A5035497987- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Erythropoietin and Anemia Treatment
- Trace Elements in Health
- Mass Spectrometry Techniques and Applications
- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- Systemic Lupus Erythematosus Research
- Rheumatoid Arthritis Research and Therapies
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Click Chemistry and Applications
- Radiopharmaceutical Chemistry and Applications
- Receptor Mechanisms and Signaling
- T-cell and B-cell Immunology
- Nitric Oxide and Endothelin Effects
- Advanced biosensing and bioanalysis techniques
- vaccines and immunoinformatics approaches
- Biochemical and Structural Characterization
- Analytical Chemistry and Chromatography
- Neurological diseases and metabolism
- Oxidative Organic Chemistry Reactions
- Medical Imaging Techniques and Applications
King's College London
2012-2025
University of Rajasthan
2025
Kempegowda Institute of Medical Sciences
2023
Institute of Medical Sciences
2023
Ganesh Shankar Vidyarthi Memorial Medical College
2023
Atal Bihari Vajpayee Institute of Medical Sciences and Dr. Ram Manohar Lohia Hospital
2023
Azienda Ospedaliera di Padova
2021
University of Padua
2021
Walter de Gruyter (Germany)
2021
Alberta Health Services
2017
The recently discovered iron regulatory peptide hormone hepcidin holds promise as a novel biomarker in metabolism disorders. To date, various mass spectrometry and immunochemical methods have been developed for its quantification plasma urine. Differences methodology analytical performance hinder the comparability of data. As first step towards method harmonization, several assays were compared. Worldwide eight laboratories participated urinary round robin which was analyzed. For both urine...
Brain glioblastoma and neurodegenerative diseases are still largely untreated due to the inability of most drugs cross blood–brain barrier (BBB). Nanoparticles have emerged as promising tools for drug delivery applications brain; in particular carbon nanotubes (CNTs) that shown an intrinsic ability BBB vitro vivo. Angiopep-2 (ANG), a ligand low-density lipoprotein receptor-related protein-1 (LRP1), has also results targeting brain using nanoparticles (NPs). Here, we investigate ANG-targeted...
Clinical applications of curcumin for the treatment cancer and other chronic diseases have been mainly hindered by its short biological half-life poor water solubility. Nanotechnology-based drug delivery systems potential to enhance efficacy poorly soluble drugs systemic delivery. This study proposes use poly(lactic-co-glycolic acid) (PLGA)-based polymeric oil-cored nanocapsules (NCs) loading colon in mice after injection. Formulations different oil compositions are prepared characterized...
The clinical application of EDV, a potent antioxidant drug approved for amyotrophic lateral sclerosis (ALS), is limited by its short biological half-life and poor water solubility necessitating hospitalization during intravenous infusion. Nanotechnology-based delivery constitutes powerful tool through inferring stability targeted improving bioavailability at the diseased site. Nose-to-brain offers direct access to brain bypassing blood barrier reducing systemic biodistribution. In this...
Abstract Muscarinic acetylcholine receptors were purified from rat forebrain and labeled with [3H]N-(2-chloroethyl)N-(2',3'-[3H2]propyl)-2-aminoethylbenzilate. Cleavage of the muscarinic a lysine-specific protease yielded labeled, glycosylated peptides about 130 200 residues in length, which came different receptor sequences. The probable cleavage sites are second intracellular loop extracellular or third loop. N-terminal disulfide-bonded to another part structure, supporting presence link...
SUMMARY The purpose of the study was to map dominant T cell epitope CB11 sequence CII in RT1u haplotype rats and determine if, when used as a synthetic peptide, it would induce tolerance protect against CIA. A corresponding residues 184–198 included fragment bovine identified proliferation assay using peptides an scanning system 15 amino acids, overlapping by 12 acids. This is bovine-specific, but cross-reacts with rat peptide. Minor epitopes were also autoantigenic. Use independently...
Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree equivalence achievable harmonization identification a commutable secondary material to accomplish this goal.We applied technical achieve developed Consortium for Harmonization Clinical Laboratory Results. Eleven measurement (5 mass spectrometry based 6 immunochemical based) quantified...
Molecular mimicry has been shown between two sequences of Klebsiella pneumoniae pulD secretion protein (DRDE) with HLA‐B27 (DRED) and pulA (pullulanase) enzyme (Gly‐X‐Pro) types I, III IV collagen respectively. IgG antibody levels in AS patients were elevated against 16mer synthetic peptides by linked immunosorbent assay (ELISA) compared to controls ( P < 0.001). ELISA assays K. grown the absence presence pullulan demonstrated significant IgA Increased I observed These observations could...
Summary In transfusional iron overload, extra‐hepatic distribution differs, depending on the underlying condition. Relative mechanisms of plasma non‐transferrin bound ( NTBI ) generation may account for these differences. Markers metabolism (plasma , labile iron, hepcidin, transferrin, monocyte SLC 40A1 [ferroportin]), erythropoiesis (growth differentiation factor 15, soluble transferrin receptor) and tissue hypoxia (erythropoietin) were compared in patients with Thalassaemia Major TM ),...
Carbon nanotubes (CNTs) have shown marked capabilities in enhancing antigen delivery to presenting cells. However, proper understanding of how altering the physical properties CNTs may influence uptake by cells, such as dendritic cells (DCs), has not been established yet. We hypothesized that multi-walled (MWNTs)-antigen conjugates, e.g. length and surface charge, can affect internalization MWNT-antigen DCs, hence induced immune response potency. For this purpose, pristine MWNTs (p-MWNTs)...
Azide- and alkyne-double functionalised graphene oxide (Click<sup>2</sup> GO) was synthesised characterised with ATR-FTIR, TGA, Raman spectroscopy.
Abstract Background Hepcidin concentrations measured by various methods differ considerably, complicating interpretation. Here, a previously identified plasma-based candidate secondary reference material (csRM) was modified into serum-based two-leveled sRM. We validated its functionality to increase the equivalence between for international standardization. Methods applied technical procedures developed International Consortium Harmonization of Clinical Laboratory Results. The sRM,...
Amyotrophic lateral sclerosis (ALS) presents a substantial challenge due to its complex nature, limited effective treatment options, and modest benefits from current therapies in slowing disease progression. This study explores the potential of intranasal (IN) delivery enhance CNS riluzole (RLZ), standard ALS which is subject blood-brain barrier efflux mechanisms. Additionally, impact elacridar (ELC), an pump inhibitor, on IN RLZ bioavailability was examined. To quantify vivo mice, [14C]-RLZ...
Human neuropsychiatric disorders are modelled in zebrafish (Danio rerio) to facilitate advancements drug discovery and for simulating complex neurological psychiatric disorders. The exhibits a variety of behaviors analogous human symptoms, such as social interaction, aggression, sleep patterns, learning, memory, anxiety. studies on their neuroendocrine system that mirrors the Hypothalamic-Pituitary-Adrenal (HPA) axis, can provide insights into illnesses. Here, we discuss selected examples...
Although immobilization of antigen-specific immunoglobulins onto matrix-assisted laser desorption/ionization (MALDI) targets allows the specific detection and enrichment an antigen from complex biological fluids, process antibody is not optimal. The principal reason that can bind to template in various orientations, many which block recognition. An affinity capture MALDI mass spectrometry methodology was developed by covalently immobilizing Fc receptor (recombinant protein G) gold for...
Intravenous iron-carbohydrate complex preparations (IVIPs) are non-interchangeable pro-drugs: their pharmacokinetics (PK) varies determined by semi-crystalline iron core and carbohydrate shell structures, influences pharmacodynamics (PD) thus efficacy safety. Examining PK/PD relationships of 3 IVIPs we identify a two-pathway model transient NTBI generation following single dose administration. 28 hypoferremic non-anemic patients randomized to 200mg as ferric carboxymaltose...
Abstract Hepcidin is a peptide hormone that functions as key regulator of mammalian iron metabolism. Biological levels are increased in end‐stage renal disease and during inflammation but suppressed hemochromatosis. Thus hepcidin have diagnostic importance. This study describes the development an analytical method for quantitative determination concentration clinical samples. The fragmentation was investigated using triple quadrupole linear ion trap mass spectrometers. A standard quantity...
Many chemopreventive agents have encountered bioavailability issues in pre-clinical/clinical studies despite high oral doses. We report here a new concept utilizing polycaprolactone implants embedded with test compounds to obtain controlled systemic delivery, circumventing and reducing the total administered dose. Compounds were released from vitro dose dependently for long durations (months), which correlated vivo release. Polymeric of curcumin significantly inhibited tissue DNA adducts...
Hepcidin is a peptide hormone that regulates the homeostasis of iron metabolism. The N-terminal domain hepcidin conserved amongst range species and capable binding CuII NiII through amino terminal copper–nickel motif (ATCUN). It has been suggested copper to may have biological relevance. In this study we investigated with model peptides containing ATCUN motif, fluorescently labelled using MALDI-TOF mass spectrometry. As albumin, it was found tetrapeptide models possessed higher affinity for...
Frogs such as Rana temporaria and Litoria aurea secrete numerous closely related antimicrobial peptides (AMPs) an effective chemical dermal defence. Damage or penetration of the bacterial plasma membrane is considered essential for AMP activity properties are commonly ascribed to their ability form secondary amphipathic, α-helix conformations in mimicking milieu. Nevertheless, despite high similarity physical preference adopting conformations, spectrum potency AMPs often varies considerably....