A5040770479- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Microbial Community Ecology and Physiology
- Advanced Proteomics Techniques and Applications
- Ginseng Biological Effects and Applications
- Plant biochemistry and biosynthesis
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Bacteriophages and microbial interactions
- Marine and coastal ecosystems
- Neuropeptides and Animal Physiology
- Plant Pathogenic Bacteria Studies
- Diet and metabolism studies
- Gut microbiota and health
- Genetic Associations and Epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Computational Drug Discovery Methods
- Tree Root and Stability Studies
- Genomics and Phylogenetic Studies
- Receptor Mechanisms and Signaling
- Species Distribution and Climate Change
- Postharvest Quality and Shelf Life Management
- Plant Surface Properties and Treatments
- Plant Gene Expression Analysis
- Lipid metabolism and biosynthesis
Weizmann Institute of Science
2010-2024
Al-Qasemi Academic College of Education
2016
Zero to Three
2016
Hebrew University of Jerusalem
2013-2014
Fondazione Edmund Mach
2013-2014
University of Haifa
2010
Annotation of metabolites is an essential, yet problematic, aspect mass spectrometry (MS)-based metabolomics assays. The current repertoire definitive annotations metabolite spectra in public MS databases limited and suffers from lack chemical taxonomic diversity. Furthermore, the heterogeneity data prevents development universally applicable annotation tools. Here we present a combined experimental computational platform to advance this key issue metabolomics. WEIZMASS unique reference...
Mapping the exometabolic landscape during E. huxleyi bloom succession reveals a halogenated footprint of viral infection.
The output of LC-MS metabolomics experiments consists mass-peak intensities identified through a peak-picking/alignment procedure. Besides imperfections in biological samples and instrumentation, data accuracy is highly dependent on the applied algorithms their parameters. Consequently, quality control (QC) essential for further analysis. Here, we present QC approach that based discrepancies between replicate samples. First, quantile normalization per-sample log-signal distributions to each...
Due to their sensitivity and speed, mass-spectrometry based analytical technologies are widely used in metabolomics characterize biological phenomena. To address issues like metadata organization, quality assessment, data processing, storage, and, finally, submission public repositories, bioinformatic pipelines of a non-interactive nature often employed, complementing the interactive software for initial inspection visualization data. These created as open-source allowing complete exhaustive...
Estimation of mass measurement accuracy is an elementary step in the application spectroscopy (MS) data towards metabolite annotations and has been addressed several times past. However, reproducibility measurements over a diverse set analytes variable operating conditions, which are common high-throughput metabolomics studies, has, to best our knowledge, not so far.A method automatically extract errors from large made on quadrupole time-of-flight (QTOF) MS instrument developed. The size...
Suberized and/or lignified (i.e. lignosuberized) periderm tissue appears often on surface of fleshy fruit skin by mechanical damage caused following environmental cues or developmental programs. The mechanisms underlying lignosuberization remain largely unknown to date. Here, we combined an assortment microscopical techniques with integrative multi-omics approach comprising proteomics, metabolomics and lipidomics identify novel molecular components involved in lignosuberization. We chose...
G protein-coupled receptors (GPCRs) are a super-family of membrane proteins that attract great pharmaceutical interest due to their involvement in almost every physiological activity, including extracellular stimuli, neurotransmission, and hormone regulation. Currently, structural information on many GPCRs is mainly obtained by the techniques computer modelling general homology particular. Based quantitative analysis eighteen antagonist-bound, resolved structures rhodopsin family "A" - also...
Abstract Tapping into the metabolic cross-talk between a host and its virus can reveal unique strategies employed during infection. Viral infection is dynamic process that generates an evolving landscape. Gaining continuous view highly challenging limited by current metabolomics approaches, which typically measure average of entire population at various stages Here, we took novel approach to study basis host-virus interactions bloom-forming alga Emiliania huxleyi specific virus. We combined...
Abstract Algal blooms are important hotspots of primary production in the ocean, forming basis marine food web and fueling pool dissolved organic matter (DOM) 1 , which is largest global inventory reduced carbon a market place for metabolic exchange ocean 2 . Marine viruses key players controlling algal bloom demise act as major biogeochemical drivers nutrient cycling fluxes by shunting biomass from higher trophic levels to DOM pool, process termed ‘viral shunt’ 3,4 Nevertheless, composition...
Brassica rapa (B. rapa) and its subspecies contain many bioactive metabolites that are important for plant defense human health. This study aimed at investigating the metabolite composition variation among a large collection of B. genotypes, including their accessions. Metabolite profiling leaves 102 genotypes was performed using ultra-performance liquid chromatography coupled with photodiode array detector quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS). In total, 346...