A5049448786- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Asthma and respiratory diseases
- Antimicrobial Peptides and Activities
- Web and Library Services
- Pharmaceutical studies and practices
- Cellular transport and secretion
- Clinical Laboratory Practices and Quality Control
- Carbohydrate Chemistry and Synthesis
- Oral microbiology and periodontitis research
- Peptidase Inhibition and Analysis
- Chemical Synthesis and Analysis
- Mobile Learning in Education
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Atomic and Subatomic Physics Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA regulation and disease
- Advanced Database Systems and Queries
- Click Chemistry and Applications
- Probiotics and Fermented Foods
- Gut microbiota and health
- Allergic Rhinitis and Sensitization
- Spectroscopy and Laser Applications
- Library Science and Information Systems
King's College London
2015-2024
Cumbria Northumberland Tyne and Wear NHS Foundation Trust
2023
Centre for Cellular and Molecular Biology
2022
The London College
2022
St George's, University of London
2014-2022
Good Things Foundation
2021
King's College - North Carolina
2017
University of Manchester
2004-2016
Babraham Institute
2016
University of Exeter
2016
Candidalysin, a hypha-associated fungal peptide, drives interleukin-17 responses to Candida albicans .
ABSTRACT Life-threatening systemic infections often occur due to the translocation of pathogens across gut barrier and into bloodstream. While microbial host mechanisms permitting bacterial are well characterized, these still unclear for fungal such as Candida albicans , a leading cause nosocomial bloodstream infections. In this study, we dissected cellular C. intestinal epithelia in vitro identified genes associated with process. We show that is dynamic process initiated by invasion...
ABSTRACT Unlike other forms of candidiasis, vulvovaginal caused primarily by the fungal pathogen Candida albicans , is a disease immunocompetent and otherwise healthy women. Despite its prevalence, factors responsible for initiating symptomatic infection remain poorly understood. One hallmarks vaginal candidiasis robust recruitment neutrophils to site infection, which seemingly do not clear fungus, but rather exacerbate symptomatology. Candidalysin, newly discovered peptide toxin secreted C....
Candida albicans is a fungal pathobiont, able to cause epithelial cell damage and immune activation. These functions have been attributed its secreted toxin, candidalysin, though the molecular mechanisms are poorly understood. Here, we identify epidermal growth factor receptor (EGFR) as critical component of candidalysin-triggered responses. We find that both C. candidalysin activate human EGFR receptors candidalysin-deficient mutants induce phosphorylation during murine oropharyngeal...
Candidalysin is the first cytolytic peptide toxin identified in any human fungal pathogen. secreted by Candida albicans and critical for driving infection host immune responses several model systems. However,
The fungal pathogen Candida albicans is linked to chronic brain diseases such as Alzheimer's disease (AD), but the molecular basis of anti-Candida immunity remains unknown. We show that C. enters mouse from blood and induces two neuroimmune sensing mechanisms involving secreted aspartic proteinases (Saps) candidalysin. Saps disrupt tight junction proteins blood-brain barrier (BBB) permit invasion. also hydrolyze amyloid precursor protein (APP) into β (Aβ)-like peptides bind Toll-like...
Peptides and proteins fragment sequence-specifically in the presence of 3-mercaptopropionic acid to afford thioesters which can be used native chemical ligation reactions.
Background. The ability of epithelial cells (ECs) to discriminate between commensal and pathogenic microbes is essential for healthy living. Key these interactions are mucosal responses pathogen-induced damage.
Protection against microbial infection by the induction of inflammation is a key function IL-1 superfamily, including both classical and new IL-36 cytokine families. Candida albicans frequent human fungal pathogen causing mucosal infections. Although initiators effectors important in protective host responses to C. are well described, players driving these remain poorly defined. Recent work has identified central role played inducing innate Type-17 immune clear Despite this, lack signaling...
Candida albicans is an opportunistic fungal pathogen responsible for superficial and life-threatening infections in humans. During mucosal infection, C. undergoes a morphological transition from yeast to invasive filamentous hyphae that secrete candidalysin, 31-amino-acid peptide toxin required virulence. Candidalysin damages epithelial cell plasma membranes stimulates the activating protein 1 (AP-1) transcription factor c-Fos (via p38-mitogen-activated kinase [MAPK]), MAPK phosphatase MKP1...
Vulvovaginal candidiasis (VVC), caused primarily by the human fungal pathogen Candida albicans , results in significant quality-of-life issues for women worldwide. Candidalysin, a toxin derived from polypeptide (Ece1p) encoded ECE1 gene, plays crucial role driving immunopathology at vaginal mucosa. This study aimed to determine if expression and/or processing of Ece1p differs across C . isolates and whether this partly underlies differential pathogenicity observed clinically. Using targeted...
The objective of entity identification is to determine the correspondence between object instances from more than one database. Entity at instance level, assuming that schema level heterogeneity has been resolved a priori, examined. Soundness and completeness are defined as desired properties any technique. To achieve soundness, set identity distinctness rules established for entities in integrated world. use extended key, which union keys, possibly other attributes, relations be matched,...
Childhood ataxia with central nervous system hypomyelination (CACH), or vanishing white matter leukoencephalopathy (VWM), is a fatal brain disorder caused by mutations in eukaryotic initiation factor 2B (eIF2B). eIF2B essential for protein synthesis and regulates translation response to cellular stresses. We performed mutagenesis introduce changes equivalent 12 human CACH/VWM three subunits of the from yeast (Saccharomyces cerevisiae) analyzed effects on cell growth, translation, gene...