A5090666610- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Pregnancy-related medical research
- Pregnancy and preeclampsia studies
- Systemic Lupus Erythematosus Research
- Protein Kinase Regulation and GTPase Signaling
- Food Allergy and Anaphylaxis Research
- Cellular transport and secretion
- Asthma and respiratory diseases
- Rheumatoid Arthritis Research and Therapies
- Carcinogens and Genotoxicity Assessment
- Allergic Rhinitis and Sensitization
- Genetically Modified Organisms Research
- Peripheral Neuropathies and Disorders
- Effects and risks of endocrine disrupting chemicals
- Coccidia and coccidiosis research
- Signaling Pathways in Disease
- Calcium signaling and nucleotide metabolism
- Monoclonal and Polyclonal Antibodies Research
- Parasitic Infections and Diagnostics
- Pregnancy and Medication Impact
- Neonatal Respiratory Health Research
- Parasite Biology and Host Interactions
- Endoplasmic Reticulum Stress and Disease
- Parasites and Host Interactions
Imperial College London
1980-2023
St George's, University of London
2014-2022
Chelsea and Westminster Hospital
2016-2019
Beijing Hospital
2015
University of Birmingham
2008-2012
The process of parturition involves the transformation quiescent myometrium (uterine smooth muscle) to highly contractile laboring state. This is thought be driven by changes in gene expression myometrial cells. Despite existence multiple studies, transcriptional programs that initiate labor are not known. Here, we integrated three transcriptome datasets, one novel (NCBI Gene Expression Ominibus: GSE80172) and two existing, characterize associated with onset at term. Computational analyses...
Blocking the bioactivity of allergens is conceptually attractive as a small-molecule therapy for allergic diseases but has not been attempted previously. Group 1 house dust mites (HDM) are meaningful targets in this quest because they globally prevalent and clinically important triggers asthma. HDM cysteine peptidases whose proteolytic activity essential steps allergy cascade. Using allergen Der p an archetype structure-based drug discovery, we have identified series novel, reversible...
Key points Over 15 million babies are born prematurely each year with approximately 1 of these dying as a direct result preterm delivery. β 2 ‐Adrenoreceptor agonists that act via cAMP can reduce uterine contractions to delay labour, but their ability repress lasts ≤ 48 h and use does not improve neonatal outcomes. Previous research has suggested inhibits myometrial protein kinase A (PKA) activation, this yet be demonstrated PKA‐specific agonists. We investigated the role PKA in mediating...
Objective. To evaluate the impact of RA on work capacity and identify factors related to impairment in patients with RA.
Myometrial explants represent a superior model compared with cell culture models for the study of human myometrial progesterone (P4) signalling in parturition. Gene expression analysis showed closely resemble vivo condition and anti-inflammatory action P4 is not lost labour onset. Circulating levels decline before onset parturition most animals, but humans. This has led to suggestion that there functional withdrawal at level prior However, date, no evidence loss function been provided,...
Regucalcin (RGN), also reported as senescence marker protein-30 (SMP30), plays a role in Ca2+ homeostasis by modulating number of Ca2+-dependent proteins. RGN cyto-protective and its decrease is linked to age-related diseases cell death. This study shows that reduces agonist (histamine)-induced transients RGN+ transfected COS-7 cells (RGN+) increases their storage capacity. These observations are explained having increased mRNA protein expression levels sarco/endoplasmic reticulum...
Abstract Although progesterone (P4) supplementation is the most widely used therapy for prevention of preterm labor (PTL), reports its clinical efficacy have been conflicting. We previously shown that anti-inflammatory effects P4 can be enhanced by increasing intracellular cyclic adenosine monophosphate (cAMP) levels in primary human myometrial cells. Here, we examined whether adding aminophylline (Am), a non-specific phosphodiesterase inhibitor increases cAMP levels, to might improve using...
Diabetes mellitus-related vascular disease is often associated with both a dysregulation of Ca2+ homoeostasis and enhanced secretory activity in VSMCs (vascular smooth muscle cells). Here, we employ commonly used rat cell line for (A7r5 cells) to investigate the effects glucose on expression SPCA1 (secretory pathway Ca2+-ATPase 1; also known as ATP2C1), which P-type pump located Golgi apparatus that plays key role pathway. Our results show mRNA levels are significantly increased A7r5 cells...
Nosema algerae and N. eurytremae were successfully cultured in embryonic rat brain, Xenopus XTC-6 Chang liver cells, brain cells respectively. No parasites grew cultures incubated at 38 °C but development took place 34 27 °C. Increased levels of infections the achieved by centrifugation spores on to cells. The level infection was also related type medium used for hatching spores, modifies NMRI giving better results than Leibowitz L15, interval between adding cell centrifugation.
Abstract Progesterone (P4) and cyclic adenosine monophosphate (cAMP) are regarded as pro‐quiescent factors that suppress uterine contractions during pregnancy. We previously used human primary cells in vitro mice vivo to demonstrate simultaneously enhancing myometrial P4 cAMP levels may reduce inflammation‐associated preterm labor. Here, we assessed whether aminophylline (Ami; phosphodiesterase inhibitor) can contractility contraction‐associated proteins (CAPs) better together than...
Whereas treatment of allergic diseases such as asthma relies largely on the targeting dysregulated effector pathways, conceptually attractive alternative preventing them by a pharmaceutical, at-source intervention has been stymied until now uncertainties about suitable targets and challenges facing drug design. House dust mites (HDMs) are globally significant triggers allergy. Group 1 HDM allergens, exemplified Der p 1, cysteine proteases. Their degradome strong disease linkage that...
During pregnancy, interactions between uterine immune cells and of the surrounding reproductive tissues are thought to be vital for regulating labour. The mechanism that specifically initiates spontaneous labour has not been determined, but distinct changes in cell populations their activation status have observed during at term gestation. To understand regulation human by system, ability isolate both non-immune from uterus is required. Here, we describe protocols developed our laboratory...
High throughput sequencing has previously identified differentially expressed genes (DEGs) and enriched signalling networks in human myometrium for term (≥37 weeks) gestation labour, when defined as a singular state of activity at comparison to the non-labouring state. However, transcriptome changes that occur during transition from early established labour (defined ≤3 >3 cm cervical dilatation, respectively) potentially altered by fetal membrane rupture (ROM), adapting onset completion...
<h3></h3> Preterm labour is the most significant cause of perinatal morbidity and mortality, causing socioeconomic consequences for individuals, families society. Our previous work has demonstrated that cAMP enhances ability progesterone to repress IL1B driven COX2 expression in myometrium. The fetal membranes, specifically amnion, have a potentially role initiation labour. Labour being pro-inflammatory state, could be delayed with modulation this inflammatory process. We treatment primary...