A5013536070- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Electrolyte and hormonal disorders
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Monoclonal and Polyclonal Antibodies Research
- Chemical Reaction Mechanisms
- Pharmacological Receptor Mechanisms and Effects
- Peptidase Inhibition and Analysis
- Anesthesia and Neurotoxicity Research
- Chemical Synthesis and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nitric Oxide and Endothelin Effects
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
- Nicotinic Acetylcholine Receptors Study
- Protein Kinase Regulation and GTPase Signaling
- Chemotherapy-induced cardiotoxicity and mitigation
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Mass Spectrometry Techniques and Applications
- Marine Toxins and Detection Methods
University of Birmingham
2013-2024
University of Nottingham
1984-2024
Coventry University
2019-2024
Receptors (United States)
2019
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
2018-2019
University of Southampton
2010
University of Warwick
2008
Aston University
2006-2008
University of Auckland
2008
Swansea University
2008
<h3>Background</h3> Clinicians use fibrosis in a liver biopsy to predict clinical outcomes of chronic disease. The performance non-invasive tests has been evaluated against histological assessment but as the reference standard would be ideal. enhanced (ELF) test was derived and validated large cohort patients shown have high diagnostic accuracy (area under curve (AUC)=0.80 95% CI 0.76 0.85) identification significant on biopsy. <h3>Objective</h3> To evaluate ELF predicting by following up...
G-protein coupled receptors (GPCRs) constitute the largest class of membrane proteins and are a major drug target. A serious obstacle to studying GPCR structure/function characteristics is requirement extract from their native environment in plasma membrane, with inherent instability GPCRs detergents required for solubilization. In present study, we report first solubilization purification functional [human adenosine A2A receptor (A2AR)], total absence detergent at any stage, by exploiting...
Assessment of liver fibrosis is important in determining prognosis and evaluating interventions. Due to limitations accuracy patient hazard biopsy, non-invasive methods have been sought provide information on fibrosis, including the European (ELF) test, shown good diagnostic for detection moderate severe fibrosis. Access independent cohorts patients has provided an opportunity explore if this test could be simplified. This paper reports simplification ELF its ability identity severity...
Positively charged poly(styrene-<italic>co</italic>-maleimide) extracts functional membrane proteins into nanodiscs, overcoming some limitations of current nanodisc technology.
Abstract Muscarinic acetylcholine receptors were purified from rat forebrain and labeled with [3H]N-(2-chloroethyl)N-(2',3'-[3H2]propyl)-2-aminoethylbenzilate. Cleavage of the muscarinic a lysine-specific protease yielded labeled, glycosylated peptides about 130 200 residues in length, which came different receptor sequences. The probable cleavage sites are second intracellular loop extracellular or third loop. N-terminal disulfide-bonded to another part structure, supporting presence link...
Combining the technologies of encapsulation GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.
In this study, we establish that the V1a vasopressin receptor (V1aR) is palmitoylated, and show modification has an important functional role. Palmitoylation of V1aR occurs within Cys371/Cys372 couplet located in proximal C-terminal tail domain. Substitution these residues a [C371G/C372G]V1aR construct effectively disrupted palmitoylation. Our data also indicate additional palmitoylation site at another locus receptor, as yet undefined. [3H]Palmitate incorporation was agonist-sensitive...
The [Arg8]vasopressin (AVP) receptor expressed by human hepatocytes was characterized, and compared with the rat hepatic V1a vasopressin subtype. In addition to determining pharmacological profile of receptor, cellular responses AVP were measured in assaying glycogen phosphorylase alpha activity DNA synthesis. Marked differences observed between regarding receptors intracellular consequences stimulation AVP. Data presented this paper demonstrate following, (i) Vasopressin are present low...
Calcitonin receptor like-receptor is a family B G-protein coupled (GPCR). It requires activity modifying protein (RAMP) 1 to give calcitonin gene-related peptide (CGRP) receptor. Little known of how members this function. Proline residues often form important kinks in α-helices. Therefore, all proline within the transmembrane helices (Pro241, Pro244 helix 4, Pro275 5, Pro321 and Pro331 6) were mutated alanine. Pro241, Pro275, are highly conserved throughout GPCRs. The binding CGRP its...
Although the ability of hormones and growth factors to stimulate DNA synthesis in rat hepatocytes has been investigated extensively, no such study human reported. Here we describe a series experiments identify those that regulate hepatocyte vitro which therefore may play role control liver regeneration. Human were isolated from normal tissue obtained after graft reduction for transplantation into pediatric recipients. Cells maintained culture up 5 days, was determined. Hydroxyurea reduced...
The adenosine 2A receptor (A2AR), a G-protein-coupled (GPCR), was solubilised and purified encapsulated in styrene maleic acid lipid particles (SMALPs). A2AR-SMALP associated with phospholipids characteristic of the plasma membrane Pichia pastoris, host used for its expression, confirming that native lipids. fluorescence spectrum showed broad emission peak at 330 nm, produced by endogenous Trp residues. inverse agonist ZM241385 caused 30% increase emission, unusually accompanied red-shift...
For G-protein-coupled receptors (GPCRs) in general, the roles of extracellular residues are not well defined compared with transmembrane helices (TMs). Nevertheless, important for various functions both peptide-GPCRs and amine-GPCRs. In this study, V(1a) vasopressin receptor was used to systematically investigate role charged that highly conserved throughout a subfamily peptide-GPCRs, using combination mutagenesis molecular modeling. Of 13 identified loops (ECLs), Arg(116) (ECL1), Arg(125)...