A5013623222- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Click Chemistry and Applications
- Essential Oils and Antimicrobial Activity
- Lipid metabolism and biosynthesis
- Drug-Induced Adverse Reactions
- Insect Resistance and Genetics
- SARS-CoV-2 and COVID-19 Research
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Pharmacological Effects of Natural Compounds
- Studies on Chitinases and Chitosanases
- Pharmacovigilance and Adverse Drug Reactions
- Enzyme Structure and Function
Iowa State University
2011-2024
Marlboro College
2022
Shanghai Jiao Tong University
2008-2011
Artemisinin is an effective anti-malarial drug isolated from Artemisia annua L. (Chinese wormwood), but the content of artemisinin in A. low. In present study we explored possibility using genetic engineering to increase by suppressing expression SQS (squalene synthase), a key enzyme sterol pathway (a competitive with that biosynthesis) means hairpin-RNA-mediated RNAi (RNA interference) technique. A total 23 independent transgenic plants were obtained through Agrobacterium...
Abstract Background Acyl-acyl carrier protein thioesterases (acyl-ACP TEs) catalyze the hydrolysis of thioester bond that links acyl chain to sulfhydryl group phosphopantetheine prosthetic ACP. This reaction terminates elongation fatty acid biosynthesis, and in plant seeds it is biochemical determinant compositions storage lipids. Results To explore acyl-ACP TE diversity identify novel ACP-TEs, 31 TEs from wide-ranging phylogenetic sources were characterized ascertain their vivo activities...
The substrate specificity of acyl-ACP thioesterase (TE) plays an essential role in controlling the fatty acid profile produced by type II synthases. Here we identify two groups residues that synergistically determine different specificities TEs from Cuphea viscosissima (CvFatB1 and CvFatB2). One group (V194, V217, N223, R226, R227, I268 CvFatB2) is critical determining structure depth a hydrophobic cavity N-terminal hotdog domain binds substrate's acyl moiety. other (255-RKLSKI-260...
Artemisinin is an effective anti-malarial drug extracted from Artemisia annua L. Due to the low content of artemisinin in A. annua, great efforts have been devoted improve production. Here we report a simple and efficient procedure enhance by seeding salinity stress. Our result shows that plant treated with 4 - 6 g/l NaCl could be significantly enhanced (up 2 3% dry weight) compared control (1% weight).
Artemisinin, a sesquiterpene lactone endoperoxide derived from the plant Artemisia annuaL., is considered to be most effective antimalarial drug. To increase production of artemisinin, the hmgr and fps genes which encode two key enzymes involved in artemisinin synthesis, were cloned from A. annua, respectively. The genes inserted between cauliflower mosaic virus 35S promoter and nopaline synthase terminator construct expression cassettes, then into plant vector pCAMBIA2300 obtain p2300-gfh...
Modulating the catalytic activity of acyl-ACP thioesterase (TE) is an important biotechnological target for effectively increasing flux and diversifying products fatty acid biosynthesis pathway. In this study, a directed evolution approach was developed to improve titer diversity produced by E. coli strains expressing variant TEs. A single round in vitro evolution, coupled with high-throughput colorimetric screen, identified 26 novel TE variants that convey up 10-fold increase titer,...
In plants and bacteria that use a Type II fatty acid synthase, isozymes of acyl-acyl carrier protein (ACP) thioesterase (TE) hydrolyze the thioester bond acyl-ACPs, terminating process biosynthesis. These TEs are therefore critical in determining profiles produced by these organisms. Past characterizations limited number plant-sourced acyl-ACP have suggested thiol-based, papain-like catalytic mechanism, involving triad Cys, His, Asn residues. present study, sequence alignment 1019 plant...
Little is known about cardiovascular safety profiles for monoclonal antibody products that received the FDA Emergency Use Authorization COVID-19. In this study, data from Adverse Event Reporting System first quarter of 2020 to second 2022 were used investigate signals associated with seven (casirivimab + imdevimab, bamlanivimab, bamlanivimab etesevimab, sotrovimab, tocilizumab, bebtelovimab, tixagevimab cilgavimab) in COVID-19 patients. Disproportionality analyses conducted using reporting...
Abstract Modulating the catalytic activity of acyl-ACP thioesterase (TE) is an important biotechnological target for effectively increasing flux and diversifying products fatty acid biosynthesis pathway. In this study, a directed evolution approach was developed to improve productivity diversity E. coli strains expressing variant TEs. A single round in vitro evolution, coupled with high-throughput colorimetric screen, identified 26 novel TE variants, which convey up 10-fold increase...