical species and their localization. Colloidal graphite‐assisted LDI (GALDI) MS was introduced for small molecules such as phospholipids, cerebrosides, oligosaccharides, flavonoids, other secondary metabolites with high spatial homogeneity due finely dispersed particles. Mass profiles images Arabidopsis...
Abstract The ontogeny of seed structure and the accumulation storage substances is result a determinant genetic program. Using RNA interference, synthesis soybean (Glycine max) glycinin conglycinin proteins has been suppressed. protein knockdown (SP−) seeds are overtly identical to wild type, maturing similar size weight, in developmental ontogeny. SP− rebalance proteome, maintaining wild-type levels triglycerides. soybeans were evaluated with systems biology techniques proteomics,...
High-spatial resolution and high-mass techniques are developed adopted for the mass spectrometric imaging of epicuticular lipids on surface Arabidopsis thaliana. Single cell level spatial ∼12 μm was achieved by reducing laser beam size using an optical fiber with 25 core diameter in a vacuum matrix-assisted desorption ionization-linear ion trap (vMALDI-LTQ) spectrometer improved matrix application oscillating capillary nebulizer. Fine chemical images whole flower were visualized this high...
A 470-kb segment from the long arm of chromosome 3 Zea mays (inbred LH82), encompassing a1-sh2 interval, was cloned as a yeast artificial chromosome. Comparison sizes restriction fragments generated DNA fragment and isolated maize inbred line LH82 established colinearity interval in these DNAs. By utilizing fragmentation technique, separately fragmented at a1 sh2 loci. products physical distance between loci to be 140 kb. Furthermore, experiments orientation genes relative centromere. The...
Abstract Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is precursor for biosynthesis of fatty acids and, through them, a range important biomolecules. The source acetyl-CoA in not known, but two enzymes are thought to be involved: synthetase and plastidic pyruvate dehydrogenase. To determine importance these synthesizing during lipid accumulation developing Arabidopsis seeds, we isolated cDNA clones ptE1α- ptE1β-subunits our knowledge, this first reported sequence from plant...
Prunella vulgaris has been used therapeutically for inflammation-related conditions centuries, but systematic studies of its anti-inflammatory activity are lacking and no specific active components have identified. In this study, water ethanol extracts four P. accessions were applied to RAW 264.7 mouse macrophages, the significantly inhibited lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) nitric oxide (NO) production at 30 microg/mL without affecting cell viability. Extracts...
PlantMetabolomics.org (PM) is a web portal and database for exploring, visualizing, downloading plant metabolomics data. Widespread public access to well-annotated datasets essential establishing as functional genomics tool. PM integrates data generated from different analytical platforms multiple laboratories along with the key visualization tools such ratio error plots. Visualization can quickly show how one condition compares another which largest changes. The tries capture complete...