A5031052489- Plant Reproductive Biology
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic diversity and population structure
- Plant and animal studies
- Genetic and phenotypic traits in livestock
- Light effects on plants
- Bioenergy crop production and management
- Forest Insect Ecology and Management
- Greenhouse Technology and Climate Control
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Forest ecology and management
- Forest Ecology and Biodiversity Studies
- Species Distribution and Climate Change
- Genomics and Phylogenetic Studies
- Plant and Fungal Interactions Research
- Plant Water Relations and Carbon Dynamics
Johann Heinrich von Thünen-Institut
2019-2024
Institute of Genetics
2023
University of Copenhagen
2021
Max Planck Institute for Plant Breeding Research
2015-2020
Plant (United States)
2016
A dramatic evolution of fruit size has accompanied the domestication and improvement fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in genes CLAVATA-WUSCHEL signaling pathway have led to a significant increase generating enlarged meristems that lead flowers with extra organs bigger fruits. this work, by combining mapping-by-sequencing CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF FLORAL ORGANS (ENO), an AP2/ERF...
Abstract Poplar (Populus) is a well-established model system for tree genomics and molecular breeding, hybrid poplar widely used in forest plantations. However, distinguishing its diploid homologous chromosomes difficult, complicating advanced functional studies on specific alleles. In this study, we applied trio-binning design PacBio high-fidelity long-read sequencing to obtain haplotype-phased telomere-to-telomere genome assemblies the 2 parents of well-studied F1 “84K” (Populus alba ×...
Significance Internal timekeepers, called circadian clocks, are prevalent in all domains of life. Variation rhythms allows fine-tuning an organism to its specific environment. Here we show that a mutation LNK2 , addition the already described EID1 was responsible for deceleration cultivated tomatoes. We mutant alleles both genes arose earliest types and were selected during domestication process. Notably, specifically affect light input clock, leading light-conditional clock deceleration....
A well-established hypothesis for the evolution of dioecy involves two genes linked at a sex-determining region (SDR). Recently there has been increased interest in possible single gene sex determination. Work Populus finally provided direct experimental evidence determination plants using CRISPR-Cas9 to knock out and convert individuals from female male. In poplar, feminizing factor popARR17 acts as "master regulator", analogous mammalian masculinizing SRY. The production fully functional...
The number of dioecious species for which the genetic basis sex determination has been resolved is rapidly increasing. Nevertheless, molecular mechanisms downstream determinants remain largely elusive. Here, by RNA-sequencing early-flowering isogenic aspen ( Populus tremula ) lines differing exclusively switch gene ARR17 , we show that a narrowly defined network controls differential development female and male flowers. Although encodes type-A response regulator supposedly involved in...
RNA editing occurs in the endosymbiont organelles of higher plants as C-to-U conversions defined nucleotides. The availability large quantities sequencing data makes it possible to identify sites and quantify their extent. We have investigated 34 protein-coding mitochondrial transcripts four Populus species, a genus noteworthy for its remarkably small number compared other angiosperms. 27 these were subject at least one species. In total, 355 identified with high confidence, extents ranging...
Whole-genome sequencing of non-model organisms is now widely accessible and has allowed a range questions in the field molecular ecology to be investigated with greater power. However, some genomic regions that are high biological interest remain problematic for assembly data-handling. Three such major histocompatibility complex (MHC), sex-determining (SDRs) plant self-incompatibility locus (S-locus). Using these as examples, we illustrate challenges both assembling resequencing highly...
Advances in DNA sequencing technologies allow the of whole genomes thousands individuals and provide several million single nucleotide polymorphisms (SNPs) per individual. These data combined with precise high-throughput phenotyping enable genome-wide association studies (GWAS) identification SNPs underlying traits complex genetic architectures. The identified causal estimated allelic effects could then be used for advanced marker-assisted selection (MAS) breeding programs. But such MAS...
Under field conditions, drought and heat stress typically happen simultaneously their negative impact on the agricultural production is expected to increase worldwide under climate change scenario. In this study, we performed RNA-sequencing analysis leaves of wild barley (Hordeum spontaneum) originated from northern coastal region Egypt following individual acclimation (DA) shock (HS) treatments combination (CS, combined stresses) distinguish unique shared differentially expressed genes...
Nucleosome-depleted regions (NDRs) at gene promoters support initiation of RNA polymerase II transcription. Interestingly, transcription often initiates in both directions, resulting an mRNA and a divergent non-coding (DNC) transcript unclear purpose. Here, we characterized the genetic architecture molecular mechanism DNC budding yeast. Using high-throughput reverse screens based on quantitative single-cell fluorescence measurements, identified Hda1 histone deacetylase complex (Hda1C) as...
Abstract Many dioecious angiosperms are trees, which only flower after years of vegetative development and do not usually exhibit marked secondary sexual dimorphism. Nevertheless, if the genetic basis sex determination is known, an individual can be determined using molecular markers. Here, we report that in genus Populus sect. XY system determination, found P. tremula tremuloides , likely re-evolved from a ZW present alba, adenopoda qiongdaoensis . Strikingly, this new mechanistically...
Linnaeus's very first opus, written when he was 22 years old, dealt with the analogy that exists between plants and animals in how they 'propagate their species', a revised version plate depicting union of male female Mercurialis annua became foundational text on sexuality plants. The question systems separate males females have evolved sedentary organisms appear ancestrally bisexual has fascinated biologists ever since. phenomenon, termed dioecy, important consequences for plant...
Herbivorous insects can have a profound impact on plant growth performance. In some years, canopy damage in poplar plantations exceeds 50% of the total leaf surface, thereby possibly compromising carbon fixation and biomass yield. To assess transcriptional response elite clones to insect feeding test whether this varies between different genotypes, we performed an RNA-sequencing experiment. We deeply sequenced transcriptomes eight belonging three species (Populus trichocarpa, P. nigra...
Abstract Advances in DNA sequencing technologies allow the of whole genomes thousands individuals and provide several million single nucleotide polymorphisms (SNPs) per individual. These data combined with precise high-throughput phenotyping enable genome-wide association studies (GWAS) identification SNPs underlying traits complex genetic architectures. The identified causal estimated allelic effects could then be used for advanced marker-assisted selection (MAS) breeding programs. But such...
Abstract SNPscan breeder is a software that enables the simulation of breeding programs using simulated individual whole genome data, different genetic architectures trait interest, mating designs and selection criteria, i.e. pheno-types, values from progeny tests, marker-assisted (MAS) genomic (GS). The impact population size, design, intensity, architecture, heritability criteria on gains, kinship, inbreeding diversity can be evaluated to optimize program. A special feature possibility for...
Abstract Plant genome sequencing based on long reads has increasingly been applied also to tree species in recent years. A crucial step these projects is the successful extraction of high-molecular-weight DNA high quality and sufficient quantity, which imperative for long-read sequencing. The trees limited difficult conduct. To achieve extraction, modification protocol necessary. Here, we present a from broadleaved Fraxinus excelsior conifer Taxus baccata . nuclei isolation divided into two...