A5058816530- Ion channel regulation and function
- Protein Kinase Regulation and GTPase Signaling
- Calcium signaling and nucleotide metabolism
- Neuroscience and Neuropharmacology Research
- Ion Transport and Channel Regulation
- Toxic Organic Pollutants Impact
- Pancreatic function and diabetes
- Carcinogens and Genotoxicity Assessment
- Cellular transport and secretion
- Effects and risks of endocrine disrupting chemicals
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Trace Elements in Health
- Lipid Membrane Structure and Behavior
- Autophagy in Disease and Therapy
- Cardiac electrophysiology and arrhythmias
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Magnesium in Health and Disease
- Reproductive Biology and Fertility
- Bioactive natural compounds
- Adenosine and Purinergic Signaling
- Curcumin's Biomedical Applications
University of Chester
2015-2025
University of Birmingham
2009-2024
Instituto Venezolano de Investigaciones Científicas
2014
State Key Laboratory of Synthetic Chemistry
2010
Chinese University of Hong Kong
2010
University of Minnesota
2009
In-Q-Tel
2008
University of Padua
1991
University of Southampton
1988-1991
Autophagy is an important cellular process that controls cells in a normal homeostatic state by recycling nutrients to maintain energy levels for cell survival via the turnover of proteins and damaged organelles. However, persistent activation autophagy can lead excessive depletion organelles essential proteins, leading caspase-independent autophagic death. As such, inducing death through this mechanism could be alternative approach treatment cancers. Recently, we have identified novel...
Abstract Emerging evidence suggests that autophagic modulators have therapeutic potential. This study aims to identify novel inducers from traditional Chinese medicinal herbs as potential antitumor agents. Using an image-based screen and bioactivity-guided purification, we identified alisol B 23-acetate, A 24-acetate, the rhizome of Alisma orientale autophagy, with being most potent natural product. Across several cancer cell lines, showed B–treated cells displayed increase flux formation...
Early reports indicated that ECV304 was a spontaneously-transformed line derived from Japanese human umbilical vein endothelial cells (HUVEC) culture. Many morphological, immunochemical, and genetic studies provided further evidence valuable biomedical research tool could be used to study processes include angiogenesis in vitro signal transduction by variety of G protein-coupled receptors. However, several distinct differences between HUVEC are now apparent recent have similarity T24/83,...
Brominated flame retardants (BFRs) are chemicals commonly used to reduce the flammability of consumer products and considered pollutants since they have become widely dispersed throughout environment also been shown bio-accumulate within animals man. This study investigated cytotoxicity some most groups BFRs on SH-SY5Y human neuroblastoma cells. The results showed that tested, hexabromocyclododecane (HBCD), tetrabromobisphenol-A (TBBPA) decabromodiphenyl ether (DBPE), all cytotoxic at low...
Celastrol exhibits anti-arthritic effects in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca
2‐Aminoethoxydiphenyl Borate (2‐APB) has been extensively used recently as a membrane permeable modulator of inositol‐1,4,5‐trisphosphate‐sensitive Ca 2+ channels and store‐operated entry. Here, we report that 2‐APB is also an inhibitor sarco/endoplasmic reticulum ‐ATPase (SERCA) pumps, additionally increases ion leakage across the phospholipid bilayer. Therefore, advise caution in interpretation results when signalling experiments. The inhibition onthe SERCA pumps isoform‐dependent, with 2B...
2,5‐Di( tert ‐butyl)‐1,4‐benzohydroquinone has been shown to inhibit the Ca 2+ , Mg ‐ATPase of sarcoplasmic reticulum with an affinity 0.4 μM. It shift E2‐E1 equilibrium for ATPase towards E2, as previously inhibitor thapsigargin. The E2 results in a decrease also observed A marked rate transition is both BHQ and level phosphorylation by P i steady‐state lever ATP are consistent constant increase dephosphorylation.
Wolfram syndrome is an autosomal recessive disorder characterized by neurodegeneration and diabetes mellitus. The gene responsible for the (WFS1) encodes endoplasmic reticulum (ER)-resident transmembrane protein that involved in regulation of unfolded response (UPR), intracellular ion homeostasis, cyclic adenosine monophosphate production insulin biosynthesis secretion. In this study, single cell Ca2+ imaging with fura-2 direct measurements free cytosolic ATP concentration ([ATP]CYT)...
Abstract Resistance of cancer cells to chemotherapy is a significant clinical concern and mechanisms regulating cell death in therapy, including apoptosis, autophagy or necrosis, have been extensively investigated over the last decade. Accordingly, identification medicinal compounds against chemoresistant via new mechanism action highly desired. Autophagy important inducing survival therapy. Recently, novel activators isolated from natural products were shown induce autophagic...
Drug resistance in cancer has been classified as innate or acquired resistance, which were characterized by apoptotic defects and ABC transporters overexpression respectively. Therefore, to preclude reverse these mechanisms could be a promising strategy improve chemotherapeutic outcomes. In this study, natural product from Osage Orange, pomiferin, was identified novel autophagy activator that circumvents triggering autophagic cell death via SERCA inhibition activation of the CaMKKβ-AMPK-mTOR...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMembrane solubilization by detergent: use of brominated phospholipids to evaluate the detergent-induced changes in calcium-ATPase/lipid interactionBeatrice De Foresta, Marc Le Maire, Stephane Orlowski, Philippe Champeil, Sten Lund, Jesper V. Moeller, Francesco Michelangeli, and Anthony G. LeeCite this: Biochemistry 1989, 28, 6, 2558–2567Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of inhibition the calcium-magnesium ATPase by nonylphenolF. Michelangeli, S. Orlowski, P. Champeil, J. M. East, and A. G. LeeCite this: Biochemistry 1990, 29, 12, 3091–3101Publication Date (Print):March 1, 1990Publication History Published online1 May 2002Published inissue 1 March 1990https://pubs.acs.org/doi/10.1021/bi00464a028https://doi.org/10.1021/bi00464a028research-articleACS PublicationsRequest reuse permissionsArticle...
The sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitors thapsigargin (0.1-1 μM) and cyclopiazonic acid (10 μM), failed to affect resting [Ca2+] in human spermatozoa. Slow progesterone-induced [Ca2+ i]i oscillations spermatozoa, which involve cyclic emptying-refilling of an intracellular Ca2+ store were also insensitive these inhibitors. Non-selective doses (5-30 μM, 50-300 times the saturating dose for SERCA inhibition), caused elevation [Ca2+]i partial, dose-dependent...
Three isoforms of the sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase (SERCA) are known to exist in mammalian cells. This study investigated effects thapsigargin and a variety commonly used hydrophobic inhibitors on these SERCA (i.e. SERCA1b, SERCA2b, SERCA3a), which were transiently expressed COS-7 In addition, assessed whether introduction phenylalanine valine mutation at position 256 (F256V), reduce potency inhibition avian SERCA1, affects other similar manner this also by inhibitors....
Abstract Calcium is a second messenger which required for regulation of many cellular processes. However, excessive elevation or prolonged activation calcium signaling would lead to cell death. As such, selectively regulating could be an alternative approach anti-cancer therapy. Recently, we have identified effective analogue resveratrol, (Z)3,4,5,4′-trans-tetramethoxystilbene (TMS) elevated the intracellular level in gefitinib-resistant (G-R) non-small-cell lung cancer (NSCLC) cells. TMS...
Caenorhabditis elegans (C. elegans) tetraspanin-7 (TSP-7) protein is an orthologue of the Human tetraspanin CD63, which has recently been shown to be a negative regulator autophagy. In this study mutant strain wild-type (WT) C. (tm5761) with 352 bp deletion in tsp-7 gene, was studied. A polyclonal antibody raised peptide sequence present only (N2). This cross-reacted correct molecular weight (MW) WT lysate, but not tm5761, confirming absence functional TSP-7 strain. From life-span studies,...