A5016476706- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- ATP Synthase and ATPases Research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Photosynthetic Processes and Mechanisms
- Chemical Synthesis and Analysis
- Mitochondrial Function and Pathology
- Cancer Research and Treatments
- Advanced NMR Techniques and Applications
- Gut microbiota and health
- Cystic Fibrosis Research Advances
- Biochemical and Structural Characterization
- Electron Spin Resonance Studies
- Pancreatic function and diabetes
- Chemokine receptors and signaling
- Parkinson's Disease Mechanisms and Treatments
- Photoreceptor and optogenetics research
- Tracheal and airway disorders
- Microencapsulation and Drying Processes
- Supramolecular Self-Assembly in Materials
- Advanced Fluorescence Microscopy Techniques
- Biopolymer Synthesis and Applications
King's College London
2010-2018
Université de Strasbourg
2016-2018
Institut de Chimie
2018
Institut de Chimie de Strasbourg
2015-2017
Centre National de la Recherche Scientifique
2016-2017
Université de Toulouse
2007-2009
Université Toulouse III - Paul Sabatier
2009
Wageningen University & Research
2006
<h3>Background</h3> Culture-independent analysis of the respiratory secretions people with cystic fibrosis (CF) has identified many bacterial species not previously detected using culture in this context. However, little is known about their clinical significance or persistence CF airways. <h3>Methods</h3> The authors characterised viable communities sputum collected from 14 patients at monthly intervals over 1 year a molecular community profiling technique—terminal restriction fragment...
The interaction of antimicrobial peptides (AMPs) with the inner membrane Gram-negative bacteria is a key determinant their abilities to exert diverse bactericidal effects. Here we present molecular level understanding initial target for two cationic α-helical AMPs that share structural similarities but have ten-fold difference in antibacterial potency towards bacteria. binding and insertion from solution pleurocidin or magainin 2 membranes representing bacteria, comprising mixture 128...
The antimicrobial peptide GL13K encompasses 13 amino acid residues and has been designed optimized from the salivary protein BPIFA2 to exhibit potent bacteriocidal anti-biofilm activity against Gram-negative Gram-positive bacteria as well anti-lipopolysaccharide in vitro vivo. Here, was analyzed a variety of membrane environments by circular dichroism spectroscopy high-resolution multidimensional solution nuclear magnetic resonance (NMR) spectroscopy. Whereas absence membranes random coil...
Obtaining an in-depth understanding of the arms races between peptides comprising innate immune response and bacterial pathogens is fundamental interest will inform development new antibacterial therapeutics. We investigated whether a whole organism view antimicrobial peptide (AMP) challenge on Escherichia coli would provide suitably sophisticated perspective AMP mechanism action. Selecting structurally physically related AMPs but with expected differences in bactericidal strategy, we...
Nonviral vectors that harness the change in pH endosomes, are increasingly being used to deliver cargoes, including nucleic acids, into mammalian cells. Here we present evidence pK(a) of beta-NH(2) 2,3-diaminopropionic acid (Dap) is sufficiently lowered, when Dap incorporated peptides, its protonation state sensitive changes occur during endosomal acidification. The lowered around 6.3 stabilized by increased electron-withdrawing effect peptide bonds, intermolecular hydrogen bonding and from...
The alpha chemokine receptor CXCR4 is up-regulated in certain types of breast cancer. Truncation the C-terminus this alters cell morphology and increases invasiveness metastatic potential. Here, to better understand effects expression truncation cancer cells, we have used high resolution magic angle spinning (HR-MAS) NMR studies rat carcinoma MtLn3E cells characterize metabolite complement heterologously expressing human or its C-terminal mutant, Δ34-CXCR4. Notable reductions choline levels...
Abstract Circular Dichroism data are often decomposed into their constituent spectra to quantify the secondary structure of peptides or proteins but estimation content fails when light scattering leads spectral distortion. If peptide-induced liposome self-association occurs, subtracting control curves cannot correct for this. We show that if cause is independent from peptide structural changes, CD can be corrected using principal component analysis (PCA). The itself analysed and found in...
Cationic amphipathic pH responsive peptides possess high in vitro and vivo nucleic acid delivery capabilities function by forming a non-covalent complex with cargo, protecting it from nucleases, facilitating uptake via endocytosis responding to endosomal acidification being released the inserting into disordering membranes. We have designed synthesised show how Coulombic interactions between ionizable 2,3-diaminopropionic (Dap) side chains can be manipulated tune functional response of...
ESR (or EPR) spectroscopy on spin-labeled site-directed cysteine mutants is ideally suited for structural studies of membrane proteins due to its high sensitivity and low demands with respect sample purity preparation. Many features can be inferred from the spectral line shape an spectrum, but analysis spectra complicated when multiple sites different shapes are present. Here, we present a method decompose spectrum doubly labeled peptide that composed singly labeled, noninteracting component...
Gene transfer into hCD34+ hematopoietic stem/progenitor cells (HSPCs) using HIV-1-based lentiviral vectors (LVs) has several therapeutic applications ranging from monogenic diseases, infectious diseases and cancer. In such context, the gene therapy could be improved by enhancing transduction levels of target reducing amount LVs used on for greater safety reduced costs. We recently identified a new cationic amphipathic peptide, Vectofusin-1, with viral capacity, enabling higher low amounts...