A5001190912- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Essential Oils and Antimicrobial Activity
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Biochemical and biochemical processes
- Plant Pathogens and Fungal Diseases
- Antioxidant Activity and Oxidative Stress
- Lipid metabolism and biosynthesis
- Natural product bioactivities and synthesis
- Plant Molecular Biology Research
- Phytochemicals and Antioxidant Activities
- Bioinformatics and Genomic Networks
- Plant tissue culture and regeneration
- Plant and animal studies
- Genomics and Phylogenetic Studies
- Analytical Chemistry and Chromatography
- Plant Parasitism and Resistance
- Horticultural and Viticultural Research
- Water Treatment and Disinfection
- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
Washington State University
2015-2024
IWW Water Centre
2002-2018
University of Tübingen
1998-2013
University of Bonn
2013
Grünenthal Group (Germany)
2013
Virginia Tech
2010
Carnegie Department of Plant Biology
2010
Burton Hospitals NHS Foundation Trust
2010
Noble Research Institute
2010
University of California, Davis
2010
Isopentenyl diphosphate (IPP) is the central intermediate in biosynthesis of isoprenoids, most ancient and diverse class natural products. Two distinct routes IPP occur nature: mevalonate pathway recently discovered deoxyxylulose 5-phosphate (DXP) pathway. The evolutionary history enzymes involved both phylogenetic distribution their genes across genomes suggest that germane to archaebacteria, DXP eubacteria, eukaryotes have inherited for from prokaryotes. occurrence specific restricted...
A systematic proteomic analysis of rice ( Oryza sativa ) leaf, root, and seed tissue using two independent technologies, two-dimensional gel electrophoresis followed by tandem mass spectrometry multidimensional protein identification technology, allowed the detection 2,528 unique proteins, which represents most comprehensive proteome exploration to date. comparative display expression patterns indicated that enzymes involved in central metabolic pathways are present all tissues, whereas...
In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in synthesis, such as opr3, are male-sterile but become fertile when applied to developing flower buds. We have used ATH1 oligonucleotide arrays follow gene expression opr3 stamens 22 h following treatment. these experiments, a total of 821 genes were specifically induced by 480 repressed. Comparisons with data from previous studies indicate that constitute stamen-specific transcriptome, large...
Isopentenyl diphosphate, the common precursor of all isoprenoids, has been widely assumed to be synthesized by acetate/mevalonate pathway in organisms. However, based on vivo feeding experiments, isopentenyl diphosphate formation several eubacteria, a green alga, and plant chloroplasts demonstrated very recently originate via mevalonate-independent route from pyruvate glyceraldehyde 3-phosphate as precursors. Here we describe cloning peppermint ( Mentha × piperita ) heterologous expression...
Functional genomics approaches, which use combined computational and expression-based analyses of large amounts sequence information, are emerging as powerful tools to accelerate the comprehensive understanding cellular metabolism in specialized tissues whole organisms. As part an ongoing effort identify genes essential oil (monoterpene) biosynthesis, we have obtained information from 1,316 randomly selected cDNA clones, or expressed tags (ESTs), a peppermint ( Mentha x piperita ) gland...
Suspension cultures of Picea abies (L.) Karst released polymeric material into the culture medium when treated with an elicitor preparation from spruce needle pathogen Rhizosphaera kalkhoffii. The presence lignin (about 35%, w/w) was demonstrated by phloroglucinol/HCI reactivity and quantitation thioglycolic acid. Carbohydrate 14%, protein 32%, were also detected. Amino acid analysis revealed that hydroxyproline proline predominated. Thioacidolysis subsequent Raney nickel desulfurization...
Peppermint ( Mentha × piperita L.) was transformed with various gene constructs to evaluate the utility of metabolic engineering for improving essential oil yield and composition. Oil increases were achieved by overexpressing genes involved in supply precursors through 2 C -methyl-D-erythritol 4-phosphate (MEP) pathway. Two-gene combinations enhance both composition a single transgenic line assessed as well. The most promising results obtained transforming plants expressing an antisense...
Glandular trichomes are specialized anatomical structures that accumulate secretions with important biological roles in plant-environment interactions. These also have commercial uses the flavor, fragrance, and pharmaceutical industries. The capitate-stalked glandular of Cannabis sativa (cannabis), situated on surfaces bracts female flowers, primary site for biosynthesis storage resins rich cannabinoids terpenoids. In this study, we profiled nine cannabis strains purportedly different...
The integration of mathematical modeling and experimental testing is emerging as a powerful approach for improving our understanding the regulation metabolic pathways. In this study, we report on development kinetic model that accurately simulates developmental patterns monoterpenoid essential oil accumulation in peppermint ( Mentha × piperita ). This was then used to evaluate biochemical processes underlying experimentally determined changes monoterpene pathway under low ambient-light...
Abstract Epithelial cells (ECs) lining the secretory cavities of Citrus peel have been hypothesized to be responsible for synthesis essential oil, but direct evidence such a role is currently sparse. We used laser-capture microdissection and pressure catapulting isolate ECs parenchyma (as controls not synthesizing oil) from young grapefruit (Citrus × paradisi ‘Duncan’), isolated RNA, evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis these data sets...
PlantMetabolomics.org (PM) is a web portal and database for exploring, visualizing, downloading plant metabolomics data. Widespread public access to well-annotated datasets essential establishing as functional genomics tool. PM integrates data generated from different analytical platforms multiple laboratories along with the key visualization tools such ratio error plots. Visualization can quickly show how one condition compares another which largest changes. The tries capture complete...